HTR8/SVneo cells were transfected with si‐IGF‐1 or oe‐IGF‐1 plasmids, and the supernatant was collected and settled to a 96‐well plate containing 1.5 × 104 human uterine microvascular endothelial cells (HUtMEC; PromoCell), which were coated with matrix gel. After 16 h, the HUtMEC branch was observed under an optical microscope and photographed. The total number of branch points and branch tubes was manually calculated from photomicrography.
Hutmec
Manufactured by PromoCell
The HUtMEC is a primary human umbilical vein endothelial cell line. It is a type of lab equipment used for cell culture and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Endothelial cell growth medium mv, by PromoCell (2 mentions)
Egm tm 2 endometrial cell growth medium 2, by Lonza (2 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (2 mentions)
Dmem f12 media, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
4 protocols using hutmec
1
Evaluation of Angiogenic Potential
2
Effects of Botulinum Toxin A on Endothelial Cells
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Human umbilical vein cells (HUVEC, ATCC) and human uterine microvascular endothelial cells (HUtMEC, PromoCell) were maintained in EGMTM-2 endometrial cell growth medium 2 (Lonza) and endothelial cell growth medium MV (PromoCell), respectively. Ishikawa (ATCC) and CRL-4003 cells (kindly gifted from the laboratory of Dr. Haeng Seok Song) were maintained in DMEM/F12 media (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA) as previously described [27 (link)]. All experiments were conducted using cells of the passages between 1 and 10. For the analyses for the effects of BoTA, cells were treated with commercially available BoTA (Botulax, Hugel, Seoul, Korea) for 24h, 48h, or 72h at concentration of 0.5, 2.0, 10, or 20IU. Botulax is the type for Clostridium botulinum toxin A derived from the strain of Clostridium Botulinum CBFC26, and for the utilization of BoTA from Botulax (Hugel) to perform the all analyses, the powder form of BoTA was dissolved and diluted in sterile 0.9% saline solution.
3
Angiogenic Potential of dNK Cells
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MACS enriched CD56+ dNK cells were cultured with rhTGFb or SB. Conditioned supernatants were collected and added to 96-well plates coated with Matrigel containing 1.5 × 104 Human Uterine Microvascular Endothelial Cells (HUtMEC; PromoCell). After 16 h, HUtMEC branching was examined by light contrast microscopy and images were captured. Total number of branching points and tubes were counted manually from the photomicrography.
4
Endothelial Cell Culture and BoTA Treatment
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Cell culture and Botulinum Toxin A treatment.
Human Umbilical Vein Cells (HUVEC, ATCC) and Human Uterine Microvascular Endothelial Cells (HUtMEC, PromoCell) were maintained in EGM TM -2 endometrial cell growth medium 2 (Lonza) and Endothelial Cell Growth Medium MV (PromoCell) respectively. Ishikawa (ATCC) and CRL-4003 cells (kindly gifted from the laboratory of Dr. Haeng Seok Song) were maintained in DMEM/F12 media (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA) as previously described [28] . All experiments were conducted using cells of the passages between 1 and 10. For the analyses for the effects of BoTA, cells were treated with commercially available BoTA (Botulax, Hugel, Seoul, Korea) for 24h, 48h or 72h at concentration of 0.5, 2.0, 10, or 20IU. Botulax is the type for Clostridium Botulinum Toxin A derived from the strain of Clostridium Botulinum CBFC26 and for the utilization of BoTA from Botulax (Hugel) to perform the all analyses, the powder form of BoTA was dissolved and diluted in sterile 0.9% Saline solution.
Human Umbilical Vein Cells (HUVEC, ATCC) and Human Uterine Microvascular Endothelial Cells (HUtMEC, PromoCell) were maintained in EGM TM -2 endometrial cell growth medium 2 (Lonza) and Endothelial Cell Growth Medium MV (PromoCell) respectively. Ishikawa (ATCC) and CRL-4003 cells (kindly gifted from the laboratory of Dr. Haeng Seok Song) were maintained in DMEM/F12 media (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA) as previously described [28] . All experiments were conducted using cells of the passages between 1 and 10. For the analyses for the effects of BoTA, cells were treated with commercially available BoTA (Botulax, Hugel, Seoul, Korea) for 24h, 48h or 72h at concentration of 0.5, 2.0, 10, or 20IU. Botulax is the type for Clostridium Botulinum Toxin A derived from the strain of Clostridium Botulinum CBFC26 and for the utilization of BoTA from Botulax (Hugel) to perform the all analyses, the powder form of BoTA was dissolved and diluted in sterile 0.9% Saline solution.
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