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2 protocols using sc 22831

1

Immunofluorescence and Western Blotting Antibodies

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The following antibodies were used for immunofluorescence. rabbit anti- γH2AX (ab11174, Abcam); mouse anti-γH2AX (ab26350, Abcam); rabbit anti-c-Kit (3074S, Cell Signaling Technology); rabbit anti-GFRα1; (AF560-SP, R and D Systems); rabbit anti-Ki67 (ab15580, Abcam); rabbit anti-MPC1 (HPA045119, Millipore Sigma); mouse anti-MTCOI (ab14705, Abcam); mouse anti-Ndufb6 (ab110244, Abcam); rabbit anti-PLZF (SC-22831, Santa Cruz Biotech); mouse anti-PLZF (SC-28319, Santa Cruz Biotech); rabbit anti-SCP3; (ab15093, Abcam); mouse anti-SCP3 (ab97672, Abcam); mouse anti-SDHA (ab14715, Abcam); guinea pig anti-SP-10 (Gift from Prabhakara P. Reddi); rabbit anti-Tom20 (SC-11415, Santa Cruz, Biotech); mouse anti-Total OXPHOS (ab110413, Abcam). The following antibodies were used for western blotting. Chicken anti-Mfn1 (Chen et al., 2003 (link)); rabbit anti-Mfn2 (D2D10S #9482, Cell Signaling Technology); mouse anti-tubulin (T6199, Sigma).
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2

Sulforaphane-Mediated Regulation of NRF2 Pathway

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Sulforaphane (S6317), as well as anti-mouse IgG (HRP) (1:3000, A-9044) and anti-rabbit IgG (HRP) (1:3000, A-0545) antibodies were purchased from Sigma-Aldrich. LY294002 (ab120243) was purchased from Abcam. The rabbit normal IgG and antibodies against NRF2 (1:1000, sc-13032), sMAF (1:1000, sc-22831), NQO1 (1:1000, sc-32793), FN1 (1:1000, sc-8422), COL1A1 (1:1000, sc-293,182), α-SMA (1:1000, sc-53142), N-Cadherin (1:500, sc-7939) and GAPDH (1:3000, sc-32233) were purchased from Santa Cruz Biotechnology. The antibody against E-Cadherin (1:1000, 610,181) was purchased from BD Biosciences. Antibodies against AKT (1:2000, 4691S) and phospho-AKT (Ser473) (1:1000, 4060S) were purchased from Cell Signaling Technology. TGF-β (100–21) was purchased from PeproTech.
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