Novex nupage 4 12 gradient bis tris gels with mops sds running buffer

Manufactured by Thermo Fisher Scientific

The Novex NuPAGE 4–12% gradient bis-tris gels with MOPS SDS running buffer are pre-cast polyacrylamide gels designed for the separation of proteins. The gels feature a 4-12% gradient of acrylamide, providing effective separation of a wide range of protein molecular weights. The MOPS SDS running buffer is used in conjunction with the gels to facilitate protein electrophoresis.

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Lab products found in correlation

Pcr cleanup kit, by Qiagen (2 mentions) Plasmid miniprep kit, by Qiagen (2 mentions) High fidelity kod hot start dna polymerase, by Merck Group (2 mentions) Trypsin gold, by Promega (2 mentions) Novex colloidal blue staining kit, by Thermo Fisher Scientific (2 mentions) Krypton fluorescent protein stain, by Thermo Fisher Scientific (2 mentions) Complete hl 5 and defined sih arginine lysine growth media, by Formedium (2 mentions) Unlabelled l arginine, by Formedium (2 mentions) L lysine, by Formedium (2 mentions) Labelled k8 l lysine, by Cambridge Isotopes (2 mentions) R6 l arginine, by Cambridge Isotopes (2 mentions) Ziptip c 18 peptide purification microcolumns, by Merck Group (2 mentions) Nunc lab tek chambered cover glass plates for cell imaging, by Thermo Fisher Scientific (2 mentions) Turbo dnase, by Thermo Fisher Scientific (2 mentions) Antarctic phosphatase, by New England Biolabs (2 mentions) Complete edta free protease inhibitor cocktail, by Roche (1 mentions) Complete edta free protease inhibitors cocktail, by Roche (1 mentions)

Spelling variants of this product

NuPAGE Novex 4–12% Bis-Tris gels (322 citations) NuPAGE 4–12% Bis-Tris gradient gels (139 citations) NuPAGE-Novex Bis-Tris 4%–12% gradient gels (14 citations) NuPAGE Novex 4–12% Bis-Tris Gradient Gel (14 citations) NuPAGE Novex Bis-Tris gradient gels (7 citations) NuPAGE 4%-12% Bis-Tris SDS gels (6 citations) Novex 4-12% Bis-Tris gradient gels (5 citations) SDS NuPAGE Bis-Tris 4–12% gels (4 citations) Novex NuPAGE Bis-Tris SDS 4-12% gel (2 citations) Novex NuPAGE MOPS SDS running buffer (2 citations)

2 protocols using novex nupage 4 12 gradient bis tris gels with mops sds running buffer

Proteome Analysis of SIH Cell Lines

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Complete HL-5 and defined SIH (-arginine; -lysine) growth media and unlabelled L-arginine and L-lysine were from Formedium. Labelled K8 L-lysine and R6 L-arginine were from Cambridge Isotope Laboratories. Complete EDTA-free protease inhibitor cocktail was from Roche. Turbo DNase was from Ambion. Novex NuPAGE 4–12% gradient bis-tris gels with MOPS SDS running buffer and Novex colloidal blue staining kit were from Invitrogen. Trypsin Gold was from Promega. LavaPep peptide quantification kit was from Gel Company. ZipTip C-18 peptide purification microcolumns and high fidelity KOD hot start DNA polymerase were from Merck Millipore. Restriction enzymes, T4 DNA ligase and Antarctic phosphatase were from New England Biolabs. Plasmid miniprep kit and PCR clean-up kit were from Qiagen. Krypton fluorescent protein stain and Nunc Lab-Tek chambered cover glass plates for cell imaging were from Thermo Scientific. All the other reagents were obtained from Sigma-Aldrich.

Sobczyk G.J., Wang J, & Weijer C.J. (2014). SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale. Nature Communications, 5, 3319.

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Quantitative Mass Spectrometry Proteomics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Complete HL-5 and defined SIH (-arginine; -lysine) growth media and unlabelled L-arginine and L-lysine were from Formedium. Labelled K8 L-lysine and R6 L-arginine were from Cambridge Isotope Laboratories. Complete EDTA-free Protease Inhibitors cocktail was from Roche. Turbo DNase was from Ambion. Novex NuPAGE 4-12% gradient bis-tris gels with MOPS SDS running buffer and Novex colloidal blue staining kit were from Invitrogen. Trypsin Gold was from Promega. LavaPep peptide quantification kit was from Gel Company. ZipTip C-18 peptide purification microcolumns and high fidelity KOD hot start DNA polymerase were from Merck Millipore. Restriction enzymes, T4 DNA ligase and Antarctic Phosphatase were from New England Biolabs. Plasmid miniprep kit and PCR clean-up kit were from Qiagen. Krypton fluorescent protein stain and Nunc Lab-Tek chambered cover glass plates for cell imaging were from Thermo Scientific. All the other reagents were obtained from Sigma Aldrich.

Sobczyk G.J., Wang J, & Weijer C.J. (2014). SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale. Nature Communications, 5, 3319.

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