Saponin solution

Manufactured by Merck Group

Sourced in Germany, France, United States

Saponin solution is a laboratory reagent composed of saponin, a natural surfactant derived from plants. It is used in various scientific applications to facilitate the extraction and solubilization of biological samples, particularly cell membranes and lipid-containing compounds.

Automatically generated - may contain errors

Lab products found in correlation

Anti il 17 apc, by Thermo Fisher Scientific (1 mentions) Anti cd3, by Thermo Fisher Scientific (1 mentions) Brefeldin a, by Merck Group (1 mentions) Anti cd28, by BioLegend (1 mentions) Formaldehyde, by Ted Pella (1 mentions) Brilliant violet 605, by BioLegend (1 mentions) Ccr2 clone 475301, by R&D Systems (1 mentions) Apc cy7, by R&D Systems (1 mentions) Rtk4530, by BioLegend (1 mentions) Cd11c, by R&D Systems (1 mentions) Cd11b, by R&D Systems (1 mentions) Protein labeling kit, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Pe cy7, by BioLegend (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) Zombie aqua, by BioLegend (1 mentions) Nanoboosters, by Proteintech (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Netrin 1 rat anti mouse monoclonal antibody clone, by R&D Systems (1 mentions)

6 protocols using saponin solution

Cytokine Production in Stimulated Tregs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tregs after CD45RA stimulation were cultured with anti-CD3 (1 μg/ml)(eBioscience) and anti-CD28 (5 μg/ml) (BioLegend, San Diego, CA, USA) in presence of Brefeldin A (10 μg/ml) (Sigma) for 48 h at 37°C. Cells were collected, fixed and permeabilised with 0,5% saponin solution (Sigma). Intracellular staining was carried out using anti-IL-10-PE (Becton Dickinson), anti-IL-4-PeCy5.5 (Becton Dickinson), anti-IFN-γ-FITC (Becton Dickinson) and anti-IL-17-APC (eBioscience) antibodies. Cells were acquired by FACSCanto flow cytometer and analysed by FACSDiva software.

Zizzari I.G., Martufi P., Battisti F., Rahimi H., Caponnetto S., Bellati F., Nuti M., Rughetti A, & Napoletano C. (2015). The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions. PLoS ONE, 10(7), e0132617.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry for Flavivirus Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Staining with Zombie Aqua (BioLegend) or 7-AAD (eBiosciences) viability dyes excluded dead cells from general analysis. Specific staining of surface markers distinguished cell types using monoclonal antibodies from BioLegend, if not stated otherwise: CCR2 (clone 475301, R&D), CD11b (M1/70), CD11c (N418), CD45 (30-F11), CD80 (16-10A1), CD86 (PO3), CD103 (2E7), Ly6C (AL21), Ly6G (1A8), MHC II (I-A/I-E, M5/114.15.2), and Armenian hamster IgG (HTK888) or rat IgG2b isotype controls (RTK4530, BioLegend; or 141945, R&D), which were conjugated to PacificBlue, Brilliant Violet 605, PE, PE-CF594, PE-Cy7, Alexa Fluor 700, APC-Cy7, or biotin. Antibody stains were performed in D-PBS with no Ca⁺⁺/Mg⁺⁺ containing 2% FBS and 2 mM EDTA (LifeTech). Biotinylated antibodies were visualized using streptavidin conjugated to Brilliant Violet 605 or PE-Cy7 (BioLegend). After fixation with 2% formaldehyde (Ted Pella), cells were permeabilized with 1 mg/ml saponin solution (Sigma) containing 2% FBS and 1% normal mouse serum obtained from steady-state mice. Intracellular staining for DENV proteins E (4G2, ATCC) and NS3 (E1D8, [35] (link)), which were conjugated to Alexa Fluor 488 or Alexa Fluor 647, respectively, using protein-labeling kits (LifeTech), identified DENV2-infected cells. Intracellular staining for Langerin (4C7) was used to further dissect cell populations.

Schmid M.A, & Harris E. (2014). Monocyte Recruitment to the Dermis and Differentiation to Dendritic Cells Increases the Targets for Dengue Virus Replication. PLoS Pathogens, 10(12), e1004541.

+ Open protocol

+ Expand

Multicolor dSTORM Imaging of HIV Envelope

Check if the same lab product or an alternative is used in the 5 most similar protocols

COS-7 cells expressing CCR5-YFP or CXCR4-YFP and CD4-mRFP were incubated with non-labelled JRFL or HXB2 virions respectively for 10minutes at 37°C. After a single PBS wash to remove non-primed virions, cells were fixed with 3.7% PFA (Sigma) for 20 minutes at room temperature followed by 5 min permeabilisation with a 2% Saponin solution (Sigma), After 1h blocking with 4% BSA (Sigma), Nanoboosters (Chromotek, Germany) targeting the YFP (GFP_Booster_Atto488) or the RFP (RFP_Booster_Atto594) were added in 1:100 final concentration in blocking buffer and incubated for one additional hour. S Flag signal was introduced in HXB2, thus these virons were labelled with an mouse-anti-FLAG antibody and an anti-mouse Alexa 405 secondary. JRFL was detected with a 2G12 primary antibody and a anti-human- Alexa 633 secondary. These combination of colours was selected to avoid spectral overlap between the three channels (CD4, Co-Receptors and Env) and sequential acquisition was performed from the longest wavelength until the shortest to avoid collateral photobleaching during each dSTORM acquisition (see below).

Iliopoulou M., Nolan R., Alvarez L., Watanabe Y., Coomer C.A., Jakobsdottir G.M., Bowden T.A, & Padilla-Parra S. (2018). A dynamic three step mechanism drives the HIV-1 prefusion reaction. Nature structural & molecular biology, 25(9), 814-822.

+ Open protocol

+ Expand

Netrin-1 Expression in Lymphoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells in RPMI medium (26 nodal lymphomas and five normal lymph nodes) were formalin-fixed for 15 min, and permeabilized for 10 min with a 0.3% saponin solution (Sigma Aldrich, France). After washing, cells were incubated for 30 min with netrin-1 rat anti-mouse monoclonal antibody (clone, diluted 1:100, R&D Systems, Minneapolis, USA), and 30 min with a secondary polyclonal goat anti-rat/mouse FITC (clone F047902-2, Agilent, Carpinteria, CA). Fluorescence was measured after an ultimate washing, with a cytometer Accuri (Becton Dickinson, France). Results were analyzed with the BD Accuri C6 Software ^®® (Becton Dickinson, Le Pont de Claix, France). To discriminate fluorescence intensity between culture cells, evaluation of difference in mean fluorescence intensity (ΔMFI) was performed, as published in Fellman et al. [26 (link)], according to this formula: ΔMFI = (MFI Netrin-1–MFI control) / MFI control. Control cells were incubated with the secondary antibody only.

Tortereau A., Milhau N., Rhumy E., Castets M., Ponce F., Mehlen P, & Marchal T. (2022). Implication of Netrin-1 Gain of Expression in Canine Nodal Lymphoma. Veterinary Sciences, 9(9), 494.

+ Open protocol

+ Expand

Splenic Dendritic Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were incubated with Fc block (clone 2.4G2) for 20 minutes on ice before surface staining with fluorescently labelled CD11c (clone N418), MHC-II (clone M5/114.15.2), CD4 (GK15), CD8 (clone 53–6.7), Vα2 TCR chain (clone B20.1) antibodies (all from eBioscience). For detection of EphB receptors and Ephrin molecules on splenic CD11c^hi DC, cells were incubated with recombinant mouse EphB2-Fc or mouse Ephrin B2-Fc chimeric proteins (R and D systems) at a concentration of 200ng/ml for 30 minutes on ice. Bound molecules were detected by incubation with a secondary biotinylated anti-human IgG Fc antibody (eBioscience) for 20 minutes followed by 15 minutes of incubation with APC-conjugated strepatavidin (eBioscience). T cell cytokines were detected by intracellular cytokine staining after fixation of cells in 2% paraformaldehyde solution and permeabilization using 0.5% saponin solution (Sigma).

Mimche P.N., Brady L.M., Keeton S., Fenne D.S., King T.P., Quicke K.M., Hudson L.E, & Lamb T.J. (2015). Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 10(9), e0138835.

+ Open protocol

+ Expand

Bacterial Cell Lysis and Suspension

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBC bottle was vortexed and 1 ml of PBC broth was collected. Two hundred microliters of 5% saponin solution (Sigma-Aldrich, St. Louis, USA) were added into the PBC broth, mixed thoroughly and incubated at room temperature for 5 mins. The tube was centrifuged at 13,000 rpm for 2 mins, and the supernatant was discarded. The pellet was washed with 1 ml-distilled water and centrifuged at 13,000 rpm for 1 min and the supernatant was discarded. The pellet was washed twice with 1 ml 0.45% sterile saline and the supernatant was discarded after centrifugation. The pellet was resuspended in 30–50 μl of 0.45% sterile saline. This suspension was used for preparing the inoculum for AST according to Vitek colony AST procedures. Ten microliters of the inoculum were inoculated onto SBA for purity check.

Sze D.T., Lau C.C., Chan T.M., Ma E.S, & Tang B.S. (2021). Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by Accelerate Pheno system and BioFire FilmArray Blood Culture Identification and BioFire FilmArray Blood Culture Identification 2 panels. BMC Microbiology, 21, 350.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!