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Mirax desk

Manufactured by Zeiss
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Sourced in Germany

The Mirax Desk is a compact and versatile lab equipment designed for a range of microscopy applications. It provides a stable and adjustable platform to support various microscopes and accessories. The Mirax Desk features a sturdy construction and adjustable height to accommodate different user needs and provide a comfortable working environment.

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Lab products found in correlation

Mirax viewer, by Zeiss (3 mentions) Axiovision 4, by Zeiss (3 mentions) Histocore spectra st, by Leica (1 mentions) K3468, by Agilent Technologies (1 mentions) Protein block, by Agilent Technologies (1 mentions) E0431, by Agilent Technologies (1 mentions) Ab5694, by Abcam (1 mentions) Pannoramic viewer version 1, by 3DHISTECH (1 mentions) Ab10983, by Abcam (1 mentions) Discovery xt, by Roche (1 mentions) Hematoxylin, by Merck Group (1 mentions) Potassium ferrocyanide, by Merck Group (1 mentions) Dibutylphthalate polystyrene xylene (dpx), by Merck Group (1 mentions) Sprayer, by HTX Technologies (1 mentions) Fleximaging, by Bruker (1 mentions) Rapiflex maldi tissuetyper, by Bruker (1 mentions) Fleximaging 4, by Bruker (1 mentions) Solarix 7 t ft icr mass spectrometer, by Bruker (1 mentions) Eclipse 80i, by Zeiss (1 mentions) Nanotom s, by Phoenix Pharmaceuticals (1 mentions)

Close spelling variants of this product

Mirax (17 citations) Mirax Desk scanner (10 citations) MIRAX Desk Digital Slide Scanner (4 citations) Mirax Digital Desk Scanner (2 citations) Mirax Desk slide scanner (2 citations)

14 protocols using mirax desk

1

Cresyl Violet Staining for SIMS Imaging

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After SIMS imaging the tissue was stained with Cresyl violet (Nissl stain) and scanned using a Mirax DESK digital slide scanner (Zeiss, Germany).
Škrášková K., Khmelinskii A., Abdelmoula W.M., De Munter S., Baes M., McDonnell L., Dijkstra J, & Heeren R.M. (2015). Precise Anatomic Localization of Accumulated Lipids in Mfp2 Deficient Murine Brains Through Automated Registration of SIMS Images to the Allen Brain Atlas. Journal of the American Society for Mass Spectrometry, 26(6), 948-957.
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2

MALDI-MSI-Assisted Histological Analysis

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Following MALDI-MSI, the matrix was washed off the section surface in 70% ethanol for 4 min. The sections were subsequently stained with hematoxylin–eosin in a HistoCore SPECTRA ST multistainer (Leica, Germany), using the ST Infinity H&E staining system (ref. 3801098, Leica, Germany), coverslipped, examined by light microscopy, and scanned using a digital slide scanner equipped with a 20 × magnification objective (MIRAX DESK, Zeiss, Germany).
Blutke A., Sun N., Xu Z., Buck A., Harrison L., Schriever S.C., Pfluger P.T., Wiles D., Kunzke T., Huber K., Schlegel J., Aichler M., Feuchtinger A., Matiasek K., Hauck S.M, & Walch A. (2020). Light sheet fluorescence microscopy guided MALDI-imaging mass spectrometry of cleared tissue samples. Scientific Reports, 10, 14461.
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3

Immunohistochemical Analysis of Hepatic α-SMA

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Five-µm-thick hepatic tissue sections were deparaffinized in xylene and rehydrated serially with alcohol and water, followed by microwave antigen retrieval for 20 min at 98 °C in 10 mM sodium citrate buffer (0.05% tween 20, pH 6.0). Endogenous peroxidase was blocked with fresh 0.3% hydrogen peroxide in phosphate buffered saline (PBS) for ten minutes at room temperature. Sections were blocked 2 h with protein block (DAKO, Belgium) in PBS containing 0.5% triton and were incubated overnight at 4 °C in the presence of a specific alpha-smooth muscle actin antibody (α-SMA, 1/200, ab5694, Abcam, UK). After being washed three times in PBS, sections were incubated with biotinylated secondary swine anti-rabbit antibody (E0431, DAKO, Belgium) for 1 h and with streptavidine-HRP (1/800, P0397, DAKO, Belgium) for 30 min. α-SMA was visualized using DAB (K3468, DAKO, Belgium) and sections were counterstained with hematoxylin. Sections were observed at 20X magnification Mirax Desk and Mirax viewer, Carl Zeiss MicroImaging, Germany).
Cops J., Mullens W., Verbrugge F.H., Swennen Q., De Moor B., Reynders C., Penders J., Achten R., Driessen A., Dendooven A., Rigo J.M, & Hansen D. (2018). Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function. Scientific Reports, 8, 17757.
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4

Quantifying Organ Fibrosis via Trichrome Staining

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Five µm thickness sections of liver, kidney and heart tissue, subjected to the Masson trichrome staining method, were scanned using the Mirax Desk (Carl Zeiss MicroImaging, Germany). Fibrosis was assessed at 20X magnification in four randomly chosen sections in each organ per rat, by outlining the area of collagen deposition and excluding blood vessels, as described previously15 (link),43 (link). Quantification of percentage collagen was performed by calculating the ratio of the area of collagen deposition to the global area using an automated image analysis program (AxioVision 4.6, Carl Zeiss MicroImaging, Germany)15 (link).
Cops J., Mullens W., Verbrugge F.H., Swennen Q., De Moor B., Reynders C., Penders J., Achten R., Driessen A., Dendooven A., Rigo J.M, & Hansen D. (2018). Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function. Scientific Reports, 8, 17757.
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5

Lung Tissue Processing and Histology

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The right lung was snap frozen in liquid nitrogen for further analysis. The left lung was fixed under a constant pressure of 20 cm by intratracheal instillation of 6% paraformaldehyde and using systematic uniform random sampling embedded into paraffin for Hematoxylin and Eosin (H&E)-stained histological analysis and immunohistochemistry. Images of stained sections were obtained using a Mirax Desk (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) slide scanner and analysed using Pannoramic Viewer version 1.15.2 (3DHistech Kft, Budapest, Hungary).
Hellbach K., Meinel F.G., Conlon T.M., Willer K., Yaroshenko A., Velroyen A., Braunagel M., Auweter S., Reiser M.F., Eickelberg O., Pfeiffer F, & Yildirim A.Ö. (2018). X-Ray Dark-field Imaging to Depict Acute Lung Inflammation in Mice. Scientific Reports, 8, 2096.
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6

Quantifying UCP1 and Adipocyte Size in Gonadal Fat

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Gonadal fat samples were dissected and subsequently fixed and stored in 4% paraformaldehyde. After dehydration, tissues were embedded in paraffin and cut into 5 μm slices. Immunohistochemical staining was performed on a Discovery XT automated stainer (Ventana, Medical Systems) using rabbit anti-UCP1 antibody (1:1500; ab10983, Abcam). Signal detection was performed using the diaminobenzidine as a chromogen (Ventana Medical Systems). Images obtained by UCP1 and H&E staining were analyzed using the commercially available image analysis software Definiens Developer XD2.1 (Definiens AG; München, Germany). For this purpose, all stained slides were scanned at 20× objective magnification using a Mirax Desk digital slide scanner (Carl Zeiss MicroImaging, Munich, Germany), and the resulting images were imported into image analysis software. A specific rule set was defined to detect the stained tissue area (UCP1) or the adipocytes (H&E) within each defined region. The quantified parameter was the percentage of the UCP1 stained area related to the defined tissue area. For H&E staining the calculated parameter was the mean size of the adipocytes.
Norheim F., Hasin-Brumshtein Y., Vergnes L., Krishnan K.C., Pan C., Seldin M.M., Hui S.T., Mehrabian M., Zhou Z., Gupta S., Parks B.W., Walch A., Reue K., Hofmann S.M., Arnold A.P, & Lusis A.J. (2019). Gene-by-sex interactions in mitochondrial functions and cardio-metabolic traits. Cell metabolism, 29(4), 932-949.e4.
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7

H&E Staining of ITO Slides for MSI

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After MSI measurements, all ITO slides were washed with 70% methanol for 30 s to remove
matrix before hematoxylin and eosin (H&E) staining. The samples were dried in a vacuum
desiccator for 10 min, followed by rinsing in hematoxylin (Merck, Darmstadt, Germany) for
1 min, 10 min in tap water, 1 min in distilled water, and another 3 min in eosin (Merck,
Darmstadt, Germany). Then, all sections were dehydrated in a graded ethanol series (70%,
2 × 96%, 2 × 100%, 2 min each) and finally rinsed for another 2 min in
xylene. Coverslips were mounted onto the slides using a Tissue-Tek SCA 4764 Coverslipper
(Sakura Finetek, The Netherlands). After air-drying overnight at room temperature, the
H&E stained slides were scanned using a digital slide scanner (Mirax Desk, Zeiss,
Jena, Germany) and coregistered in FlexImaging to the MSI data using the previously
applied fiducial markers. Then, an expert in vascular pathology annotated plaque regions
digitally in the scanned images.
Cao J., Goossens P., Martin-Lorenzo M., Dewez F., Claes B.S., Biessen E.A., Heeren R.M, & Balluff B. (2020). Atheroma-Specific Lipids in ldlr–/– and apoe–/– Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging. Journal of the American Society for Mass Spectrometry, 31(9), 1825-1832.
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8

Quantifying Kidney Fibrosis via Trichrome Staining

Cited 1 time
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Kidney sections of five μm thick were stained using the Masson trichrome staining method. Sections were scanned using the Mirax Desk and observed at 20-times magnification using the Mirax viewer (Carl Zeiss MicroImaging, Germany). Fibrosis was assessed in four randomly chosen sections per rat, as previously described [25 (link), 26 (link)]. The area of collagen deposition was outlined and quantified using an automated image analysis program (AxioVision 4.6, Carl Zeiss MicroImaging, Germany). Percentage fibrosis was calculated as the ratio of the area of collagen deposition to the global area.
Cops J., Mullens W., Verbrugge F.H., Swennen Q., Reynders C., Penders J., Rigo J.M, & Hansen D. (2018). Selective abdominal venous congestion to investigate cardiorenal interactions in a rat model. PLoS ONE, 13(5), e0197687.
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9

Identification of USPIO-Containing Islets

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After fixation of isolated kidneys and performing ex vivo MRI, samples were embedded in paraffin and 5 µm thick sections were made. Paraffin sections were deparaffinised and dehydrated by passing through a graded alcohol series. To identify USPIO-containing islets, Masson Trichrome and Perl’s staining were performed. Images were acquired using a Mirax Desk (Carl Zeiss, Göttingen, Germany).
Garcia Ribeiro R.S., Gysemans C., da Cunha J.P., Manshian B.B., Jirak D., Kriz J., Gallo J., Bañobre-López M., Struys T., De Cuyper M., Mathieu C., Soenen S.J., Gsell W, & Himmelreich U. (2018). Magnetoliposomes as Contrast Agents for Longitudinal in vivo Assessment of Transplanted Pancreatic Islets in a Diabetic Rat Model. Scientific Reports, 8, 11487.
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10

Prussian Blue Staining of SPIO-Labeled Islets

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Mouse kidneys were isolated on days two and five post-PI transplantation, fixed overnight in 10% neutral buffered formalin, and embedded in paraffin. Subsequently, 5 μm-thick sections were made. Paraffin sections were de-paraffinized and dehydrated by passing them through a graded alcohol series. To identify SPIO-containing islets, Prussian blue staining was performed. The slides were placed in a mixture containing an equal volume of 10% potassium ferrocyanide (Sigma) and 20% HCl (Vel Labs, Leuven, Belgium) for 20 min at room temperature, and then rinsed in tap water for 15 min. After 10 min of nuclear fast red counterstaining, the slides were passed through a graded series of alcohol and xylene before mounting with DPX (Sigma). Images were acquired using a Mirax Desk (Carl Zeiss, Göttingen, Germany).
Ribeiro R.S., Ketkar-Atre A., Yin T., Louchami K., Struys T., Lambrichts I., Sener A., Malaisse W.J., De Cuyper M, & Himmelreich U. (2018). Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes. Journal of Personalized Medicine, 8(1), 12.
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