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Agilent pl gpc50

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The Agilent PL-GPC50 is a gel permeation chromatography (GPC) system designed for the analysis of molecular weight and molecular weight distribution of polymers. It is a highly accurate and reliable instrument that provides precise measurements of polymer properties.

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Lab products found in correlation

Ultrahydrogel linear column, by Waters Corporation (1 mentions) Cary 5000 uv vis nir spectrometer, by Agilent Technologies (1 mentions) Cary 60 spectrometer, by Agilent Technologies (1 mentions) Hexafluoroisopropanol, by Merck Group (1 mentions)

Spelling variants of this product

PL-GPC50 (29 citations) PL-GPC50/Agilent 1260 (2 citations)

4 protocols using agilent pl gpc50

1

Determining Polysaccharide Molecular Weight

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The average molecular weight (Mw) of
crude polysaccharides is determined using an Agilent PL-GPC 50 (Agilent
Technologies, Amstelveen, Netherlands) apparatus equipped with a refractive
index detector (RID, G1362A) and an ultrahydrogel linear column (300
mm × 7.8 mm i.d., Waters Corporation, MA, USA).32 (link) The sample solution is prepared by dissolving 1.0 mg of
polysaccharides in 1.0 mL of purified water. After being treated with
a 0.22 μm membrane filter, 20 μL of sample solution is
injected into a chromatographic system. The mobile phase is 0.02 M
KH2PO4 (pH 6.0), the flow rate is 0.6 mL/min,
the column temperature is 35 °C, and the detector temperature
is 45 °C.
Agilent GPC software A.02.01 is applied to process
data. The relationship between Mw and
the elution volume is built according to a previously reported study.33 (link) Preliminary calibration is conducted using dextrans
with various molecular weights (5, 12, 50, 150, 210, and 410 kDa)
as references. The Mw of the sample is
estimated by the calibration equation established above.
Liu S., Chen L., Duan W., Meng Z., Dong H, & Wang X. (2022). Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products. ACS Omega, 7(50), 46833-46842.
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2

Characterization of Fulvic Acid Structure

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Cary 5000 UV-Vis-NIR spectrometer (Agilent) was used to collect the diffuse reflectance UV-visible (DR-UV-vis) spectra of the solid samples. Cary 60 spectrometer (Varian) was used to collect absorption spectra for liquid samples. The emission and excitation spectra were recorded on a Quanta Master 4 fluorescence spectrometer using quartz cuvettes loaded with 2 mL of sample
The molecular weight distribution of fulvic acid in DMSO was analyzed with Gel permeation chromatography (Agilent PL-GPC50, Agilent) using a PLgel Olexis column (300 mm × 7.5 mm, Agilent). The mobile phase was DMSO at a flow rate of 1 mL/min, 100 μL injection volume, and molecular weights were determined by comparison with Pullulan standards.
Tana T., Han P., Brock A.J., Mao X., Sarina S., Waclawik E.R., Du A., Bottle S.E, & Zhu H.Y. (2023). Photocatalytic conversion of sugars to 5-hydroxymethylfurfural using aluminium(III) and fulvic acid. Nature Communications, 14, 4609.
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3

Molecular Distribution Analysis by GPC

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The molecular distribution of synthetic products was determined through gel permeation chromatography (Agilent PL-GPC50, Agilent Technologies, Santa Clara, CA, USA). The test parameters were as follows: the concentration of the test sample was 0.10 mg/mL, the injection volume was 100 microliters, and the distribution coefficient was 14.1000. This describes the parameters of the distribution process or adsorption–desorption process of the components in the fixed and mobile phases.
Zheng Q., Nong G, & Li N. (2024). Preparation and Structural Analysis of a Water-Soluble Aminated Lignin. Polymers, 16(9), 1237.
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4

Measuring PA6 Molecular Properties

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The molecular weight and polydispersity index of PA6 samples were measured by GPC instrument (Agilent PL-GPC50, Agilent Technologies Inc., Palo Alto, CA, USA). A calibration curve was obtained using linear polystyrene (Agilent Technologies Inc., Palo Alto, CA, USA) as a standard and chromatographic grade hexafluoroisopropanol (Sigma-Aldrich, St. Louis, MO, USA) as the mobile phase at an elution rate of 1 mL/min (37 °C). The curves were normalized.
Tian S., Zhang J., Zhou Q., Shi L., Wang W, & Wang D. (2021). Photochromic Polyamide 6 Based on Spiropyran Synthesized via Hydrolyzed Ring-Opening Polymerization. Polymers, 13(15), 2496.
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