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Ariaiii

Manufactured by Tree Star
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The AriaIII is a high-performance flow cytometer designed for advanced cell analysis and sorting. It features precise fluidics, sensitive optics, and reliable electronics to enable accurate and efficient data acquisition. The core function of the AriaIII is to facilitate the identification, enumeration, and sorting of diverse cell populations within complex samples.

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Lab products found in correlation

F4 80 apc, by Thermo Fisher Scientific (2 mentions) Cd4 apc, by Thermo Fisher Scientific (2 mentions) Cd11b pe, by Thermo Fisher Scientific (2 mentions) Cd11b fitc, by Thermo Fisher Scientific (2 mentions) Ly6c fitc or apc cy7, by BD (2 mentions) Cd45r b220 pacific blue, by BD (2 mentions) Ccr2 alexafluor647, by BD (2 mentions) Cd4 l3t4 fitc, by Thermo Fisher Scientific (2 mentions) Ly6g pe cy7, by BD (2 mentions) Cd3e fitc, by BioLegend (2 mentions) Cd3e apc cy7, by BD (2 mentions) Cx3cr1 apc, by BD (2 mentions) Nk1 1 percp cy5, by Thermo Fisher Scientific (2 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Lsr fortessa, by Tree Star (1 mentions) Cba flex set, by BD (1 mentions) Live dead aqua 525, by Thermo Fisher Scientific (1 mentions)

3 protocols using ariaiii

1

Multiparameter Flow Cytometry Analysis

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Cell suspensions were treated with anti CD16/CD32 (2.4G2) and then surface stained with combinations of the following fluorochrome-conjugated antibodies: From Biolegend: CD3e–FITC (clone: 145 2C11), Ly6C–FITC or APC/Cy7 (clone: HK1.4), Ly6G–PE/Cy7 (clone: 1A8), CD3e–APC/Cy7 (clone: 145 2C11), CD45R/B220–Pacific Blue (clone: RA3 6B2), CCR2-AlexaFluor647 (clone: SA203G11), CX3CR1-APC (clone: SA011F11), IgG2A,κ-APC isotype control; from BD Biosciences: NK1.1–PerCP/Cy5.5 (clone: PK136); from Ebiosciences: F4/80–APC (clone: BM8), CD4 (L3T4)–FITC (clone: RM4 5), CD11b–FITC (clone: M1/70), CD11b–PE (clone: M1/70), CD4–APC (clone: GK1.5). Cells were acquired on FACSCanto or AriaIII flow cytometers using FACSDiVa software, and data were analyzed using FlowJo software (Tree Star).
Seregin S.S., Golovchenko N., Schaf B., Chen J., Eaton K.A, & Chen G.Y. (2016). NLRP6 function in inflammatory monocytes reduces susceptibility to chemically-induced intestinal injury. Mucosal immunology, 10(2), 434-445.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell suspensions were treated with anti CD16/CD32 (2.4G2) and then surface stained with combinations of the following fluorochrome-conjugated antibodies: From Biolegend: CD3e–FITC (clone: 145 2C11), Ly6C–FITC or APC/Cy7 (clone: HK1.4), Ly6G–PE/Cy7 (clone: 1A8), CD3e–APC/Cy7 (clone: 145 2C11), CD45R/B220–Pacific Blue (clone: RA3 6B2), CCR2-AlexaFluor647 (clone: SA203G11), CX3CR1-APC (clone: SA011F11), IgG2A,κ-APC isotype control; from BD Biosciences: NK1.1–PerCP/Cy5.5 (clone: PK136); from Ebiosciences: F4/80–APC (clone: BM8), CD4 (L3T4)–FITC (clone: RM4 5), CD11b–FITC (clone: M1/70), CD11b–PE (clone: M1/70), CD4–APC (clone: GK1.5). Cells were acquired on FACSCanto or AriaIII flow cytometers using FACSDiVa software, and data were analyzed using FlowJo software (Tree Star).
Seregin S.S., Golovchenko N., Schaf B., Chen J., Eaton K.A, & Chen G.Y. (2016). NLRP6 function in inflammatory monocytes reduces susceptibility to chemically-induced intestinal injury. Mucosal immunology, 10(2), 434-445.
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3

Cytokine Quantification in Lung Homogenates

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Cytokines present in lung homogenate supernatants were measured using a BD CBA flex set (BD Bioscience) as per the manufacturer's instructions [54] (link). Samples were analysed using a Becton Dickinson FACS Canto II flow cytometer. Data were analysed using FCAP Array software (Soft Flow Inc., Pecs, Hungary). Live/Dead-aqua 525 were purchased from Invitrogen. Briefly, cell suspensions were stained with Live/Dead Aqua viability dye at room temperature for 10 mins followed by staining with tetramer for 15 mins and cell surface marker antibodies for 30 mins. Cells were fixed with 1% paraformaldehyde before analysis by flow cytometry. All antibody and tetramer staining was performed at 4°C and in the dark. Samples were subsequently acquired on a Becton Dickinson LSR Fortessa or Aria III flow cytometer and data analyzed by Flowjo Software (Tree Star Inc, USA).
Jia X., Chua B.Y., Loh L., Koutsakos M., Kedzierski Ł., Olshansky M., Heath W.R., Xu J., Wang Z., & Kedzierska K. (2021). High expression of CD38 and MHC class II on CD8+T cells during severe influenza disease reflects bystander activation and trogocytosis.
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