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Pzsgreen n1

Manufactured by Takara Bio
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The PZsGreen-N1 is a fluorescent protein-based reporter system developed by Takara Bio. It is designed to express the green fluorescent protein (GFP) variant, ZsGreen, as a fusion protein with an N-terminal tag. The core function of the PZsGreen-N1 is to provide a tool for tracking and visualizing the expression of target proteins in living cells.

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Lab products found in correlation

Purelink hipure plasmid maxiprep kit, by Thermo Fisher Scientific (2 mentions) Nanodrop onec uv vis, by Thermo Fisher Scientific (2 mentions) Lb medium, by Merck Group (1 mentions)

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PZsGreen (2 citations)

2 protocols using pzsgreen n1

1

Purification of pZsGreen-N1 Plasmid

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E. coli Stbl3 transformed with pZsGreen-N1 (Clontech laboratories) that encodes ZsGreen, a green fluorescent protein (GFP), were grown in LB medium and the plasmid was purified by PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen), following the manufacturer’s instructions and quantified by NanoDrop-OneC-UV-Vis (Thermo Fisher).
Scalzo S., Santos A.K., Ferreira H.A., Costa P.A., Prazeres P.H., da Silva N.J., Guimarães L.C., e Silva M.D., Rodrigues Alves M.T., Viana C.T., Jesus I.C., Rodrigues A.P., Birbrair A., Lobo A.O., Frezard F., Mitchell M.J., Guatimosim S, & Guimaraes P.P. (2022). Ionizable Lipid Nanoparticle-Mediated Delivery of Plasmid DNA in Cardiomyocytes. International Journal of Nanomedicine, 17, 2865-2881.
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2

Bacterial Plasmid Preparation and Quantification

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E. coli Stbl3 transformed with pZsGreen-N1 (Clontech laboratories, no. 632448) encoding ZsGreen, a green fluorescent protein (GFP), or recombinant HexaPro Spike plasmid (Addgene, no. 154754) (Supplementary Fig. 2) were grown in LB medium (Sigma-Aldrich, no. L3022) containing kanamycin (50 μg/ml) or ampicillin (100 μg/mL), respectively, at 37 °C for 16 h. The plasmids were purified by PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen, no. K210007), following the manufacturer’s instructions, and quantified using a NanoDrop-OneC-UV-Vis (ThermoFisher, no. ND-ONE-W).
Guimaraes L.C., Costa P.A., Scalzo Júnior S.R., Ferreira H.A., Braga A.C., de Oliveira L.C., Figueiredo M.M., Shepherd S., Hamilton A., Queiroz-Junior C.M., da Silva W.N., da Silva N.J., Rodrigues Alves M.T., Santos A.K., de Faria K.K., Marim F.M., Fukumasu H., Birbrair A., Teixeira-Carvalho A., de Aguiar R.S., Mitchell M.J., Teixeira M.M., Vasconcelos Costa V., Frezard F, & Guimaraes P.P. (2024). Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models. Nature Communications, 15, 590.
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