The reactions were carried out in a thermostatted glass reactor (30 mL) agitated with an electronically controlled magnetic stirrer connected to a large reservoir (5000 mL) containing oxygen at 2 atm. The oxygen uptake was followed by a mass flow controller connected to a PC through an A/D board. The oxidation experiments were carried out in xylene (1.25 M substrate, substrate/Pd = 3000 (mol/mol), 80 °C, pO2 = 2 atm). The reaction was monitored by analyzing periodically withdrawn samples. Mass balances, in the analysis, were always 98% ± 3%. Analyses were performed using a HP 7820A gas chromatograph equipped with a capillary column HP-5 30 m × 0.32 mm, 0.25 μm Film, by Agilent Technologies(Milan, Italy). Authentic samples were analyzed to determine separation times. Quantitative analyses with external standard method (n-octanol) was used.
Hp 7820a gas chromatograph
Manufactured by Agilent Technologies
Sourced in Italy
The HP 7820A gas chromatograph is a laboratory instrument designed for the separation and analysis of chemical compounds. It utilizes a gas stream to carry sample molecules through a column where they are separated based on their respective physical and chemical properties. The separated components are then detected and measured, providing analytical data about the sample composition.
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Lab products found in correlation
2 protocols using hp 7820a gas chromatograph
1
Oxidation of Organic Substrates in Xylene
2
GC-FID and GC-MS Analysis of Essential Oil
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The essential oil was analyzed on a HP 7820A Gas Chromatograph (GC) equipped with a flame ionization detector (FID) using a capillary column (HP5 30 × 0.32 mm × 0.25 microns, Agilent). Column temperature: 50 °C (0 min) to 3 °C min -1 to 230 °C. Gun: 250°C Split (1:30). FID Detector: 250 °C. Carrier gas: hydrogen to 3 ml min -1 . Vol injection: 1 µl. Essential oil was diluted to 1% in chloroform. Data acquisition software used was Compact EZChrom Elite (Agilent). The quantitative analysis was performed using standard areas from the chromatograms obtained by GC-FID.
Subsequently, to quantify the different constituents, a CG-MS with Column: Rxi-1MS 923 30 m × 0.25 mm × 0.25 microns (Restek) was used. Column Temp: 50ºC (3 min), 3°C min -1 to 250°C. Injector: 250°C Split (1:10), GC-MS interface at 250 ºC. MS detector (electron impact at 70 eV) temperature was 250 ºC. Carrier gas: Helium up to 1.5 ml min -1 . Vol injection: 1 µl. Essential oil was diluted to 0.1% in chloroform. Data acquisition software used was GC-MS Solution (Shimadzu) with NIST11 library. Identification of peaks was made by comparison of the mass spectra obtained by GC-MS spectra with the NIST11 library and also by comparing the Kovats indices calculated by GC-FID and literature data.
Subsequently, to quantify the different constituents, a CG-MS with Column: Rxi-1MS 923 30 m × 0.25 mm × 0.25 microns (Restek) was used. Column Temp: 50ºC (3 min), 3°C min -1 to 250°C. Injector: 250°C Split (1:10), GC-MS interface at 250 ºC. MS detector (electron impact at 70 eV) temperature was 250 ºC. Carrier gas: Helium up to 1.5 ml min -1 . Vol injection: 1 µl. Essential oil was diluted to 0.1% in chloroform. Data acquisition software used was GC-MS Solution (Shimadzu) with NIST11 library. Identification of peaks was made by comparison of the mass spectra obtained by GC-MS spectra with the NIST11 library and also by comparing the Kovats indices calculated by GC-FID and literature data.
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