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Anti pan cytokeratin mouse monoclonal antibody

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The Anti-pan-cytokeratin mouse monoclonal antibody is a laboratory reagent designed for the detection and identification of cytokeratin proteins. Cytokeratins are intermediate filament proteins found in the intracytoplasmic cytoskeleton of epithelial cells. This antibody can be used to label a wide range of epithelial cell types.

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Lab products found in correlation

Bovine serum albumin (bsa), by Corning (2 mentions) Normal goat serum (ngs), by Jackson ImmunoResearch (2 mentions) Dapi for nuclear counterstaining, by Thermo Fisher Scientific (2 mentions) Qdot 800, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647, by Thermo Fisher Scientific (2 mentions)

2 protocols using anti pan cytokeratin mouse monoclonal antibody

1

Multicolor Immunostaining of CTC Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enriched CTC cell preparations were plated on coverslips and fixed with pre-warmed (37 °C) 2% formaldehyde (Sigma-Aldrich, St. Louis, MO) in 1× PHEM buffer (60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl2) for 15 min and blocked overnight in 10% Normal Goat Serum (Jackson ImmunoResearch, West Grove, PA) plus 6% Bovine Serum Albumin (BSA) in PBS (Corning, Tewksbury, MA).
Cells were subsequently immunostained for: (1) the leukocyte marker CD45 using an anti-CD45 antibody directly conjugated with QDot-800 (Invitrogen, Waltham, MA Catalog # Q10156); (2) the epithelial marker cytokeratin, using an anti-pan-cytokeratin mouse monoclonal antibody recognizing human cytokeratins 4, 5, 6, 8, 10, 13, and 18 (BioLegend, San Diego, CA catalog # 628602) conjugated to CF594 using Biotium Mix-n- Stain antibody labeling kit (catalog # 92236); (3) tubulin (Novus rat anti-tyrosinated tubulin, Catalog # NB600-506), followed by goat anti-rat IgG secondary antibody, AlexaFluor 647 (Thermo Fisher Scientific, catalog # A21247); (4) androgen receptor AR [Rabbit anti-AR (N-terminal) catalog # ab3510], and goat anti-rabbit IgG secondary antibody, AlexaFluor 488 (Catalog # A11034); and (5) DAPI for nuclear counterstaining (Invitrogen). Coverslips were mounted on a glass slide by using Mowiol mounting media (Electronic Microscopy Sciences, Hatfield, PA).
Mukhtar E., Worroll D., Galletti G., Schuster S., Piha-Paul S.A, & Giannakakou P. (2020). Quantitative analysis of taxane drug target engagement of microtubules in circulating tumor cells from metastatic castration resistant prostate cancer patients treated with CRXL301, a nanoparticle of docetaxel. Cancer Drug Resistance, 3(3), 636-646.
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2

Multicolor Immunostaining of CTC Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enriched CTC cell preparations were plated on coverslips and fixed with pre-warmed (37 °C) 2% formaldehyde (Sigma-Aldrich, St. Louis, MO) in 1× PHEM buffer (60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl2) for 15 min and blocked overnight in 10% Normal Goat Serum (Jackson ImmunoResearch, West Grove, PA) plus 6% Bovine Serum Albumin (BSA) in PBS (Corning, Tewksbury, MA).
Cells were subsequently immunostained for: (1) the leukocyte marker CD45 using an anti-CD45 antibody directly conjugated with QDot-800 (Invitrogen, Waltham, MA Catalog # Q10156); (2) the epithelial marker cytokeratin, using an anti-pan-cytokeratin mouse monoclonal antibody recognizing human cytokeratins 4, 5, 6, 8, 10, 13, and 18 (BioLegend, San Diego, CA catalog # 628602) conjugated to CF594 using Biotium Mix-n- Stain antibody labeling kit (catalog # 92236); (3) tubulin (Novus rat anti-tyrosinated tubulin, Catalog # NB600-506), followed by goat anti-rat IgG secondary antibody, AlexaFluor 647 (Thermo Fisher Scientific, catalog # A21247); (4) androgen receptor AR [Rabbit anti-AR (N-terminal) catalog # ab3510], and goat anti-rabbit IgG secondary antibody, AlexaFluor 488 (Catalog # A11034); and (5) DAPI for nuclear counterstaining (Invitrogen). Coverslips were mounted on a glass slide by using Mowiol mounting media (Electronic Microscopy Sciences, Hatfield, PA).
Mukhtar E., Worroll D., Galletti G., Schuster S., Piha-Paul S.A, & Giannakakou P. (2020). Quantitative analysis of taxane drug target engagement of microtubules in circulating tumor cells from metastatic castration resistant prostate cancer patients treated with CRXL301, a nanoparticle of docetaxel. Cancer Drug Resistance, 3(3), 636-646.
+ Open protocol
+ Expand

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