Serum creatinine, urea, fructosamine and urinary creatinine were analyzed on an ARCHITECT c16000 Clinical Chemistry Analyzer (Abbott Diagnostics, Lake Forest, IL, USA). Glycemia was measured using an AlphaTRAK blood glucose monitoring system (Abbott Laboratories, North Chicago, IL, USA). Serum insulin was assayed by a Rat ultrasensitive ELISA kit (ALPCO, Salem, NH, USA). The presence of glycosuria was defined by a positive Diastix™ (Bayer Corp. Diagnostics, Tarrytown, NY, USA) test (>14mmol/L) on two consecutive mornings.
Rat ultrasensitive elisa kit
Manufactured by ALPCO
Sourced in United States
The Rat Ultrasensitive ELISA Kit is a laboratory tool designed for the quantitative measurement of target analytes in rat biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide sensitive and accurate results.
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Lab products found in correlation
2 protocols using rat ultrasensitive elisa kit
1
Serum and Urinary Biomarkers in Metabolic Assessment
2
Insulin Secretion in INS-1 Cells
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INS-1 cells were washed and incubated in KRB buffer. Cells were pre-incubated in KRB buffer (0.1 mM glucose) with AD extracts for 2 h and then incubated in KRB buffer supplemented with 3 or 17.5 mM glucose for an additional 90 min at 37°C, 5% CO2. The insulin levels were measured using a rat ultrasensitive ELISA kit (Alpco Diagnostics, NH, USA), according to manufacturer’s instruction. The insulin content was normalized to the protein content of the corresponding cell lysates.
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