Prime script trimester mix

Total RNA was extracted from lung tissue samples or cells with TRIzol. Prime ScriptTM Trimester Mix (Takara, Japan) was utilized for reverse transcription of RNA. SYBR Premix Ex TaqTM11 (Takara, Japan) and a Step One Plus RT-PCR system (Life Technologies, USA) were utilized to perform RT-PCR based on the manufacturer’s instructions. The results were normalized to those for β-actin, and quantification was performed with the 2^{-ΔΔCt of each sample}/2^{-ΔΔCt of Ctrl} method. The primers are shown in Table S3.

Total RNA from MSCs, MSC-NC, and MSC-TGF-β1 was extracted using TRIzol reagent (Life Technologies, USA). RNA was reverse transcribed using Prime Script TM Trimester Mix (Takara, Japan) and subjected to RT-PCR with SYBR Premix Ex TaqTM11 (Takara, Japan) and a Step One Plus RT-PCR system (Life Technologies, USA). β-Actin was used as an endogenous control. RT-PCR primers were synthesized by Sangon Biotech (Shanghai, China) and the sequences were as follows: mouse β-actin forward primer, 5′-GGGAAATCGTGCGTGAC-3′, and reverse primer, 5′-AGGCTGGAAAAGAGCCT-3′, and mouse TGF-β1 forward primer, 5′-AGACGGAATACAGGGCTTTCGATTCA-3′, and reverse primer, 5′-CTTGGGCTTGCGACCCACGTAGTA-3′.

Total RNA from RAW264.7 cells was extracted using TRIzol reagent (Life Technologies, USA). Reverse transcription of RNA and RT-PCR was performed by using Prime Script TM Trimester Mix (Takara, Japan) and SYBR Premix Ex TaqTM11 (Takara, Japan) with a Step One Plus RT-PCR system (Life Technologies, USA) according to the manufacturer’s instructions. β-actin was used as an endogenous control. Primers for RT-PCR were synthesized by Sangon Biotech (Shanghai, China). The primer sequences are as follows: mouse b-actin forward primer, 5′-GGGAAATCGTGCGTGAC-3′ and
reverse primer, 5′-AGGCTGGAAAAGAGCCT-3′;
mouse ARG-1 forward primer, 5′-AACACTCCCCTGACAACCA-3′ and
reverse primer, 5′-CATCACCTTGCCAATCCC-3′;
mouse iNOS forward primer, 5′- CAGCTGG GCTGTACAAACCTT-3′ and
reverse primer, 5′-CATTGGAAGTGAAGCGTT TCG-3′;
mouse IL-6 forward primer, 5′- CTTGGGACTGATGCTGGTGAC-3′ and
reverse primer, 5′- TTCTCATTTCCACGATTTCCCA-3′;
mouse IL-1β forward primer, 5′- TGTCTTGGCCGAGG ACTAAGG-3′ and
reverse primer, 5′-TGGGCTGGACTGTTTCTAATGC-3′;
and mouse IL-10 forward primer, 5′- GCTCT TACTGACTGGCATGAG-3′ and
reverse primer, 5′-CGCAGCTCTAGGAGCA TGTG-3′.