04 001 1acs

Manufactured by Sartorius

Sourced in Israel, United States, China

The 04-001-1ACS is a laboratory equipment product from Sartorius. It is designed for general laboratory use. The core function of this product is to provide a versatile and reliable solution for various laboratory applications. Further details on the intended use or specific features of this product are not available.

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Lab products found in correlation

Fetal bovine serum (fbs), by Sartorius (4 mentions) Lipo2000, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Beyotime (2 mentions) 01 100 1acs, by Sartorius (2 mentions) Dulbecco s modified eagle s medium f12, by Merck Group (2 mentions) Interferon γ ifn γ, by Thermo Fisher Scientific (2 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Merck Group (2 mentions) Bl306a, by Biosharp (2 mentions) Egf 50482 mnch lc13ja1403, by Sino Biological (2 mentions) Bfgf 50177 m08h lc12se1702, by Sino Biological (2 mentions) Pancreatin from porcine pancreas, by Merck Group (1 mentions) 10 013 cvr, by Corning (1 mentions) Collagenase 2, by Thermo Fisher Scientific (1 mentions) Percoll, by GE Healthcare (1 mentions) C8052, by Merck Group (1 mentions) C0222, by Beyotime (1 mentions) Egf phg0311, by Thermo Fisher Scientific (1 mentions) Cholera toxin, by Merck Group (1 mentions)

28 protocols using 04 001 1acs

Targeting STAT5 and IL-6 in Cancer Cells

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The human breast carcinoma cells MDA-MB-231, T47D and human umbilical venin endothelial cells (HUVEC) were obtained from Core Technology Facility of Center for Excellence in Molecular Cell Science, CAS. The MDA-MB-231 and T47D cells were cultured in high glucose Dulbecco's modified Eagle's medium (06-1055-57-1ACS, DMEM, Biological Industries, Israel) containing 10% fetal bovine serum (04-001-1ACS, Biological Industries, Israel) and 100U/ml penicillin/streptomycin (SV30010, Hyclone, Logan, UT). The HUVEC were cultured in RPMI 1640 medium (01-100-1ACS, Biological Industries, Israel) containing 10% fetal bovine serum (04-001-1ACS, Biological Industries, Israel) and 100 U/ml penicillin/streptomycin (SV30010, Hyclone, Logan, UT). Cells were cultured at 37°C in a humidified atmosphere with 5% CO₂.
The MDA-MB-231 and T47D cells were treated either with 10 μM PDTC (S3633, Selleck), 8nM AZD3965 (S7339, Selleck), or with 5 μM 5-MPN (S656801, Sigma-Aldrich) 24hrs for Western blot.
The HUVEC in 6-well plates were either transfected with a siRNA targeting STAT5A (s13535, Ambion), STAT5B (s13539, Ambion) or non-silencing control using Dharmafect1 (T-2001-03, Dharmacon) transfection reagent according to the manufacturer's protocol, or treated with anti-IL-6 (1:1000, WL02841, Wanleibio) 24hrs for Western blot.

Li D., Tang J., Gao R., Lan J., Shen W., Liu Y., Chen Y., Sun H., Yan J., Nie Y, & Luo N. (2022). PFKFB4 promotes angiogenesis via IL-6/STAT5A/P-STAT5 signaling in breast cancer. Journal of Cancer, 13(1), 212-224.

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Isolation and Culture of Neonatal Rat Cardiomyocytes

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Hearts from neonatal Sprague-Dawley rats (Beijing Vital River Laboratory Animal Technology Co. Ltd., Beijing, China) aged 1−3 days old were harvested, minced, and digested with Collagenase II (17101015; Gibco, Waltham, MA, USA) and Pancreatin from porcine pancreas (P3292; Sigma-Aldrich). Using differential velocity adherent technique, Percoll (17-0891-01; GE healthcare, Chicago, IL, USA) treatment, and centrifugation, NRCMs were isolated and purified as previously reported.³⁰ (link) NRCMs were maintained in Dulbecco's modified Eagle's medium (DMEM; 10-013-CVR; Corning, Corning, NY, USA) containing 4.5 g/L glucose supplemented with 10% horse serum (16050-122; Gibco) and 5% fetal bovine serum (04-001-1ACS; BioInd, Kibbutz Beit Haemek, Israel) before further treatment.

Bei Y., Huang Z., Feng X., Li L., Wei M., Zhu Y., Liu S., Chen C., Yin M., Jiang H, & Xiao J. (2022). Lymphangiogenesis contributes to exercise-induced physiological cardiac growth. Journal of Sport and Health Science, 11(4), 466-478.

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Drosophila S2 Cells Knockdown Protocol

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Drosophila Schneider 2 (S2) cells were grown at 28 °C in Schneider’s medium (21720024, Gibco, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (04-001-1ACS, Bioind, Israel)^{36 (link)}. For knockdown of Prp3, S2 cells were transfected using Lipofectamine 2000 (Lipo2000; 11668019, Invitrogen, USA). siRNAs were designed and synthesized by GenePharma company (Suzhou, China). Detailed information is as follows: negative control, F: 5-UUCUCCGAACGUGUCACGUTT-3, R: 5-ACGUGACACGUUCGGAGAATT-3; Prp3 siRNA-1660, F: 5-GCUCAUGCUAACGCGCAUUTT-3, R: 5-AAUGCGCGUUAGCAUGAGCTT-3; Prp3 siRNA-354, F: 5-GCAAGCGGGCCCUGAGUAATT-3, R: 5-UUACUCAGGGCCCGCUUGCTT-3.

Chen X., Luan X., Zheng Q., Qiao C., Chen W., Wang M., Yan Y., Xie B., Shen C., He Z., Zhang J., Liu M., Hu X., Li H., Zheng B., Fang J, & Yu J. (2019). Precursor RNA processing 3 is required for male fertility, and germline stem cell self-renewal and differentiation via regulating spliceosome function in Drosophila testes. Scientific Reports, 9, 9988.

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Culturing Breast Cancer Cell Lines

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The breast cancer cell line MCF7 was cultured in DMEM/high glucose (SH30243.01B, HyClone, Logan, UT) with 10% fetal bovine serum (FBS) (04-001-1ACS, Biological Industries, Kibbutz Beth Haemek, Israel) and 1% penicillin/streptomycin (p/s) (C0222, Beyotime Institute of Biotechnology, Jiangsu, China). SKBR3, HCC1806 and 786-0 cells were cultured in RPMI-1640 (SH30809.01B, HyClone) containing 10% FBS and 1% p/s. MDA-MB-231 cells were cultured in DMEM/F12 (SH30023.01B, HyClone), supplemented with 10% FBS and 1% p/s. The immortalized human breast epithelial cell line MCF10A was maintained in DMEM/F12, supplemented with 5% horse serum (16050-130, Gibco, New Zealand), 20 ng/mL EGF (PHG0311, Invitrogen, Carlsbad, CA), 0.5 mg/mL hydrocortisone (MB1567, Meilunbio, Dalian, China), 100 ng/mL cholera toxin (C8052, Sigma-Aldrich, St. Louis, MO), 10 μg/mL insulin (Wanbang Biopharmaceuticals, Xuzhou, China), and 1% p/s. The cells were purchased from Conservation Genetics CAS Kunming Cell Bank. Cell lines were tested to be mycoplasma-free by PCR (16 (link)).

Yu Q., Pu S.Y., Wu H., Chen X.Q., Jiang J.J., Gu K.S., He Y.H, & Kong Q.P. (2019). TICRR Contributes to Tumorigenesis Through Accelerating DNA Replication in Cancers. Frontiers in Oncology, 9, 516.

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Cell Culture and Starvation Induction Protocol

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The cells of each cell line were derived from ATCC and stored in liquid nitrogen. After being passed 3 times, the cell lines were started to be used for experiments and the passage number was no more than 5. The mixed medium cell culture components were as follows: DMEM/F12 medium (Gibco, c11330500bt, Carlsbad, CA, USA), 10% fetal bovine serum (FBS, 04-001-1ACS, Biological Industries, Israel), and 1% antibiotic mixture (penicillin/streptomycin, Beyotime, c0222, Shanghai, China). RWPE‐1 cells were grown in keratinocyte serum-free medium (K‐SFM, Gibco, 17005042, Carlsbad, CA, USA) supplemented with 10% FBS and 1% antibiotic mixture. Cells were cultured in a humidified incubator (Esco, CLM-170B-8-NF, Singapore) containing 5% CO₂ at 37°C. The starvation induction condition is serum withdrawal, and the remaining conditions are unchanged. The following drugs were used: carbachol (MedChemExpress, HY-B1208, Monmouth Junction, NJ, USA, dissolved in ddH₂O), pirenzepine (HY-17037, dissolved in ddH₂O), proteinase inhibitor E64 (HY-15282, dissolved in DMSO), pepstatin A (HY–P0018, dissolved in DMSO), bafilomycin A1 (HY-100558, dissolved in DMSO), and 3-methyladenine (HY-19312, dissolved in DMSO).

Wang Q., Chen J., Zhang M., Wang H., Zeng Y., Huang Y, & Xu C. (2022). Autophagy Induced by Muscarinic Acetylcholine Receptor 1 Mediates Migration and Invasion Targeting Atg5 via AMPK/mTOR Pathway in Prostate Cancer. Journal of Oncology, 2022, 6523195.

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A549 Cell Culture Protocol

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A549 cells were obtained from the China Center for Type Culture Collection (Wuhan, China). Cells were cultured in high glucose DMEM containing 10% fetal bovine serum (#04-001-1ACS, Biological Industries, Kibbutz Beit Haemek, Israel), 100 IU/ml penicillin G, and 100 μg/ml streptomycin (#SV30010, HyClone, Logan, Utah, USA) at 37°C in a 5% CO₂ atmosphere. The medium was changed every two days. Cells were digested when confluency reached 80~90%. Cell monolayers were harvested in 0.25% trypsin-EDTA solution (#25200-056, Invitrogen, Grand Island, NY, USA).

Ma W., Wang Z., Zhao Y., Wang Q., Zhang Y., Lei P., Lu W., Yan S., Zhou J., Li X., Yu W., Zhong Y., Chen L, & Zheng T. (2021). Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation. Oxidative Medicine and Cellular Longevity, 2021, 6614574.

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Culture conditions for NK and myeloma cells

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The NK cell line KHYG-1 was cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) (#04-001-1ACS, Biological Industries, Kibbutz Beit-Haemek, Israel), 10 ng/mL human IL-2 (#200-02, PeproTech, Rocky Hill, New Jersey, USA), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. The NK cell line NK92 was cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, 10% horse serum, 10 ng/mL IL-2, 2 mM glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. The human multiple myeloma cell line MM.1S and leukemia cell line K562 were grown in RPMI-1640 supplemented with 10% FBS, 2 mM glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. All cells were maintained at 37°C in a humidified atmosphere containing 5% CO₂. Normoxic or hypoxic cell culture conditions were obtained by culturing cells in a sealed incubator flushed with a mixture of 20% O₂, 5% CO₂, and 75% N₂ or the mixture of 1% O₂, 5% CO₂, and 94% N₂, respectively.

Teng R., Wang Y., Lv N., Zhang D., Williamson R.A., Lei L., Chen P., Lei L., Wang B., Fu J., Liu X., He A., O'Dwyer M, & Hu J. (2020). Hypoxia Impairs NK Cell Cytotoxicity through SHP-1-Mediated Attenuation of STAT3 and ERK Signaling Pathways. Journal of Immunology Research, 2020, 4598476.

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Inflammatory HT29 Epithelial Cell Model

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HT29 human intestinal epithelial cells were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in DMEM (Gibco) with 10% foetal bovine serum (04-001-1ACS, Biological Industries, Kibbutz Beit-Haemek, Israel) at 37 °C in the presence of 5% CO₂. TNFα (10 ng/mL, Z01001, GenScript Biotech, Nanjing, China) was used in cell culture with HT29 cells for 12 h to mimic an inflammatory background [30 (link)].

Zhang J., Wang W., Zhu S, & Chen Y. (2021). Increased SERPINA3 Level Is Associated with Ulcerative Colitis. Diagnostics, 11(12), 2371.

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Cell Line Cultivation and Maintenance

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PWR-1E cell line was purchased from the American Type Culture Collection (ATCC, USA). The cells were cultured in complete keratinocyte serum-free medium (Invitrogen, USA) was used. The BPH-1 cell line was purchased from Keygen Biotech (KG1008, China). The cells were maintained in RPMI-1640 medium (01-100-1ACS, Biological Industries, Israel) containing 1% streptomycin and 1% penicillin and supplemented with 10% fetal bovine serum (04-001-1ACS, Biological Industries, Israel). The two cell lines were cultured in a humidified incubator at 37°C with 5% carbon dioxide.

Song H., Shen Q., Hu S, & Jin J. (2020). The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling. Biology Open, 9(11), bio053447.

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Culturing Human Melanoma Cell Lines

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The human melanoma cell lines ME4405, A375, SK-Mel-5 and Sk-Mel-28 (maintained in our laboratory) and the human melanocyte cell line PIG1 (a gift from the Department of Dermatology, Xiangya Third Hospital) were used in this study. Melanoma cells were grown in DMEM (01-052-1A, Biological Industries, Beit HaEmek, Israel) or Opti-MEM for PIG1 (31985070, Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (04-001-1ACS, Biological Industries) and 1% penicillin–streptomycin (03-031-1B, Biological Industries) at 37 °C and 5% CO₂.

Guo Y., Zhang X., Wang L., Li M., Shen M., Zhou Z., Zhu S., Li K., Fang Z., Yan B., Zhao S., Su J., Chen X, & Peng C. (2021). The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma. Cancer Cell International, 21, 487.

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