Human HS11, HS01, HS02, HS13, and HS05 and mouse MTC-10 cells (1000 cells/well) were seeded in growth medium in three replicate wells/condition of a 384-well CellBIND Corning plate. After ~6 h, drugs (diluted in 0.001% DMSO) at three increasing concentrations (EC50 as well as lower and higher concentrations) and Incucyte® Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, Gottingen, Germany) were added to the wells. Wells were imaged using the Incucyte® S5 Live-Cell Analysis system (Sartorius) for 24–72 h. Phase and green fluorescent images were collected every 2 h and analyzed using the integrated basic analyzer software.
Incucyte s5 live cell analysis system
Manufactured by Sartorius
The Incucyte® S5 Live-Cell Analysis system is a real-time, automated cell imaging and analysis platform. It enables continuous monitoring and quantification of live-cell processes within a controlled, incubated environment.
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Lab products found in correlation
2 protocols using incucyte s5 live cell analysis system
1
Apoptosis Kinetics in Human and Mouse Cells
2
Apoptosis Monitoring of Cell Lines
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Human HS11, HS01, HS02, HS13 and HS05 and mouse MTC-10 cells (1,000 cells/well) were seeded in growth medium in three replicate wells/condition of a 384-well CellBIND Corning plate. After ~ 6h, drugs (diluted in 0.001% DMSO) at three increasing concentrations (EC50 as well as lower and higher concentrations) and Incucyte® Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, Gottingen, Germany) were added to the wells. Wells were imaged using the Incucyte® S5 Live-Cell Analysis system (Sartorius) for 24–72h. Phase and green fluorescent images were collected every 2h and analyzed using the integrated basic analyzer software.
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