The antibody response to SARS-CoV-2 S protein was measured using the Elecsys® Anti-SARS-CoV-2 S test (quantitative), while the antibody response to SARS-CoV-2 N protein was measured using the Elecsys® Anti-SARS-CoV-2 IgM/IgA/IgG test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland, semi-quantitative) according to the manufacturer’s instructions. A lack of response to the N-protein was defined at anti-N protein antibody titers lower than one.
Elecsys anti sars cov 2 s test
Manufactured by Roche
Sourced in Switzerland
The Elecsys® Anti-SARS-CoV-2 S test is a lab equipment product developed by Roche. It is an immunoassay used for the quantitative determination of antibodies to the SARS-CoV-2 spike (S) protein in human serum and plasma.
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6 protocols using elecsys anti sars cov 2 s test
1
SARS-CoV-2 Antibody Response Measurement
2
Quantifying SARS-CoV-2 Antibody Responses
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Antibody response to SARS-CoV-2 S protein was measured using the Elecsys® Anti-SARS-CoV-2 S test (quantitative), while antibody response to SARS-CoV-2 N protein was measured using the Elecsys® Anti-SARS-CoV-2 IgM/IgA/IgG test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland, semiquantitative) according to the manufacturer's instructions. Lack of response to the N protein was defined at anti-N protein antibody titers lower than one. The titers of anti-S protein antibodies have an upper limit of 25,000 BAU/mL since the last dilution performed in the routine analysis is 1/100; samples with titers higher than 25,000 BAU/mL have been assigned 25,000 BAU/mL.
3
Serological Assay for SARS-CoV-2 Antibodies
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The Elecsys Anti-SARS-CoV-2 S test (Roche Diagnostics GmbH) [14 ] detecting complete Ig directed to Spike-(S) protein receptor-binding-domain (RBD) was operated on the COBAS pro e 801 module according to the manufacturer’s recommendations. Cutoff values were chosen as specified by the manufacturer. Test characteristics are shown in Table 1 .
4
Quantitative Antibody Detection in SARS-CoV-2
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Serum samples were analyzed for detection of antibodies to SARS-CoV-2 spike using the quantitative Elecsys® Anti-SARS-CoV-2 S test (Roche Diagnostics) (25 (link)) on the Cobas 8000 e801pro. The measuring range was between 0.40 to 250 U/mL, and the cut-off value for positive results is ≥ 0.80 U/mL Positive samples with antibody titers of >250 U/mL were re-tested following 1/10 dilution, and in some cases 1/100 dilution with the upper level of measuring range 25,000 U/mL.
5
Serological Assessment of SARS-CoV-2 Antibodies
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Elecsys Anti-SARS-CoV-2 S test (Roche Diagnostics, Rotkreuz, Switzerland) was used to test for SARS-CoV-2 IgG/total antibodies. This immunoassay targets antibodies against the viral spikes protein, specifically its receptor-binding domain (RBD), a common target for virus-neutralizing antibodies and the focus of therapeutics and vaccine design. After the 5 ml of blood was obtained from each participant, it was placed into serum separator tubes and allowed to coagulate at room temperature for 60 min. It was then stored at 4 °C until centrifugation. The serum was then analyzed according to the manufacturer’s recommendations.
6
Antibody Response to SARS-CoV-2 Vaccines in Rheumatic Diseases
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We collected various demographic and clinical information: from the subjects, including age, sex, type of vaccine received (BNT162b2 or mRNA-1237), dates of vaccination (second and third doses), dates of sample extraction, specific rheumatic disease, treatment regimen for the disease, and information on current glucocorticoid use as a binary variable.
Antibodies against S and N SARS-CoV-2 protein
Antibody response to SARS-CoV-2 S protein was measured using the Elecsys® Anti-SARS-CoV-2 S test (Roche Diagnostics International Ltd, Rotkreuz, Switzerland, quantitative) according to manufacture instructions. In these experiments, the standards and international units proposed by the WHO for the determination of antibodies against SARS-CoV-2 (Binding Antibody Units, BAU/ml) [22 (link)] were used. Based on previous studies [23 (link)], a value of 260 has been established as the minimum to consider the presence of a protective level against SARS-CoV-2 and, therefore, the minimum value to consider a patient as a responder to the vaccine.
Antibodies against S and N SARS-CoV-2 protein
Antibody response to SARS-CoV-2 S protein was measured using the Elecsys® Anti-SARS-CoV-2 S test (Roche Diagnostics International Ltd, Rotkreuz, Switzerland, quantitative) according to manufacture instructions. In these experiments, the standards and international units proposed by the WHO for the determination of antibodies against SARS-CoV-2 (Binding Antibody Units, BAU/ml) [22 (link)] were used. Based on previous studies [23 (link)], a value of 260 has been established as the minimum to consider the presence of a protective level against SARS-CoV-2 and, therefore, the minimum value to consider a patient as a responder to the vaccine.
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