Serum samples were collected from fresh blood. Levels of interferon-γ (IFN-γ), interleukin-2 (IL-2), IL-4 and IL-10 were measured using Th1/Th2 ELISA kit (catalogue # 88-7711-44; affymetrix eBioscience, California, USA) following the procedure in the catalogue.
Th1 th2 elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States, Canada, United Kingdom
The TH1/TH2 ELISA Kit is a laboratory tool used for the quantitative determination of T-helper 1 (Th1) and T-helper 2 (Th2) cytokines in biological samples. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the levels of specific cytokines, which are important markers for immune system function and response.
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5 protocols using th1 th2 elisa kit
1
Cytokine Profiling in Serum Samples
2
Cytokine Profiling of Murine Splenocytes Exposed to Herbal Extracts
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Murine splenocytes (2 10⁶ cells/mL) were cultured with various extracts of H. orientalis L. (50 mg/mL) for 48 h in CO₂ incubator at 37 °C. Levels of IFN-γ, IL-2, IL-4, and IL-10 were measured using TH1/TH2 ELISA kit (affymetrix ebioscience, Canada). After period of incubation, supernatants were collected and used to estimate the level of cytokines according to the standard kit procedure [46 (link)]. Based on the calculated standard curve of each cytokine, absorbance values were converted into concentration (pg/mL).
3
Cytokine Profiling in Bladder Infection
4
Cytokine Profiling in Bladder Infection
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Bladders were collected at different time point post infection as indicated in the figure legends. Then bladders were homogenized by using zirconia silica beads for three cycles of 1.5 min each in an automatic homogenizer, and enzyme-linked immunosorbent assay was performed by using TH1/TH2 ELISA Kit (88–7711-44, ThermoFisher), IL-12p70 ELISA Kit (BMS6004, ThermoFisher), EGF ELISA Kit (EMEGF, ThermoFisher), IGF-1 ELISA Kit (EMIGF1, ThermoFisher), or TGFα ELISA Kit (EHTGFA, ThermoFisher) following manufacture’s instruction. Concentrations were determined by standard curve. The level of IFN-γ and IL-4 at various time points was normalized to the level at day 0 without infection.
5
Cytokine Profiling of Murine Splenocytes Exposed to Rice Bran Extracts
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In this assay, cytokines (IFN-γ, IL-2, IL-4, and IL-10) were measured for murine splenocytes cultured for 48 h with various extracts of rice bran using mouse TH1/TH2 ELISA kit. In particular, the cell suspension (splenocytes in RPMI-1640 medium) was seeded onto a 96-well plate at a concentration of 2 × 10⁶ cells/mL, along with 100 µL/well of different rice bran extracts (5 mg/mL). Following 48 h of incubation, culture supernatants were isolated to measure the level of cytokines according to the technical procedure mentioned in TH1/TH2 ELISA kit (Catalogue no. 88-7711-44) (Thermo Fisher Scientific, Oxford, UK). To estimate the concentration of each cytokine, a standard curve was prepared for each one. The measured absorbance values were converted into concentration (pg/mL) using standard curve.
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