Fibronectin 610077

Manufactured by BD

Sourced in United States

Fibronectin (610077) is a cellular adhesion protein that promotes cell attachment and migration. It is a high molecular weight glycoprotein found in the extracellular matrix and on the surface of many cell types. Fibronectin plays a critical role in cell-to-cell and cell-to-matrix interactions.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Fibronectin (329 citations)

5 protocols using fibronectin 610077

Antibody Generation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-DDX3 antibodies were generated as described previously34 (link)35 (link). Antibodies against Nanog (#3580), Oct4 (#2840), c-Myc (#9402), Bmi1 (#2830) and CK19 (#4558) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Sox2 (GTX627404), anti-KLF4 (GTX101508), anti-DNMT3A (GTX116011), anti-DNMT3B (GTX62171) and anti-DNMT1 (GTX62550) antibodies were purchased from GeneTex (Irvine, CA, USA). Anti-β-actin antibodies were purchased from Sigma. Antibodies against E-cadherin (610181) and fibronectin (610077) were purchased from BD biosciences (Franklin Lakes, NJ, USA). Phycoerythrin (PE)-conjugated anti-CD133 (130-090-853), anti-CD13 (130-103-733), anti-EpCAM (130-091-253) and anti-CD90 (130-095-400) antibodies were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). H3K27me3 antibody (ABE44) was purchased from Millipore (Cambridge, MA, USA).

Li H.K., Mai R.T., Huang H.D., Chou C.H., Chang Y.A., Chang Y.W., You L.R., Chen C.M, & Lee Y.H. (2016). DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma. Scientific Reports, 6, 28637.

+ Open protocol

+ Expand

Immunofluorescence and Cell Assays Protocol

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study: SHROOM2 (SAB2700199), Vinculin (V9131) and Phalloidin–Tetramethylrhodamine B isothiocyanate (P1951) were from Sigma; E-Cadherin (24E10), N-Cadherin (13116), MLC2 (3672), p-MLC2 (T18/S19) (3674), HA (3724) were from Cell Signaling Technology; ROCK1 (ab58305), CK5 (ab52635), CK10 (ab76318), Desmoglein1 (ab12077) and RAC1 (ab155938) were from abcam; p-MYPT (ABS45) was from Millipore and Fibronectin (610077) was from BD biosciences. Horseradish peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies were purchased from Promega.
Immunofluorescence microscopy, cell proliferation, colony formation, soft agar assays were performed as described previously^{8 (link),42 (link)}.

Yuan J., Chen L., Xiao J., Qi X.K., Zhang J., Li X., Wang Z., Lian Y.F., Xiang T., Zhang Y., Chen M.Y., Bei J.X., Zeng Y.X, & Feng L. (2019). SHROOM2 inhibits tumor metastasis through RhoA–ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death & Disease, 10(2), 58.

+ Open protocol

+ Expand

Immunoblotting and Immunofluorescence Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

L-glucose, D-glucose, and streptozocin were purchased from Sigma (St. Louis, MO, USA). α-SMA (A2547), β-actin (A5411), and Twist (T6451) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Antibodies against Vimentin (550513), N-cadherin (610920), and fibronectin (610077) were obtained from BD (Franklin Lakes, NJ, USA). Antibodies for Snail (3879S), β-catenin (9582S), and Glial Fibrillary Acidic Protein (GFAP, 3670S) were from Cell Signaling Technology (Danvers, MA, USA). CTGF (ab6922) and Glutamine synthase (GS, ab176562) antibodies were purchased from Abcam (Cambridge, MA, USA). Glutamine synthase (GS, MAB302) antibody was from Millipore (Billerica, MA, USA). Goat Anti-mouse(PI-2000) and anti-rabbit (PI-1000) horseradish peroxidase (HRP)-conjugated secondary antibodies were from Vector Laboratories (Burlingame, CA, USA). Alexa Flour 488 goat anti-rabbit/anti-mouse (A21206/A21202) and Alexa Fluor 594 goat anti-rabbit/anti-mouse (A21207/A21203) antibodies and 4’,6-Diamidino-2-phenylindole (DAPI, D1306) were from Life Technology (St. Louis, MO, USA).

Zhou T., Che D., Lan Y., Fang Z., Xie J., Gong H., Li C., Feng J., Hong H., Qi W., Ma C., Yang Z., Cai W., Zhong J., Ma J., Yang X, & Gao G. (2016). Mesenchymal marker expression is elevated in Müller cells exposed to high glucose and in animal models of diabetic retinopathy. Oncotarget, 8(3), 4582-4594.

+ Open protocol

+ Expand

Fibrosis-related Protein Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

PAI-1 (614024) and fibronectin (610077) antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). Phospho-Smad3 (#9520) and Smad2/3 (#3102) antibodies were provided by Cell Signaling (Danvers, MA, USA). CTGF antibodies (SC-101586) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and TGF-β1 antibody (NBP1-80289) was obtained from Novus Biologicals (Littleton, CO). Horseradish peroxidase-conjugated goat anti-rabbit (G21234) and anti-mouse (G21040) antibodies were purchased from Invitrogen (Carlsbad, CA, USA). α-SMA (A2547) and β-actin (A5441) antibodies, MTT (M2128), and dimethylsulfoxide (D8418) were acquired from Sigma Chemicals (St. Louis, MO, USA). TGF-β (240-B) was purchased from R&D Systems (Minneapolis, MN, USA). CTGF antibody (sc-101586) obtained from Santa Cruz Biotechnology (Santa Cruz, CA). CCl₄ (33650-0330) was received from Junsei Chemical (Kyoto, CO).

Yang J.H., Ku S.K., Cho I.J., Lee J.H., Na C.S, & Ki S.H. (2021). Neoagarooligosaccharide Protects against Hepatic Fibrosis via Inhibition of TGF-β/Smad Signaling Pathway. International Journal of Molecular Sciences, 22(4), 2041.

+ Open protocol

+ Expand

FAK Inhibitor's Impact on Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The BRACs used in this study were purchased from the New Star Natural Plant Development Company (Jilin, China). The FAK inhibitor, 1,2,4,5-benzenetetramine tetrahydrochloride (Y15), was obtained from Sigma (St. Louis, MO, USA). Fetal bovine serum (FBS) and horse serum were purchased from Gibco-BRL (Grand Island, NY, USA). Dulbecco's modified Eagle's medium (DMEM)/high glucose and DMEM/F12 were purchased from HyClone (Beijing, China). L15 medium was obtained from Keygen Biotech (Nanjing, China). Primary antibodies against FAK (ab40794), phospho-FAK (ab38458, ab81298), Src (ab16885), phospho-Src (ab24789), and p130^Cas (ab136514) were purchased from Abcam (Cambridge, MA, USA). Phospho-p130^Cas (#4011) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against E-cadherin (610181), vimentin (550513), and fibronectin (610077) were purchased from BD Biosciences (Bedford, MA, USA).

Zhou J., Zhu Y.F., Chen X.Y., Han B., Li F., Chen J.Y., Peng X.L., Luo L.P., Chen W, & Yu X.P. (2017). Black rice-derived anthocyanins inhibit HER-2-positive breast cancer epithelial-mesenchymal transition-mediated metastasis in vitro by suppressing FAK signaling. International Journal of Molecular Medicine, 40(6), 1649-1656.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!