Log In Sign up
The largest database of trusted experimental protocols

Scm peg5k mal

Manufactured by Creative PEGWorks
Visit Supplier

SCM-PEG5k-Mal is a polyethylene glycol (PEG) derivative with a maleimide functional group. It is used for the covalent attachment of PEG to other molecules, such as proteins or peptides, through thiol-maleimide chemistry.

Automatically generated - may contain errors

Lab products found in correlation

Pd 10 column, by GE Healthcare (8 mentions) Chelex 100 resin, by Merck Group (7 mentions) Nhs fluorescein, by Merck Group (5 mentions) P scn bn nota, by Macrocyclics (5 mentions) Absolute ethanol, by Thermo Fisher Scientific (4 mentions) Chelex 100 resin 50 100 mesh, by Merck Group (3 mentions) Kaiser test kit, by Merck Group (3 mentions) Traut s reagent 2 iminothiolane hcl, by Thermo Fisher Scientific (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Dodium citrate dehydrate, by Merck Group (2 mentions) Hematoxylin staining solution, by Merck Group (2 mentions) Alexafluor488 and cy3 labeled secondary antibodies, by Jackson ImmunoResearch (2 mentions) Irdye800cw nhs, by LI COR (2 mentions) Nota scn, by Macrocyclics (2 mentions) Cy3 labeled secondary antibody, by Jackson ImmunoResearch (2 mentions) Sunitinib malate, by Bio-Techne (2 mentions) Cyclohexane, by Thermo Fisher Scientific (1 mentions) Pbs 1x, by Thermo Fisher Scientific (1 mentions) Sunitinib, by LC Laboratories (1 mentions) Absolute ethanol, by Merck Group (1 mentions)

8 protocols using scm peg5k mal

1

Synthesis and Characterization of Functionalized Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). p-SCN-Bn-NOTA (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, hematoxylin staining solution, and Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-Aminopropyl)triethoxysilane (APS), Cetyltrimethylammonium chloride solution (CTAC, 25 wt %), copper(II) chloride (CuCl2), sodium sulfide nonahydrate (Na2S·9H2O) and dodium citrate dehydrate were purchased from Sigma-Aldrich (St. Louis, MO). AlexaFluor488- and Cy3-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, sodium chloride (NaCl), doxorubicin hydrochloride (DOX) were purchased from Fisher Scientific. SCM-PEG5k-Mal was obtained from Creative PEGworks. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
Chen F., Hong H., Goel S., Graves S.A., Orbay H., Ehlerding E.B., Shi S., Theuer C.P., Nickles R.J, & Cai W. (2015). In Vivo Tumor Vasculature Targeting of CuS@MSN Based Theranostic Nanomedicine. ACS Nano, 9(4), 3926-3934.
+ Open protocol
+ Expand
2

Synthesis and Characterization of cRGDyK Peptide Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyclo-(Arg-Gly-Asp-D-Tyr-Lys) peptide (cRGDyK) was procured from Peptide International (Louisville, KY). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, cyclohexane and sodium chloride (NaCl) were purchased from Fisher Scientific (Pittsburg, PA). Sunitinib was purchased from LC Laboratories (Woburn, MA). SCM-PEG5k-Mal was obtained from Creative PEG works (Winston Salem, NC). p-SCN-Bn-NOTA was acquired from Macrocyclics, Inc.(Dallas, TX). NHS-Fluorescein, Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate(TEOS), ammonia (NH3.H2O), Igepal CO-520 (NP-5), triethylamine (TEA), (3-Aminopropyl)triethoxysilane (APS), dimethyl sulfoxide (DMSO), cetyltrimethylammonium chloride (CTAC, 25 wt%) and Kaiser test kit were purchased from Sigma-Aldrich (St. Louis, MO). Traut’s Reagent (2-Iminothiolane. HCl) and PBS (1X) were purchased from Thermo Scientific (Rockford, IL). Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
Chakravarty R., Goel S., Hong H., Chen F., Valdovinos H.F., Hernandez R., Barnhart T.E, & Cai W. (2015). Functionalized Hollow Mesoporous Silica Nanoparticles for Tumor Vasculature Targeting and PET Image-Guided Drug Delivery. Nanomedicine (London, England), 10(8), 1233-1246.
+ Open protocol
+ Expand
3

Synthesis and Functionalization of Lanthanide-Doped Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-aminopropyl)triethoxysilane (APTES), cetyltrime-thylammonium chloride solution (CTAC, 25 wt.%), absolute ethanol, sodium chloride (NaCl), sodium hydroxide (99.99%), oleic acid, 1-octadecene (technical grade, 90%), ammonia, yttrium(III) chloride hexahydrate, ytterbium(III) chloride hexahydrate, thulium(III) chloride, Igepal CO-520, and ammonium fluoride were obtained from Sigma-Aldrich and used without further purification. Iron chloride hexahydrate (FeCl3·6H2O, > 99%) was purchased from Acros. PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). SCM-PEG5k-Mal was obtained from Creative PEGworks. QD705 (1 µM in decane) was purchased from Life Technology. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals.
Chen F., Goel S., Shi S., Barnhart T.E., Lan X, & Cai W. (2018). General synthesis of silica-based yolk/shell hybrid nanomaterials and in vivo tumor vasculature targeting. Nano research, 11(9), 4890-4904.
+ Open protocol
+ Expand
4

Bioconjugation of TRC105 with IR Dye 800CW

Check if the same lab product or an alternative is used in the 5 most similar protocols
TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). IR Dye 800CW-NHS (NHS denotes N-hydroxysuccinimide) ester was acquired from LI-COR Biosciences Co. (Lincoln, NE). SCM-PEG5k-Mal was obtained from Creative PEGworks. NOTA-SCN (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), ammonia (NH3·H2O), triethylamine (TEA), 3-aminopropyl)triethoxysilane (APS), dimethyl sulfoxide, cetyltrimethylammonium chloride (CTAC, 25 wt %), and Kaiser test kit were purchased from Sigma-Aldrich (St. Louis, MO). Traut’s reagent (2-Iminothiolane·HCl) and Ellman’s reagent (5,5′-dithiobis(2-nitrobenzoic acid) or DTNB) were purchased from Fisher Scientific. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
Chen F., Nayak T.R., Goel S., Valdovinos H.F., Hong H., Theuer C.P., Barnhart T.E, & Cai W. (2014). In Vivo Tumor Vasculature Targeted PET/NIRF Imaging with TRC105(Fab)-Conjugated, Dual-Labeled Mesoporous Silica Nanoparticles. Molecular Pharmaceutics, 11(11), 4007-4014.
+ Open protocol
+ Expand
5

Bioconjugation of TRC105 with IR Dye

Check if the same lab product or an alternative is used in the 5 most similar protocols
TRC105 was provided by TRACON Pharmaceuticals
Inc. (San Diego, CA). PD-10 columns were purchased from GE Healthcare
(Piscataway, NJ). IR Dye 800CW-NHS (NHS denotes N-hydroxysuccinimide) ester was acquired from LI-COR Biosciences Co.
(Lincoln, NE). SCM-PEG5k-Mal was obtained from Creative
PEGworks. NOTA-SCN (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic
acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein,
Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS),
ammonia (NH3·H2O), triethylamine (TEA),
3-aminopropyl)triethoxysilane (APS), dimethyl sulfoxide, cetyltrimethylammonium
chloride (CTAC, 25 wt %), and Kaiser test kit were purchased from
Sigma-Aldrich (St. Louis, MO). Traut’s reagent (2-Iminothiolane·HCl)
and Ellman’s reagent (5,5′-dithiobis(2-nitrobenzoic
acid) or DTNB) were purchased from Fisher Scientific. Water and all
buffers were of Millipore grade and pretreated with Chelex 100 resin
to ensure that the aqueous solution was free of heavy metals. All
chemicals were used as received without further purification.
Chen F., Nayak T.R., Goel S., Valdovinos H.F., Hong H., Theuer C.P., Barnhart T.E, & Cai W. (2014). In Vivo Tumor Vasculature Targeted PET/NIRF Imaging with TRC105(Fab)-Conjugated, Dual-Labeled Mesoporous Silica Nanoparticles. Molecular Pharmaceutics, 11(11), 4007-4014.
+ Open protocol
+ Expand
6

Bioconjugation of TRC105 with NOTA

Check if the same lab product or an alternative is used in the 5 most similar protocols
TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). p-SCN-Bn-NOTA (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7- triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, hematoxylin staining solution, and Chelex 100 resin (50~100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-Aminopropyl)triethoxysilane (APS), Cetyltrimethylammonium chloride solution (CTAC, 25 wt%), copper(II) chloride (CuCl2), sodium sulfide nonahydrate (Na2S·9H2O) and dodium citrate dehydrate were purchased from Sigma-Aldrich (St. Louis, MO). AlexaFluor488- and Cy3-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, sodium chloride (NaCl), doxorubicin hydrochloride (DOX) were purchased from Fisher Scientific. SCM-PEG5k-Mal was obtained from Creative PEGworks. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
Chen F., Hong H., Goel S., Graves S.A., Orbay H., Ehlerding E.B., Shi S., Theuer C.P., Nickles R.J, & Cai W. (2015). In Vivo Tumor Vasculature Targeting of CuS@MSN Based Theranostic Nanomedicine. ACS Nano, 9(4), 3926-3934.
+ Open protocol
+ Expand
7

Radiolabeled Nanomedicine Synthesis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
K3-VEGF121 was purchased from Corp. (Piscataway, NJ). p-SCN-Bn-NOTA was acquired from Macrocyclics, Inc. (Dallas, TX). Mal-PEG5k-SCM was purchased from Creative PEGworks (Winston Salem, NC). NHS-fluorescein and Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), triethylamine (TEA), 3-aminopropylsilanetriol (APS), hexadecyl trimethyl ammonium chloride (CTAC, 25 wt%) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO). Cy3-labeled secondary antibody was purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). Sunitinib malate was purchased from Tocris Biosciences (Minneapolis, MN). PD-10 columns were bought from GE Healthcare (Piscataway, NJ). Absolute ethanol and sodium chloride (NaCl) were obtained from Fisher Scientific. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
Goel S., Chen F., Hong H., Valdovinos H.F., Hernandez R., Shi S., Barnhart T.E, & Cai W. (2014). VEGF121-Conjugated Mesoporous Silica Nanoparticle: A Tumor Targeted Drug Delivery System. ACS Applied Materials & Interfaces, 6(23), 21677-21685.
+ Open protocol
+ Expand
8

Preparation and Characterization of K3-VEGF121 Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols
K3-VEGF121 was purchased from
Corp. (Piscataway, NJ). p-SCN-Bn-NOTA was acquired
from Macrocyclics, Inc. (Dallas, TX). Mal-PEG5k-SCM was
purchased from Creative PEGworks (Winston Salem, NC). NHS-fluorescein
and Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate
(TEOS), triethylamine (TEA), 3-aminopropylsilanetriol (APS), hexadecyl
trimethylammonium chloride (CTAC, 25 wt %) and dimethyl sulfoxide
(DMSO) were obtained from Sigma-Aldrich (St. Louis, MO). Cy3-labeled
secondary antibody was purchased from Jackson Immunoresearch Laboratories,
Inc. (West Grove, CA). Sunitinib malate was purchased from Tocris
Biosciences (Minneapolis, MN). PD-10 columns were bought from GE Healthcare
(Piscataway, NJ). Absolute ethanol and sodium chloride (NaCl) were
obtained from Fisher Scientific. Water and all buffers were of Millipore
grade and pretreated with Chelex 100 resin to ensure that the aqueous
solution was free of heavy metals. All chemicals were used as received
without further purification.
Goel S., Chen F., Hong H., Valdovinos H.F., Hernandez R., Shi S., Barnhart T.E, & Cai W. (2014). VEGF121-Conjugated Mesoporous Silica Nanoparticle: A Tumor Targeted Drug Delivery System. ACS Applied Materials & Interfaces, 6(23), 21677-21685.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!