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4 protocols using horseradish peroxidase

1

Sialylglycopolymer Binding Assay

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Fetuin and horseradish peroxidase were from Serva, Switzerland. Antibodies against mouse and chicken immunoglobulins conjugated with horseradish peroxidase were from Sigma-Aldrich, Saint Louis, MA, USA. Sialylglycopolymers were from GlycoNZ (Auckland, New Zealand). MDCK cells, American Type Culture Collection number ATCC CCL-34, were kindly provided by Mikhail Matrosovich at the Institute of Virology, Germany.
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2

Glycopolymer-based Sialic Acid Study

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Fetuin and horseradish peroxidase were from Serva, Switzerland. Antibodies against mouse and chicken immunoglobulins conjugated with horseradish peroxidase were from Sigma-Aldrich, Inc., St. Louis, MO, USA. MycoKill AB solutions were from PAA Laboratories GmbH, Pasching, Austria. Viral RNA Mini Kit was from QIAGEN, Hilden, Germany. MMLV Reverse Transcription kit, random primers, nuclease-free water, DNA Ladder, and TAE buffer were from Evrogen, Moscow, Russia. Ribonuclease inhibitors were from Syntol, Moscow, Russia. Soluble synthetic polyN-(2-hydroxyethyl)acrylamide-based sialylglycopolymers (SGP) contained 20 mol% of specific sialyloligosaccharide attached to the 30-kDa polymer were from GlycoNZ, Auckland, New Zealand.
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3

Serological Assay of Viral Proteins

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Fetuin and horseradish peroxidase were from Serva, Oftringen, Switzerland. Antibodies against mouse immunoglobulins conjugated with horseradish peroxidase were from Sigma, St. Louis, MO, USA. Viral RNA Mini Kit was from QIAGEN, Hilden, Germany. MycoKill AB solution was from PAA Laboratories GmbH, Pasching, Austria. Ethidium Bromide Solution was from Promega, Madison, WI, USA. MMLV Reverse Transcription kit, random primers, nuclease free water, DNA Ladder, and TAE buffer were from Evrogen, Moscow, Russia. Ribonuclease Inhibitor was from Syntol, Moscow, Russia. Embryonated chicken eggs were purchased from the State poultry farm “Ptichnoe” (Moscow, Russia).
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4

Hematopoietic Cytokine Signaling Assay

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Luminol (11050, AAT-Bioquest), horseradish peroxidase (31941, Serva), PMA (P8139, Sigma), A23187 (Santa Cruz Biotechnology Inc), GATA-1 inhibitor (anagrelide, SML0846, Sigma), GATA-2 inhibitor (K-7174, HY-12743A, MedChemExpress) were used. Recombinant murine cytokines (IL-3, IL-5, IL-9, GM-CSF, SCF, G-CSF) were from Peprotech.
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