Isopropyl alcohol

Manufactured by Fujifilm

Sourced in Japan

Isopropyl alcohol is a clear, colorless, and flammable chemical solvent commonly used in laboratory settings. It serves as a cleaning agent, disinfectant, and drying agent for various laboratory equipment and surfaces.

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Lab products found in correlation

Chloroform, by Fujifilm (4 mentions) Ethanol, by Fujifilm (4 mentions) Fetal bovine serum (fbs), by Biosera (2 mentions) D glucose, by Fujifilm (2 mentions) Hygromycin b gold, by InvivoGen (2 mentions) Penicillin streptomycin glutamine mixed solution, by Nacalai Tesque (2 mentions) Nahco3, by Fujifilm (2 mentions) Hank s balanced salt solution, by Merck Group (2 mentions) Hematoxylin, by Fujifilm (2 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Kod plus neo, by Toyobo (1 mentions) Noti hf, by New England Biolabs (1 mentions) Kpni hf, by New England Biolabs (1 mentions) Lentiviral high titer packaging mix, by Takara Bio (1 mentions) Antibiotic antimycotic mixed stock solution, by Nacalai Tesque (1 mentions) Plvsin cmv neo plasmid, by Takara Bio (1 mentions) Ecori hf, by New England Biolabs (1 mentions) Dulbecco s modified eagle s medium dmem, by Nacalai Tesque (1 mentions) Sodium dodecyl sulfate (sds), by Fujifilm (1 mentions)

Spelling variants of this product

Isopropyl alcohol (IPA) (2 citations)

13 protocols using isopropyl alcohol

Aerogel Particle Coating Preparation

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Example 1

10 g of the aerogel particle prepared as described above, 5 g of aramid fiber (Technora, manufactured by Teijin Limited, average fiber length: 6 mm), 100 g of isopropyl alcohol (reagent, manufactured by Wako Pure Chemical Industries Ltd.), and 2 g of carboxymethyl cellulose ammonium (reagent, manufactured by Wako Pure Chemical Industries Ltd.) were placed in a 300 mL separable flask, followed by stirring with a mechanical stirrer at 150 rpm for 15 minutes, and thus an isopropyl alcohol dispersion was obtained. Subsequently, 100 g of water was added to the dispersion to dissolve carboxymethyl cellulose ammonium therein to obtain a coating liquid 1.

US11787957B2. Coating solution, method for producing coating film, and coating film (2023-10-17). HITACHI CHEMICAL COMPANY, LTD. [JP]. Inventors: Hiroyuki Izumi [JP], Tatsuya Makino [JP], Tomohiko Kotake [JP], Satoshi Takayasu [JP], Kaito Kogure [JP].

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RNA Isolation and Gene Expression Analysis of Biliary Cysts

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Cyst samples were dispersed in 500 μl Trizol reagent (Invitrogen) by pipetting. Chloroform (Wako), as much as 100 μl was added into the samples, mixed, incubated at room temperature for 5 min, and centrifuged at 12000 g for 15 min. The transparent aqueous phase was separated, mixed with 250 μl isopropyl alcohol (Wako), incubated for 15 min at RT, and centrifuged at 12000 g for 15 min. Pellet on the bottom of the microtube was washed by 1 ml EtOH, mixed, and centrifuged at 7500 g for 5 min. After removed the EtOH, 10 μl RNAase free water was added to the pellet and mixed. RNA samples with concentration at least 100 μg/ml were used to obtain cDNA. An RT reaction was obtained using the Tra Ace qPCR RT Master Mix with gDNA Remover (Toyobo, FSQ-301) protocols employing these phases: 37 °C, 15 min; 50 °C, 5 min; 98 °C, 5 min; and 4 °C, hold. Subsequently, we qualitatively assessed the expression of biliary cell-related function by qRT-PCR using several specific biliary markers followed by cDNA amplification (Thunderbird Sybr qPCR mix) using a real-time PCR (StepOnePlus) system. We analysed six markers (Eurofins) (Table 2) to assess gene expression of the cysts with β-actin serving as a housekeeping gene and employing this cycling condition: pre-denaturation at 95 °C for 60 sec, denaturation at 95 °C for 15 sec, and extension (40 cycles) at 60 °C for 60 sec.

Rizki-Safitri A., Shinohara M., Miura Y., Danoy M., Tanaka M., Miyajima A, & Sakai Y. (2018). Efficient functional cyst formation of biliary epithelial cells using microwells for potential bile duct organisation in vitro. Scientific Reports, 8, 11086.

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Total RNA Extraction and cDNA Synthesis

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Total RNA was extracted from cultured PMNs and PBMCs by adding 0.2 ml of chloroform to the TRlzol-containing tubes, vortexing
them vigorously for 15 sec, and incubating them at room temperature for 3 min. Samples were centrifuged at 18,300 ×
g for 15 min at 4 C. The upper aqueous phase was transferred into a 1.5-ml tube, and 500 µl isopropyl
alcohol (Wako) was added and homogenized by inverting the tube. After incubation at room temperature for 10 min, the samples
were centrifuged at 18,300 × g for 10 min at 4 C. The supernatant was carefully recovered, and 900 µl 80%
ethanol (Wako) was added, followed by centrifugation at 11,700 × g for 5 min at 4 C. After removing the
supernatant, the RNA pellet was air-dried for 30 min, and sterilized water was then added. The extracted total RNA was stored
at –80 C until use for cDNA synthesis. cDNA was generated by reverse transcription (RT) using a PrimeScript RT reagent kit
with gDNA Eraser (Perfect Real Time; Takara Bio) according to the manufacturer’s method. The synthesized cDNA was stored at
–30 C.

KAWASAKI Y., AOKI Y., MAGATA F., MIYAMOTO A., KAWASHIMA C., HOJO T., OKUDA K., SHIRASUNA K, & SHIMIZU T. (2014). The Effect of Single Nucleotide Polymorphisms in the Tumor Necrosis Factor-α Gene on Reproductive Performance and Immune Function in Dairy Cattle. The Journal of Reproduction and Development, 60(3), 173-178.

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Lentiviral Vector Construction and Transduction

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The pSelect-zeo-HSV1tk plasmid, G418, Hygromycin B Gold, LB Broth Base, and LB Agar were purchased from InvivoGen Co. (San Diego, CA, USA). Lipofectamine 3000 was purchased from Invitrogen Co. (Carlsbad, CA, USA). pLVSIN-CMV-Neo plasmid, and Lentiviral High Titer Packaging Mix were purchased from Takara Bio Inc. (Shiga, Japan). KOD-Plus-Neo was purchased from TOYOBO Co., Ltd. (Osaka, Japan). Competent NEB 10-beta E. coli (High efficiency), SOC medium, KpnI-HF, EcoRI-HF, and NotI-HF were purchased from New England Biolabs Inc. (Ipswich, MA, USA). Ganciclovir hydrate was purchased from Tokyo Kasei Kogyo Co., Ltd. (Tokyo, Japan). Hank’s Balanced Salt solution was purchased from Sigma-Aldrich (St. Louis, MO, USA). Chloroform, isopropyl alcohol, ethanol, NaHCO₃, D-(+)-glucose, 0.4 w/v% trypan blue solution, and pEBMulti-Hyg plasmid were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Fetal bovine serum was purchased from Biosera (East Sussex, UK) or Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s Modified Eagle’s medium (DMEM), antibiotic-antimycotic mixed stock solution, and penicillin-streptomycin-glutamine mixed solution were purchased from Nacalai Tesque, Inc. (Kyoto, Japan) or Nissui Seiyaku (Tokyo, Japan). All the other chemicals used were of the highest grade available commercially.

Tsujimura M., Kusamori K., Katsumi H., Sakane T., Yamamoto A, & Nishikawa M. (2019). Cell-based interferon gene therapy using proliferation-controllable, interferon-releasing mesenchymal stem cells. Scientific Reports, 9, 18869.

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Isolation and Culture of Primary Cells

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Hygromycin B Gold was purchased from InvivoGen Co. (San Diego, CA, USA). HEPES was purchased from Dojindo Laboratories (Kumamoto, Japan). Hank's balanced salt solution was purchased from Sigma-Aldrich (St. Louis, MO, USA). Deoxyribonuclease I from bovine pancreas, precrystalline, DISPASE II, NP-40 substrate, tris (hydroxymethyl) aminomethane, sodium deoxycholate, sodium dodecyl sulfate, chloroform, isopropyl alcohol, ethanol, NaHCO₃, d-(+)-glucose, and 0.4 w/v % trypan blue solution were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Collagenase D was purchased from Roche Diagnostics, Inc. (Mannheim, Germany). Fetal bovine serum (FBS) was purchased from Biosera (East Sussex, UK). Dulbecco's modified Eagle's medium (DMEM) was purchased from Nissui Pharmaceutical Co. Ltd. (Tokyo, Japan). Mitomycin C, a penicillin-streptomycin-glutamine mixed solution, and 100 mM sodium pyruvate solution (100 × ) were purchased from Nacalai Tesque Inc. (Kyoto, Japan). All the other chemicals used were of the highest commercially available grade.

Tsujimura M., Kusamori K., Takamura K., Ito T., Kaya T., Shimizu K., Konishi S, & Nishikawa M. (2022). Quality evaluation of cell spheroids for transplantation by monitoring oxygen consumption using an on-chip electrochemical device. Biotechnology Reports, 36, e00766.

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Oil Red O Staining of Adipocytes

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The cells cultured in chambers were fixed in 4% cold PFA for 10 min and rinsed with 60% isopropyl alcohol (Wako Pure Chemical Industries, Ltd.). Oil Red O stain (200 mg; Sigma-Aldrich) was dissolved in 10 ml 60% isopropyl alcohol and filtered. Fixed cells were stained with 2% Oil Red O solution for 5 min at room temperature. Slides were then rinsed with deionized water, counterstained with hematoxylin (Wako Pure Chemical Industries, Ltd.) for 15 min and observed under a microscope.

Higa K., Satake Y, & Shimazaki J. (2017). The characterization of human oral mucosal fibroblasts and their use as feeder cells in cultivated epithelial sheets. Future Science OA, 3(4), FSO243.

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Microfluidic Chip Fabrication via Soft Lithography

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Detailed procedures for the fabrication of a microfluidic chip are as described in Yasaki
et al.^{19 (link)} In summary, a soft lithography technique was used for silicone elastomer
polydimethylsiloxane (PDMS) molding. The mold fabrication process for PDMS microstructures
was performed according to the SU-8 Data Sheet (Nippon Kayaku, Tokyo, Japan). SU-8 (3025,
Nippon Kayaku) was coated on the silicon substrate (3 in., Ferrotec, Tokyo, Japan) by
using a spin coater (IF-D7, Mikasa, Tokyo, Japan). After soft baking, this layer was
exposed to ultraviolet light through a photomask in order to form patterns by using a mask
aligner (M-1S, Mikasa, Tokyo, Japan). After the development, the substrate was washed by
rinsing with SU-8 Developer (Nippon Kayaku, Tokyo, Japan) and isopropyl alcohol (Wako Pure
Chemical Industries, Tokyo, Japan). A PDMS prepolymer solution containing a mixture of
10:1 mass ratio of PDMS oligomers and a reticular agent from a Sylgard 184 Kit (SILPOT 184
Dow Corning, Toray, Tokyo, Japan) was poured onto the silicon substrate and cured for 2 h
at 80 °C. The patterned PDMS plate was cut using a surgical scalpel blade (X-ACTO, Elmer’s
Products, Westerville, OH, USA).

Onoshima D., Hattori Y., Yukawa H., Ishikawa K., Hori M, & Baba Y. (2018). Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion. Cell Medicine, 10, 2155179017733152.

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Oil Red O Staining for Lipid Quantification

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Cells cultured in chambers were fixed in 4% cold paraformaldehyde for 10 min and rinsed with 60% isopropyl alcohol (Wako, Osaka, Japan). An amount of 200 mg of Oil Red O (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 10 mL 60% isopropyl alcohol and filtered. Fixed cells were stained with a 2% Oil Red O solution for 5 min at RT, after which they were rinsed with deionized water, counterstained with hematoxylin (Wako, Osaka, Japan) for 15 min, and observed using a microscope.

Yamane S., Higa K., Umezawa T., Serikawa M., Shimazaki J, & Abe S. (2016). Engineered three-dimensional rabbit oral epithelial–mesenchymal–muscular hybrid sheets. International Journal of Oral Science, 8(3), 145-154.

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Negative Photoresist and Aluminum Etching

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Negative photoresist was purchased from MicroChem (KMPR-1000 series; product number: KMPR 1035; Newton, MA, USA). Acetone (99.5%) and isopropyl alcohol (99.7%) were purchased from Wako Chemicals (Tokyo, Japan). Aluminum etchant was obtained by mixing H₂PO₄, HNO₃, CH₃COOH, and deionized water. H₂PO₄, HNO₃, and CH₃COOH were purchased from Wako Chemicals. Developer and positive photoresist were purchased from TOKYO OHKA KOGYO (product numbers: NMD-3 and OFPR-800LB 100cP; Kanagawa Prefecture, Japan). Cover glasses were purchased from Matsunami Glass (product number: Micro Cover Glass No. 4; dimensions: 0.35–0.45 mm in thickness, 40×30 mm; Chiba Prefecture, Japan).

Takahashi H., Jung Heo Y., Arakawa N., Kan T., Matsumoto K., Kawano R, & Shimoyama I. (2016). Scalable fabrication of microneedle arrays via spatially controlled UV exposure. Microsystems & Nanoengineering, 2, 16049.

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HPLC-based Glycosphingolipid Quantification

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HPLC-grade methanol (MeOH), distilled water (H₂O), acetonitrile (ACN), isopropyl alcohol (IPA), formic acid (FA), analytical-grade chloroform (CHCl₃), hexane, and other reagents were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Iatrobeads were obtained from Iatron Laboratories (Tokyo, Japan). Standard Gb3 (porcine RBC) was purchased from Matreya LLC (State College, PA, USA). Standard lyso-Gb3 and a glycine derivative of lyso-Gb3 (Gly-lyso-Gb3) were prepared as previously described [23 (link)].

Taguchi A., Ishii S., Mikame M, & Maruyama H. (2023). Distinctive accumulation of globotriaosylceramide and globotriaosylsphingosine in a mouse model of classic Fabry disease. Molecular Genetics and Metabolism Reports, 34, 100952.

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