Antibodies against atf3

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Antibodies against ATF3 are designed to detect and identify the ATF3 (Activating Transcription Factor 3) protein. ATF3 is a transcription factor that plays a role in cellular stress response. These antibodies can be used in various research applications, such as western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of ATF3 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

β actin, by Santa Cruz Biotechnology (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Baicalein, by Merck Group (1 mentions) Cobalt protoporphyrin 9 copp, by Merck Group (1 mentions) Zinc protoporphyrin 9 znpp, by Merck Group (1 mentions) Rna extraction kit, by iNtRON Biotechnology (1 mentions) Tnf α, by Santa Cruz Biotechnology (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions) C jun, by Santa Cruz Biotechnology (1 mentions) Cell culture medium, by Thermo Fisher Scientific (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

3 protocols using antibodies against atf3

Antioxidant Modulation of ER Stress

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RPMI 1640 medium, fetal bovine serum (FBS), fetal calf serum (FCS), penicillin, and streptomycin were obtained from GIBCO (Grand Island, NY). Antibodies against ATF-3, CHOP, GRP78, HO-1, ERK, pERK, and β-actin were from Santa Cruz Biotechnology (Santa Cruz, CA). The RNA extraction kit was from Intron Biotechnology (Seoul, Korea). Rat oligonucleotide primers for ATF-3, GRP78, CHOP, HO-1, GAPDH, and rat HO-1 siRNA were from Bioneer Co. Ltd (Daejeon, Korea). PD98059 was from Calbiochem-Novabiochem Co. (San Diego, CA). Baicalein, tricarbonyldichloro-ruthenium(II) dimer (RuCO), cobalt protoporphyrin IX (CoPP), zinc protoporphyrin IX (ZnPP) and all other chemicals were from Sigma-Aldrich (St Louis, MO).

Kwak H.J., Yang D., Hwang Y., Jun H.S, & Cheon H.G. (2017). Baicalein protects rat insulinoma INS-1 cells from palmitate-induced lipotoxicity by inducing HO-1. PLoS ONE, 12(4), e0176432.

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Quantifying Protein Expression Levels

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Antibodies against ATF3, TLR4, TNF-α, c-Jun, and β-actin were purchased from Santa Cruz Biotechnologies, and the antibodies for p-JNK, JNK, p38, p-p38, ERK, and p-ERK were purchased from Cell Signaling Technology (USA). The concentrations of the protein samples were estimated using the DC™ protein assay kit (Bio-rad, USA). Protein samples (25–30 μg each) were separated on a 7–15% (wt/vol) polyacrylamide gel (SDS-PAGE) and transferred onto polyvinyli-denedifluoride (PVDF) membranes (Millipore, USA) for Western blotting, as previously described (Nguyen et al., 2014b (link)).

Nguyen C.T., Kim E.H., Luong T.T., Pyo S, & Rhee D.K. (2014). TLR4 Mediates Pneumolysin-Induced ATF3 Expression through the JNK/p38 Pathway in Streptococcus pneumoniae-Infected RAW 264.7 Cells. Molecules and Cells, 38(1), 58-64.

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Transcription Factor and Reporter Assay

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Both cell culture reagents and cell culture medium were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against ATF3, FOXP3, and β-Actin (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). Luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA).

Wang C.M., Yang W.H., Cardoso L., Gutierrez N., Yang R.H, & Yang W.H. (2021). Forkhead Box Protein P3 (FOXP3) Represses ATF3 Transcriptional Activity. International Journal of Molecular Sciences, 22(21), 11400.

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