Tr 35 ms

The samples were analyzed for the detection of compounds through Gas Chromatography- Mass Spectroscopy (GC-MS). The compounds were separated by using (GC, Trace-1300, Thermo scientific) and capillary column (TR-35 MS) with (30 m x 0.25 mm I.D. x 0.25 μm) dimensions. For the separation of compounds Helium gas was used. The temperature of the injection port was maintained at 250°C while 1 μL of the sample volume was injected for compound separation. The initial temperature of column was maintained at 50°C for 1 minute, and then programmed at 20°C/min to 300°C for 38 min. The known compounds stored in the GC-MS library and software data system were used for comparison of the results spectrums of separated compounds.

The extract was analysed on a gas chromatography-tandem mass spectrometry instrument (TSQ Quantum XLS, Thermo Scientific, USA). Chromatography separation was achieved using a GC-column, TR-35MS (30 m x 0.25 mm x 0.25 μm film thickness, Thermo Scientific). One microliter of diluted sample was injected in splitless mode, and the injector temperature was set at 220°C. The carrier gas was high-purity helium (99.999%) and the flow rate was 1.2 mL/min. The oven temperature was initially maintained at 60°C for 3 min, then increased to 200°C at 30°C/min, then to 300°C at 5°C/min, and held for 5 min (the total run time was 36.2 min). The transfer line temperature was 250°C. The conditions of the mass detector were as follows: ionisation mode, electron impact (EI) at 70 eV, full scan range 35–1000 m/z, the ion source temperature was 250°C. Natural compounds in all samples were processed using Xcalibur software and were identified using the NIST 14 database.

Before putting the samples into a Thermo Trace GC UltraTM gas chromatograph system (Thermo Scientific, USA), they were concentrated with a gentle stream of clean nitrogen. PAHs were measured using a fused silica capillary column, Thermo TR-35 MS, with 35% phenyl polysil-phenylene-siloxane. The temperature went from 90 to 140 °C at a rate of 5 °C per minute and stayed there for 1 min. The temperature was then raised from 140 to 250 °C at a rate of 3 °C per minute and held there for 1 min. The temperature was then raised from 250 to 300 °C at a rate of 20 °C per minute and held there for 1 min. The temperature of the detector and injector was set to 310 °C and 280 °C, respectively.