Anti apo2.7 pe

The following antibodies were used for flow cytometry analysis: anti-Apo2.7-PE, anti-CD5-FITC, anti-CD19-APC and control IgG1-FITC mAbs were purchased from BD Biosciences (Le Pont de Claix, France). Analysis of protein expression was conducted by immunoblotting using the following primary antibodies: anti-Bcl-2, anti-IκBα and anti-phosho-IκBα (Cell Signaling, Saint Quentin en Yvelines, France), Anti-Mcl-1 (S19) (Santa Cruz Biotechnology, Santa Cruz, CA), anti-Bcl-x_L (BD Biosciences, Le Pont de Claix, France), Anti-NF-κB p52 Antibody and anti-actin (Merck Millipore, Lyon, France). ABT-199 was kindly provided by Abbvie Laboratories (North Chicago, IL, USA) and the selective BTK inhibitor ibrutinib (PCI-32765) was obtained from Selleck Chemicals (Souffelweyersheim, France).

RITA and nutlin3a were purchased from Santa Cruz Technology (Santa Cruz, CA, USA) and Sigma (Saint-Quentin Fallavier, France), respectively. Anti-Apo2.7-PE, anti-CD138-PE, control IgG1-PE and anti-BrdU-FITC mAbs were purchased from BD Biosciences (Le Pont de Claix, France). Anti-DR5-PE was purchased from eBioscience. Annexin V was purchased from Beckman Coulter (Immunotech, Marseilles, France).