Interleukin 6 (il 6)

The serum levels of IL17A (Invitrogen, Carls, CA, USA), IL6 and IL2 (KomaBiotech, Seoul, Korea) were measured according to manufacturer’s instructions.

High glucose Dulbecco's modified Eagle's medium (DMEM) was obtained from Welgene Inc. (Gyeongsangbuk, Korea), and Iscove's modified Dulbecco's medium (IMDM) acquired from Merck Millipore (Darmstadt, Germany). Fetal bovine serum (FBS) and antibiotics were purchased from Invitrogen Inc. (Carlsbad, CA, USA). 2,4-Dinitrochlorobenzene (DNCB), corticotropin-releasing hormone (CRH), substance P (SP), 1-thioglycerol, and dexamethasone (DEX) were procured from Sigma-Aldrich (St. Louis, MO, USA). Human VEGF and mouse IFN-γ, IL-1β, IL-6, IL-13, TNF-α, VEGF, eotaxin, and IgE ELISA kits were supplied by Koma Biotech Inc. (Seoul, Korea). Human MDC, TARC ELISA kits were obtained from R&D Systems (Minneapolis, MN, USA). The mouse CORT ELISA kits were purchased from Arigo Biolaboratories (Hsinchu, Taiwan), and Mouse CRH and ACTH ELISA kits were procured from LifeSpan BioSciences (Seattle, WA, USA). Antibodies for phospho-p38, phospho-JNK, phospho-ERK, phospho-STAT1, phospho-I-κB-α, p38, JNK, ERK, STAT1, I-κB-α, and NF-κB were acquired from Cell Signaling Technology (Danvers, MA, USA). Antibodies against loricrin, involucrin, and lamin B2 were supplied by Abcam (Cambridge, MA, USA). HRP-conjugated anti-β-actin was obtained from Sigma-Aldrich (St. Louis, MO, USA).