For these studies, supernatants from PC3 cells treated with 5 ng rTRAIL were used. They were compared to the cytokine levels of untreated PC3 cells. The respective supernatants were diluted 10-fold and applied to human cytokine antibody arrays III (RayBio, Norcross, GA, USA) according to manufacturer’s instructions. Each cytokine signal was normalised to the internal control and the fold upregulation in response to rTRAIL was calculated.
Human cytokine antibody arrays 3
Manufactured by RayBiotech
Sourced in United States
The Human Cytokine Antibody Arrays III is a multiplex assay kit that allows for the simultaneous detection and quantification of multiple human cytokines, chemokines, and growth factors in a single experiment. The kit contains an array of capture antibodies spotted onto a membrane, enabling the parallel measurement of a panel of analytes from a small sample volume.
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Lab products found in correlation
2 protocols using human cytokine antibody arrays 3
1
Cytokine Profiling of rTRAIL-Treated PC3 Cells
2
Cytokine Profiling of MSC-Tumor Co-Culture
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Supernatants were taken from hMSCs and tumour cells at a ratio of one MSC to ten tumour cells and cytokine levels were compared to the MSCs and tumour cells cultured separately.
The supernatants were diluted 3.5-fold and applied to human cytokine antibody arrays III (RayBiotech, Norcross, GA, USA) according to the manufacturer's instructions. Each cytokine signal was normalised to its internal control and the fold change calculated.
The supernatants were diluted 3.5-fold and applied to human cytokine antibody arrays III (RayBiotech, Norcross, GA, USA) according to the manufacturer's instructions. Each cytokine signal was normalised to its internal control and the fold change calculated.
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