Cd19 buv737

Manufactured by BD

Sourced in Czechia

CD19-BUV737 is a fluorochrome-conjugated monoclonal antibody that binds to the CD19 cell surface antigen. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Cd8 buv395, by BD (2 mentions) Cd45 v450, by BD (2 mentions) Cd34 bv786, by BD (2 mentions) Cd38 pe, by Exbio (2 mentions) Cd4 buv395, by BD (2 mentions) Histopaque 1077, by Merck Group (2 mentions) Cd123 buv395, by BD (1 mentions) Cd11c pe cy7, by BD (1 mentions) Cd11b alexa fluor 647, by BD (1 mentions) Streptavidin qdot605, by Thermo Fisher Scientific (1 mentions) Cd45 buv395, by BD (1 mentions) Pd l2 apc, by Thermo Fisher Scientific (1 mentions) Hla dr fitc, by Thermo Fisher Scientific (1 mentions) Tim3 apc, by Thermo Fisher Scientific (1 mentions) Lightning link fluorescein conjugation kit, by Abcam (1 mentions) Ccr2 fitc, by BioLegend (1 mentions) Cd115 bv421, by BioLegend (1 mentions) Ly6g pe cf594, by BD (1 mentions) Cd11b pe, by Thermo Fisher Scientific (1 mentions) Lsr 2 fortessa flow cytometer, by Tree Star (1 mentions)

4 protocols using cd19 buv737

Immunophenotyping of AML Leukapheresis Samples

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Mononuclear cells from the peripheral blood of 36 patients with AML indicated at leukapheresis due to hyperleukocytosis at diagnosis were obtained by separation of leukapheretic products on Histopaque 1077 (Sigma, Prague, Czech Republic). The cells were resuspended in RPMI 1640 medium with 10% fetal calf serum and with antibiotics (100 U/mL penicillin, 100 µg/mL streptomycin), and aliquots were used for RNA isolation and for analysis of surface markers by flow cytometry. Additional analyses of PD-L1 transcripts were performed from cDNA samples, which had been taken for routine clinical analyses and cryopreserved. A written informed consent with the use of biological material for research purposes was obtained from all patients. The study was approved by the Ethics Committee of the Institute of Hematology and Blood Transfusion of the Czech Republic as a part of the research project 16-30268A (June 2015).
Antibodies used were the following: CD45-V450 (#560367), CD4-BUV395 (#564724), CD8-BUV395 (#563795), CD19-BUV737 (#564303), CD123-BUV395 (#564195), CD371(CLL-1)-BB515 (#565926), CD135(FLT3)-AlexaFluor647 (#563494), and CD34-BV786 (#743534) were from BD Biosciences (Prague, Czech Republic); CD38-PE (#1P-366-T100) from Exbio (Prague, Czech Republic); PD-1-APC (#17-2799-42) and PD-L1-PE-Cy7 (#25-5983-42) from Affymetrix, Inc. (San Diego, CA, USA).

Brodská B., Otevřelová P., Šálek C., Fuchs O., Gašová Z, & Kuželová K. (2019). High PD-L1 Expression Predicts for Worse Outcome of Leukemia Patients with Concomitant NPM1 and FLT3 Mutations. International Journal of Molecular Sciences, 20(11), 2823.

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Multicolor Flow Cytometry of Islet Immune Cells

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Islets were isolated from NOD.CD11c-DTR-GFP mice as previously described. Islet samples were stained with the fluorescent-labeled antibodies CD45 BUV395 (BD), CD90.2 BUV737 (BD), CD19 BUV737 (BD), CD11c PE-Cy7, CD11b Alexa Fluor 647, XCR1 BV785, MHC-II Biotin (BD), Streptavidin Qdot605 (Invitrogen), rabbit α-MERTK (Abcam), and donkey α-rabbit BV421 (BioLegend).

Lindsay R.S., Whitesell J.C., Dew K.E., Rodriguez E., Sandor A.M., Tracy D., Yannacone S.F., Basta B.N., Jacobelli J, & Friedman R.S. (2021). MERTK on mononuclear phagocytes regulates T cell antigen recognition at autoimmune and tumor sites. The Journal of Experimental Medicine, 218(10), e20200464.

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Phenotyping Leukemic Cells from AML Patients

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Primary cells from peripheral blood of AML patients with hyperleukocytosis were obtained by leukapheresis before therapy initiation. The leukapheretic products were diluted 10-fold in phosphate buffered saline (PBS), and the mononuclear cell fraction was separated using Histopaque-1077 (Sigma, #H8889). The cells were resuspended in RPMI 1640 medium with 10% fetal calf serum and with antibiotics (100 U/ml penicillin, 100 µg/ml streptomycin), and aliquots were used for analysis of surface markers by flow cytometry and for mRNA isolation.
The antibodies used were as follows: CD45-V450 (#560367), CD4-BUV395 (#564724), CD8-BUV395 (#563795), CD19-BUV737 (#564303), CD34-BV786 (#743534), and CD371-BB515 (#565926) from BD Biosciences; HLA-DR-FITC (#11-9952-42), TIM-3-APC (#17-3109-42), and PD-L2-APC (#17-5888-42) from eBioscience; CLIP-PE (sc-12725 PE) from Santa Cruz; PD-L1-PE (#1P-177-T100), CD47-FITC (#1F-225-T100), and CD38-PE (#1P-366-T100) from Exbio (Prague, Czech Republic). HLA class I antibody (Abcam, ab2217) was conjugated in house using the Lightning-Link Fluorescein Conjugation kit (#707-0010, Innova Biosciences).

Kuželová K., Brodská B., Marková J., Petráčková M., Schetelig J., Ransdorfová Š., Gašová Z, & Šálek C. (2022). NPM1 and DNMT3A mutations are associated with distinct blast immunophenotype in acute myeloid leukemia. Oncoimmunology, 11(1), 2073050.

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Isolation and Characterization of Peritoneal Immune Cells

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Peritoneal exudate cells (PECs) were isolated via lavage and single cell suspensions were briefly washed with PBS prior to red blood cell lysis using ACK lysis buffer. Prepared cells were stained with Zombie Yellow (BioLegend) to assess live status of cells. The following antibodies were used, though not necessarily all in the same panel: F4/80- BV785 (BioLegend; Clone BM8), CD115-BV421 (BioLegend; Clone AFS98), CD11c-BV421 (BD Bioscience; Clone HL3), CD115-BUV395 (BD Bioscience; Clone AFS98), CD19-BUV737 (BD Bioscience; Clone 1D3), CD226-PE (BD Bioscience; Clone TX42.1), CD11b-PE (eBioscience; Clone M1/70), MHCII (I-A/I-E)- PE/Cy7 (BioLegend; Clone M5/114.15.2), Ly6G-PE/CF594 (BD Bioscience; Clone 1A8), Ly6C-APC/eFluor780 (eBiosceince; Clone HK1.4), CD102-Alexa Fluor 647 (BioLegend; Clone 3C4 (mlC2/4)), CD102-Alexa Fluor 488 (eBioscience; Clone 3C4 [mlC2/4]), CCR2-FITC (BioLegend; Clone SA203G11), and CD11b-PErCP/Cy5.5 (BioLegend; Clone M1/70). Antibody cocktails were prepared in FACs staining buffer (phosphate-buffered saline, 1% FBS, 1% 0.5mM EDTA). Cellular fluorescence was measured using an LSRII Fortessa flow cytometer, and data were analyzed using FlowJo software (Tree Star).

Martin A.T., Giri S., Safronova A., Eliseeva S.I., Kwok S.F, & Yarovinsky F. (2024). Parasite-induced IFN-γ regulates host defense via CD115 and mTOR-dependent mechanism of tissue-resident macrophage death. PLOS Pathogens, 20(2), e1011502.

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