MultiScreen 96-well Filtration plates (Millipore) were coated with 20 μg ml−1 NP5-BSA or NP25-BSA. Splenocytes or bone marrow cells (1–5×105/well) were then added to the plates and incubated at 37 °C for 5 h. The cells were lysed with H2O and the wells were probed with HRP-conjugated goat anti-mouse IgG1 or anti-mouse IgM (Southern Biotechnology), followed by development with 3-amino-9-ethylcarbzole (Sigma). To detect HA-specific antibody forming cells, the MultiScreen Filtration plates were coated with 10 μg ml−1 H3N2 HA (Sino Biological). Splenocytes or lung cells (1–5×105/well) were then added to the plates and incubated at 37 °C for 5 h. The cells were lysed with H2O and the wells were probed with HRP-conjugated goat anti-mouse IgG or anti-mouse IgA (Southern Biotechnology) and developed as above.
Hrp conjugated goat anti mouse igg1 or anti mouse igm
Manufactured by Southern Biotech
HRP-conjugated goat anti-mouse IgG1 or anti-mouse IgM is a laboratory reagent used for the detection and quantification of mouse IgG1 or IgM antibodies in various immunoassays. It consists of goat-derived antibodies that are conjugated to the horseradish peroxidase (HRP) enzyme, which can be used as a detection system.
Automatically generated - may contain errors
2 protocols using hrp conjugated goat anti mouse igg1 or anti mouse igm
1
Antibody-forming Cell Detection Assay
2
Antibody-forming Cell Detection Assay
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MultiScreen 96-well Filtration plates (Millipore) were coated with 20 μg ml−1 NP5-BSA or NP25-BSA. Splenocytes or bone marrow cells (1–5×105/well) were then added to the plates and incubated at 37 °C for 5 h. The cells were lysed with H2O and the wells were probed with HRP-conjugated goat anti-mouse IgG1 or anti-mouse IgM (Southern Biotechnology), followed by development with 3-amino-9-ethylcarbzole (Sigma). To detect HA-specific antibody forming cells, the MultiScreen Filtration plates were coated with 10 μg ml−1 H3N2 HA (Sino Biological). Splenocytes or lung cells (1–5×105/well) were then added to the plates and incubated at 37 °C for 5 h. The cells were lysed with H2O and the wells were probed with HRP-conjugated goat anti-mouse IgG or anti-mouse IgA (Southern Biotechnology) and developed as above.
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