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Coulter xl apparatus

Manufactured by Beckman Coulter
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The Coulter XL apparatus is a compact, automated hematology analyzer designed for clinical laboratories. It utilizes the Coulter principle to accurately measure and analyze various blood cell parameters, including red blood cells, white blood cells, and platelets. The Coulter XL provides reliable and consistent results to support clinical decision-making.

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Lab products found in correlation

Anti b220 apc, by BioLegend (2 mentions) Anti igg2a pe, by BioLegend (2 mentions) Anti igg3 fitc, by BD (2 mentions) Anti igg2b pe, by BioLegend (2 mentions) Anti igg1 fitc, by BioLegend (2 mentions) Anti iga fitc, by BD (1 mentions)

3 protocols using coulter xl apparatus

1

Multiparameter Flow Cytometry Analysis

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Cell suspensions from spleen, liver and LNs were stained with various antibodies (Southern Biotechnologies or eBiosciences): SpectralRed (PC5)-conjugated anti-B220, phycoerythrin-labeled (PE) anti-CD19, anti-CD138, FITC-conjugated anti-human IgA and PE anti-mouse CD43. Cells were analyzed on a Coulter XL apparatus (Beckman Coulter, Fullerton, CA).
Amin R., Marfak A., Pangault C., Oblet C., Chanut A., Tarte K., Denizot Y, & Cogné M. (2014). The class-specific BCR tonic signal modulates lymphomagenesis in a c-myc deregulation transgenic model. Oncotarget, 5(19), 8995-9006.
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2

Activated B cell phenotyping

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At day 4 post-stimulation, B cells were washed and stained with anti-B220 APC (BioLegend) and either anti-IgG3-FITC (BD-Pharmingen), anti-IgG2b-PE (BioLegend), anti-IgG2a-PE (BioLegend) or anti-IgG1-FITC (BioLegend). Activated B cells from AID-deficient mice were included as negative controls. Data were obtained on 3 × 104 viable cells by using a Coulter XL apparatus (Beckman Coulter).
Santos J.M., Braikia F.Z., Oudinet C., Haddad D., Conte C., Dauba A, & Khamlichi A.A. (2018). Duplication of a germline promoter downstream of the IgH 3′ regulatory region impairs class switch recombination. Scientific Reports, 8, 9164.
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3

B Cell Isotype Analysis

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At day 4 post-stimulation, cells were washed and stained with anti-B220 APC (BioLegend) and either anti-IgG3-FITC (BD-Pharmingen), anti-IgG1-FITC (BioLegend), anti-IgG2b-PE (BioLegend), anti-IgG2a-PE (BioLegend) or anti-IgA-FITC (BD-Pharmingen). Data were obtained on 3 × 104 viable cells by using a Coulter XL apparatus (Beckman Coulter).
Santos J.M., Oudinet C., Schöne L., Dauba A, & Khamlichi A.A. (2019). Essential role of the initial activation signal in isotype selection upon deletion of a transcriptionally committed promoter. Scientific Reports, 9, 18543.
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