Glycogen periodic acid schiff pas hematoxylin stain kit

Manufactured by Solarbio

Sourced in China

The Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain Kit is a laboratory reagent used for the detection and visualization of glycogen in tissue samples. The kit contains the necessary reagents to perform the PAS staining technique, which utilizes periodic acid to oxidize glycol groups, and the Schiff reagent to produce a magenta-colored stain. The kit also includes hematoxylin for counterstaining nuclei. This staining method is commonly used in histological and pathological analyses.

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Lab products found in correlation

Infinite m200, by Tecan (1 mentions) Trypsin, by Solarbio (1 mentions) Evos fl auto cell imaging system, by Thermo Fisher Scientific (1 mentions)

7 protocols using glycogen periodic acid schiff pas hematoxylin stain kit

Glycogen Periodic Acid Schiff Staining

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The PAS staining was performed following Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain Kit (Solarbio, China) protocol 17 (link).

Du X., Yang Y., Yang M., Yuan L., Wang L., Wu M., Zhou K., Li W., Xiang Y., Qu X., Liu H., Qin X, & Liu C. (2022). ITGB4 deficiency induces mucus hypersecretion by upregulating MUC5AC in RSV-infected airway epithelial cells. International Journal of Biological Sciences, 18(1), 349-359.

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Glycogen Quantification Using PAS Stain

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Glycogen was measured using a Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain Kit (Solarbio-G1281, Beijing) and the assay was performed according to the manufacturer’s instructions.

Xu L., Jois S, & Cui H. (2022). Metformin and Gegen Qinlian Decoction boost islet α-cell proliferation of the STZ induced diabetic rats. BMC Complementary Medicine and Therapies, 22, 193.

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Intestinal Permeability Evaluation Protocol

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The intestinal permeability was determined by quantifying mucus-containing goblet cells and the level of the tight junction proteins (ZO-1 and occludin) expressed in the intestine. The quantity of mucus-containing goblet cells was detected by periodic acid-Schiff staining. The isolated intestine was dehydrated in a graded ethanol series, cleared in xylene, and embedded in paraffin. The intestinal paraffin blocks were cut into sections 4 μm thick with a paraffin-slicing microtome and then stained with glycogen periodic acid-Schiff (PAS/hematoxylin) stain kit (Solarbio, Beijing, China). Goblet cells were observed by optical microscopy and counted by using Image J software. The tight junction proteins (ZO-1 and occludin) expressed in the intestine were assessed by Western blotting.

Wu Y., Dong Z., Jiang X., Qu L., Zhou W., Sun X., Hou J., Xu H, & Cheng M. (2023). Gut Microbiota Taxon-Dependent Transformation of Microglial M1/M2 Phenotypes Underlying Mechanisms of Spatial Learning and Memory Impairment after Chronic Methamphetamine Exposure. Microbiology Spectrum, 11(3), e00302-23.

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Glycogen Content Quantification and Visualization

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A Glycogen Content Detection Kit (Beijing Solarbio Science & Technology, China) was used to detect glycogen content in cells and liver tissues according to the manufacturer’s protocol. Absorbance was measured using a microplate fluorimeter (Infinite M200; Tecan, Hillsborough, NC). A Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain Kit (Beijing Solarbio Science & Technology, China) was used to display glycogen levels in liver tissue. PAS staining was observed using a light microscope (Olympus).

Long Z., Fan J., Wu G., Liu X., Wu H., Liu J., Chen Y., Su S., Cheng X., Xu Z., Su H., Cao M., Zhang C., Hai C, & Wang X. (2020). Gestational bisphenol A exposure induces fatty liver development in male offspring mice through the inhibition of HNF1b and upregulation of PPARγ. Cell Biology and Toxicology, 37(1), 65-84.

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Retinal Trypsin Digestion for Vascular Assessment

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Retinal trypsin digestion was performed following a previously described procedure [27] (link), with some modifications. The eyes of anesthetized rats were enucleated and fixed overnight with 10% neutral buffered formalin. The retinas were dissected and gently shaken in water at room temperature overnight, then digested with 3% trypsin (1:250; Solarbio, Beijing, China) at 37 °C for 3 h. After repeated washing, the network of vessels was isolated and mounted on slides. The dried vessels were stained with the Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain Kit (Solarbio). At least ten different fields in each retina were randomly chosen and the number of acellular capillaries was counted in each field under the microscope (Olympus DP80; Olympus, Tokyo, Japan).

Zhu K., Hu X., Chen H., Li F., Yin N., Liu A.L., Shan K., Qin Y.W., Huang X., Chang Q., Xu G.Z, & Wang Z. (2019). Downregulation of circRNA DMNT3B contributes to diabetic retinal vascular dysfunction through targeting miR-20b-5p and BAMBI. EBioMedicine, 49, 341-353.

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Glycogen Quantification via PAS Staining

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PAS glycogen staining was performed using the Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain Kit (Solarbio, Beijing, China), according to the manufacturer’s protocol. Slides were photographed with the EVOS FL Auto Cell Imaging System (Thermo Fisher Scientific, Waltham, MA, USA), and PAS quantification was performed using Image-Pro Plus software.

Yang L., Cheng X., Shi W., Li H., Zhang Q., Huang S., Huang X., Wen S., Gan J., Liao Z., Sun J., Liang J., Ouyang Y, & He M. (2022). Vasorin Deletion in C57BL/6J Mice Induces Hepatocyte Autophagy through Glycogen-Mediated mTOR Regulation. Nutrients, 14(17), 3600.

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Liver Glycogen Quantification via PAS Stain

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Liver glycogen was measured using a Glycogen Periodic Acid Schiff(PAS/Hematoxylin) Stain Kit (G1281; Solarbio, Beijing, China) and the assay was performed according to the manufacturer’s instructions.

Xu L., Hu R., Jois S.V, & Zhang L. (2023). Oleanolic acid and moderate drinking increase the pancreatic GLP-1R expression of the β-cell mass deficiency induced hyperglycemia. PeerJ, 11, e15705.

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