Structured illumination microscopy (SIM) of PLK4–GFP assemblies was performed on an N-SIM system, Nikon Inc., equipped with Apo TIRF 100× NA 1.49 Plan Apo oil objective, back-illuminated EMCCD camera (Andor, DU897), and 405, 488, 561 and 640 nm excitation lasers. 100-nm z sections were acquired in 3D SIM mode generating 15 images per plan, and reconstructed. xyz corrections were applied using the signals of 100 nm multi-spectral fluorescent spheres (Invitrogen) included in the sample.
Apo tirf 100 na 1.49 plan apo oil objective
Manufactured by Oxford Instruments
The Apo TIRF 100× NA 1.49 Plan Apo oil objective is a high-performance microscope objective designed for total internal reflection fluorescence (TIRF) microscopy. It has a numerical aperture of 1.49, which enables the collection of a large amount of fluorescence signal from the sample. The objective is plan-apochromatic, ensuring high image quality and resolution across the field of view. It is optimized for use with oil immersion, which further enhances the optical performance.
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Lab products found in correlation
4 protocols using apo tirf 100 na 1.49 plan apo oil objective
1
Imaging PLK4 Assemblies by Structured Illumination
2
3D SIM Imaging of Microscopic Samples
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SIM was performed on N-SIM, Nikon Inc., equipped with 405, 488, 561, and 640 nm excitation lasers, Apo TIRF 100× NA 1.49 Plan Apo oil objective, and back-illuminated 16 µm pixel EMCCD camera (DU897; Andor). 100-nm-thick Z sections were acquired in 3D SIM mode and reconstructed.
3
3D-SIM Microscopy Imaging Protocol
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SIM was performed using N-SIM (Nikon), equipped with 405-, 488-, 561-, and 640-nm excitation lasers, Apo TIRF 100× NA 1.49 Plan Apo oil objective, and a back-illuminated 16 µm pixel electron multiplying CCD camera (Andor; DU897). 100-nm-thick Z-sections were acquired in 3D SIM mode and reconstructed to generate a final image using Nikon NIS-Elements software.
4
Super-resolution 3D imaging protocol
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SIM was performed using N‐SIM, Nikon Inc., equipped with 405, 488, 561 and 640 nm excitation lasers, Apo TIRF 100× NA 1.49 Plan Apo oil objective and back‐illuminated 16 µm pixel EMCCD camera (Andor; DU897). Hundred nanometre thick Z‐sections were acquired in 3D SIM mode and reconstructed to generate a final image using Nikon NIS‐Elements software.
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