Fasting glucose, total, and high-density lipoprotein (HDL) cholesterol levels were measured in plasma using Infinity™ Glucose Hexokinase Liquid Stable Reagent, Infinity™ Cholesterol, and HDL-CHOLESTEROL Plus Direct method, respectively (Thermo Fisher Scientific Inc., Waltham, MA, USA). Insulin levels were quantified by enzyme immunoassay kits (Mercodia AB, Uppsala, Sweden). Lipopolysaccharide binding protein (LBP) was measured by mouse LBP ELSA kit (Hycult Biotech Inc., Wayne, NJ, USA). Serum amyloid A (SAA) levels were determined by “PHASE” ™ Murine Serum Amyloid A Assay (Tridelta Development Ltd., Maynooth, Ireland). Cytokine levels were measured in plasma (dilution 1:1) using Meso Scale Discovery V-PLEX proinflammatory panel one kit (#K15048D-1) as described in section 2.8. In this case, the calculated detection limits were the following: IFN-γ (0.00988–909 pg/mL), IL-1β (0.374–1,765 pg/mL), IL-2 (0.194–2,595 pg/mL), IL-4 (0.0266–1,680 pg/mL), IL-5 (0.224–1,027 pg/mL), IL-6 (0.335–5,440 pg/mL), IL-10 (0.103–3,930 pg/mL), IL-12p70 (1.79–31,400 pg/mL), KC/GRO (0.0968–1,960 pg/mL), TNF-α (0.0256–619 pg/mL).
Enzyme immunoassay kits
Manufactured by Mercodia
Sourced in Sweden
Enzyme immunoassay kits are laboratory tools used to measure the concentration of specific analytes, such as proteins or hormones, in a sample. These kits employ the principles of immunoassay, where an antibody-antigen interaction is detected and quantified using an enzyme-based detection system.
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Lab products found in correlation
2 protocols using enzyme immunoassay kits
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Metabolic and Inflammatory Biomarkers
2
Metabolic and Inflammatory Biomarkers
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Fasting glucose, total, and high-density lipoprotein (HDL) cholesterol levels were measured in plasma using Infinity™ Glucose Hexokinase Liquid Stable Reagent, Infinity™ Cholesterol, and HDL-CHOLESTEROL Plus Direct method, respectively (Thermo Fisher Scientific Inc., Waltham, MA, USA). Insulin levels were quantified by enzyme immunoassay kits (Mercodia AB, Uppsala, Sweden). Lipopolysaccharide binding protein (LBP) was measured by mouse LBP ELSA kit (Hycult Biotech Inc., Wayne, NJ, USA). Serum amyloid A (SAA) levels were determined by “PHASE” ™ Murine Serum Amyloid A Assay (Tridelta Development Ltd., Maynooth, Ireland). Cytokine levels were measured in plasma (dilution 1:1) using Meso Scale Discovery V-PLEX proinflammatory panel one kit (#K15048D-1) as described in section 2.8. In this case, the calculated detection limits were the following: IFN-γ (0.00988–909 pg/mL), IL-1β (0.374–1,765 pg/mL), IL-2 (0.194–2,595 pg/mL), IL-4 (0.0266–1,680 pg/mL), IL-5 (0.224–1,027 pg/mL), IL-6 (0.335–5,440 pg/mL), IL-10 (0.103–3,930 pg/mL), IL-12p70 (1.79–31,400 pg/mL), KC/GRO (0.0968–1,960 pg/mL), TNF-α (0.0256–619 pg/mL).
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