Histochemical staining for the activity of mitochondrial succinate dehydrogenase (complex II) and cytochrome c oxidase (complex IV) in ovaries was performed as previously described, with modifications (Wang et al., 2019 (link)). Ovaries were dissected in PBS and incubated in cytochrome c oxidase assay medium (100 µM cytochrome c, 4 mM diaminobenzidine tetrahydrochloride, and 20 µg/ml catalase in 0.2 M phosphate buffer, pH 7.0) at room temperature for 30 min. The ovaries were then washed in PBS three times and incubated in succinate dehydrogenase assay medium (130 mM sodium succinate, 200 µM phenazine methosulphate, 1 mM sodium azide, and 1.5 mM nitroblue tetrazolium in 0.2 M phosphate buffer, pH 7.0) at room temperature for 10 min. After washing in PBS three times, ovaries were dehydrated in a graded ethanol series (70%, 95%, 2 × 100%), cleared in Histoclear (National Diagnostics) for 15 min, and mounted in dibutyl phthalate in xylene (VWR) overnight. Bright-field images were collected on a Zeiss Axio Observer Z1 microscope (Objective C-Apochromat 40×/1.1 W Corr, Leica LAS EZ software, Leica EC4 Digital Camera, water as immersion medium).
Ec4 digital camera
Manufactured by Leica
The EC4 Digital Camera is a high-quality imaging device designed for laboratory and scientific applications. It features a robust, compact design and delivers reliable, accurate digital imaging capabilities.
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Lab products found in correlation
3 protocols using ec4 digital camera
1
Histochemical Assay of Mitochondrial Enzymes in Ovaries
2
Pest Damage Symptoms in Infested Pods
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All the visible damage symptoms of the pest were recorded by observing the infested pods (n = 25) directly in the field. However, all the concealed damage symptoms (appearance of galleries inside pod husk, mucilage, and beans, and the location of immature stages of the pest within the infested pods) were systematically recorded after cutting open the infested pods (n = 20) in the laboratory. The length and width of the insect galleries (n = 20) from infested pods were measured using a stereomicroscope (Leica M10 equipped with an EC4 Digital camera).
3
Insect Colony Observation and Identification
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Insect colonies on the collected leaf samples were observed under a microscope for their egglaying pattern, life stages and colony structure. A Leica stereomicroscope M10, equipped with an EC4 Digital Camera was used for imaging pupal and adult taxonomic features and subsequent identification of various species. As puparia and male genitalia play an important role in the identification of P. bondari and P. minei, permanent slide mounts of the pupae and the male genitalia were prepared and observed under a compound microscope (Leica DMLB2) (Martin, 2004) .
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