Electroretinography (ERG) was performed every month after birth using the HMsERG system (Ocuscience, Las Vegas, NV) as previously described [8] (link). Briefly, after dark adapted overnight, the rats were anesthetized with an injection of ketamine/xylazine (37.5 mg/kg ketamine and 5 mg/kg xylazine, i.p.) and 0-2% isoflurane mixed with oxygen through a gas anesthesia mask (Stoelting, Wood Dale, IL, USA); and eyes were dilated using tropicamide 1% (Bausch & Lomb Inc., Tampa, FL) eye drops. Contact lens electrodes were placed on the cornea of both eyes, with reference and ground electrodes placed subcutaneously. An optically clear ophthalmic gel was used to maintain hydration and conductivity between the cornea and recording electrodes. Scotopic testing was conducted with flash stimuli intensities ranging from 1 to 25,000 millicandela (mcd) followed by photopic testing (light adaptation of 10 min prior to the photopic test which records flash stimuli responses of 10-25,000 mcd).
Tropicamide 1
Manufactured by Bausch & Lomb
Sourced in United States
Tropicamide 1% is a mydriatic and cycloplegic agent used in ophthalmological procedures. It is a short-acting pharmacological agent that induces pupil dilation and temporary paralysis of the ciliary muscle responsible for accommodation.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using tropicamide 1
1
Electroretinography Monitoring of Rat Visual Function
2
Comprehensive Ophthalmic Examinations in Restrained Animals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete ophthalmic examinations under manual restraint were also performed at the initiation of the study. One drop of Tropicamide 1% (Bausch & Lomb Inc., Tampa, FL) was administered into each eye prior to the onset of the exam. The examinations included a neuro-ophthalmic examination, fundic examination, tonometry, slit lamp biomicroscopy (Kowa-SL2, Kowa, Tokyo, Japan), and corneal pachymetry. Tonometry was performed with a rebound tonometer (Tonovet, Icare Finland Oy, Espoo, Finland). The rebound tonometer has three available calibration settings: canine, equine, and “other”: other is used for species for which a calibration table has not been established. The calibration setting for “other” was used in this study. A corneal pachymeter (Compuscan P ultrasonic pachymeter, 20 MHz, Strorz Instrument Company, St. Louis, Missouri) was used in the exam as a gauge of corneal thickness. Pachymetry readings were taken in triplicate for each eye during the exam. Serial ophthalmic examinations were performed at two-month intervals, and at the end of the study period, with the same study procedure repeated for each examination period. A single, blinded investigator (ALL) performed all of the ophthalmic exams.
3
Comprehensive Eye Examination Protocol for Glaucoma Suspects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Participants were notified to schedule a comprehensive eye examination at the Kimmel Center for the Performing Arts (visit 2) when they were diagnosed as glaucoma suspects, ocular hypertension, any other eye pathology or having poor quality of optic disc photos from visit 1. Comprehensive eye examinations included: 1) Snellen BCVA measurement, 2) central corneal thickness measurement using an iPac Handheld Pachymeter (Reichert Inc, Depew, NY, USA), and 3) VF testing using Octopus 300 Static Perimetry (Haag-Streit Inc, Bern, Switzerland). A glaucoma specialist performed the following: 1) slit lamp examination of the anterior segment, 2) gonioscopy, 3) IOP measurement using Goldmann applanation tonometry, and 4) undilated fundus examination. If deemed medically necessary and permitted by the study participant, dilation was performed using tropicamide 1% (Bausch and Lomb Inc., Tampa, FL, USA) and phenylephrine 2.5% (Akorn Inc., Lake Forest, IL, USA). Glaucoma was considered present if the participant exhibited glaucomatous optic neuropathy and characteristic VF loss in either eye. Participants were diagnosed with glaucoma, suspicion of glaucoma, ocular hypertension, or no glaucomatous findings in either eye. If treatment or follow-up was needed, participants were referred to an ophthalmologist locally or at Wills Eye Hospital.
4
Electroretinography and Scotopic Testing in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Electroretinography was performed within 1 month prior to the SC recording (age at testing ranged between 2.5 and 6 months) using the HMsERG system (Ocuscience, Las Vegas, NV, USA) as previously described.45 ,46 (link) Briefly, rats were dark adapted for a minimum of 2 hours prior to testing. The rats were then anesthetized with ketamine/xylazine and 0.5% to 2% isoflurane. Pupils were dilated using tropicamide 1% (Bausch & Lomb, Inc.) eye drops. Contact lens electrodes were placed on the cornea of both eyes, with reference and ground electrodes placed subcutaneously. An optically clear ophthalmic gel was used to maintain hydration and conductivity between the cornea and recording electrodes. Scotopic testing was conducted with flash stimuli intensities ranging from 1 to 25,000 millicandela (mcd) followed by photopic testing (light adaptation of 10 minutes prior to the photopic test, which records flash stimuli responses of 10–25,000 mcd).
5
Ocular and Systemic Measurements in Healthy Participants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The study followed the guidelines of the Declaration of Helsinki and was approved by the Aston Optometry and Audiology Research Ethics Committee. All participants gave written informed consent prior to inclusion in the study. We included 45 arbitrarily selected eyes (25 left and 20 right eyes) of 45 healthy participants (age range: 21 – 62 years). Twelve subjects were of South Asian (SA) background and thirty-three of Caucasian (CA) origin. All participants initially underwent non-contact tonometry (Pulsair, Keeler, UK) followed by pupil dilation using one drop of Tropicamide 1 % (Bausch & Lomb, UK). After a minimum of 15–20 min acclimatisation in a temperature controlled room (21 ± 2 °C) systemic blood pressure and heart rate was measured using a digital sphygmomanometer (UA-767, A&D Instruments Ltd., UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!