Automatic biochemistry analyzer

Manufactured by Roche

Sourced in United States, Switzerland, Germany

The Automatic Biochemistry Analyzer is a laboratory instrument designed to perform automated analysis of various biochemical components in samples. It is used to measure the levels of substances such as enzymes, proteins, lipids, and other metabolites in biological fluids like blood, urine, or other bodily samples. The core function of this analyzer is to provide accurate and reliable quantitative results to support clinical diagnostic and research activities.

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Spelling variants of this product

Automatic analyzer (18 citations) Modular P800 Automatic Biochemistry Analyzer (5 citations) Fully automatic biochemistry analyzer (2 citations) Cobas 8000 automatic biochemistry analyzer (2 citations) Cobas automatic biochemistry analyzer (2 citations)

20 protocols using automatic biochemistry analyzer

Serum and Urinary Biomarkers in Diabetic Rats

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We collected blood samples to assess serum lipid and renal and hepatic function as previously reported (Ma et al., 2015 (link)) before STZ injection and after saxagliptin treatment. At the end of the study, all rats were placed in individual metabolic cages to collect 24-h urine samples for measurement of urinary microalbumin (UMA) excretion and urinary chemistries. Urinary UMA was examined with an automatic biochemistry analyzer (Roche). The urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) and mindin levels were measured with commercially available enzyme-linked immunosorbent assay (ELISA) kits (Biotopped, Beijing, China). Rats were sacrificed under anesthesia by intraperitoneal injection of chloral hydrate. Blood samples were obtained from the retroorbital venous plexus at sacrifice and were centrifuged at 3000 rpm/min for 10 min. Total triglyceride (TG), total cholesterol (TC), blood urea nitrogen (BUN), serum creatinine (Scr), aspartate transaminase (AST), and alanine transaminase (ALT) in serum were tested with an automatic biochemistry analyzer (Roche). Then, the left kidneys were immediately removed, weighed, and frozen in liquid nitrogen before being stored at -80°C or fixed in 4% paraformaldehyde.

Chang Y.P., Sun B., Han Z., Han F., Hu S.L., Li X.Y., Xue M., Yang Y., Chen L., Li C.J, & Chen L.M. (2017). Saxagliptin Attenuates Albuminuria by Inhibiting Podocyte Epithelial- to-Mesenchymal Transition via SDF-1α in Diabetic Nephropathy. Frontiers in Pharmacology, 8, 780.

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Serum Biochemical Marker Quantification

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Corresponding commercial kits, purchased from Sino-German Beijing Leadman Biotech Ltd. (Beijing, China), and an automatic biochemistry analyzer (Roche, Basel, Switzerland) instrument were used to measure the concentrations of serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate amino transferase (AST), cholinesterase (CHE2), and total bile acid (TBA).

Duan G., Huang P., Zheng C., Zheng J., Yu J., Zhang P., Wan M., Li F., Guo Q., Yin Y, & Duan Y. (2023). Development and Recovery of Liver Injury in Piglets by Incremental Injection of LPS. Antioxidants, 12(6), 1143.

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Biomarker Panel in Fasting Subjects

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Blood samples of 5 mL were collected from the antecubital vein of fasting subjects by sterile disposable syringe in an aseptic manner. Sera were separated and stored at-20°C for future testing. Serum glucose, alanine aminotransferase (ALT) triglyceride (TG), total cholesterol (TCh), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) were determined by automatic biochemistry analyzer (Roche, Rotkreuz, Switzerland). Serum HBsAg and anti HCV were detected by ELISA.

Rahman M.M., Kibria M.G., Begum H., Haque M., Sultana N., Akhter M., Rowshon A.H., Ahmed F, & Hasan M. (2020). Prevalence, risk factors and metabolic profile of the non-obese and obese non-alcoholic fatty liver disease in a rural community of South Asia. BMJ Open Gastroenterology, 7(1), e000535.

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Fasting Lipid and Inflammatory Biomarkers

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Upon admission, elbow venous blood was taken from each participant, who was instructed to fast for more than 12 h prior to the blood draw. An automatic biochemistry analyzer (Roche, Switzerland) was used to measure serum levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), creatinine (Cr), and high-sensitivity C-reactive protein (hs-CRP).

Guo F., Tang C., Huang B., Gu L., Zhou J., Mo Z., Liu C, & Liu Y. (2021). LncRNA H19 Drives Proliferation of Cardiac Fibroblasts and Collagen Production via Suppression of the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β Axis. Molecules and Cells, 45(3), 122-133.

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Metabolic Assessment in Diabetic Kidney Disease

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After 12 weeks of treatment with CaD or vehicle, the mice were sacrificed under anesthesia. Blood samples were collected from the retroorbital venous plexus, and urine samples were collected from specific metabolic cages. The blood and urine samples were analyzed for fasting blood glucose (FBG), urinary albumin/creatinine (ACR), total triglyceride (TG), total cholesterol (TC), alanine transaminase (ALT), aspartate transaminase (AST), and serum creatinine (Scr) levels with an automatic biochemistry analyzer (Roche). Based on FBG, UAE, and ACR levels, all mice were diagnosed with DKD.

Wang Y., Lu Y.H., Tang C., Xue M., Li X.Y., Chang Y.P., Cheng Y., Li T., Yu X.C., Sun B., Li C.J, & Chen L.M. (2019). Calcium Dobesilate Restores Autophagy by Inhibiting the VEGF/PI3K/AKT/mTOR Signaling Pathway. Frontiers in Pharmacology, 10, 886.

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Measurement of Serum Biomarkers in Fasting Subjects

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Venous blood samples were collected from fasting subjects using sterile disposable syringes and needles in an aseptic manner. Blood samples were centrifuged (4000 rpm), and serum samples were collected and stored at −20°C until assay for serum glucose, triglyceride (TG), total cholesterol (TCh), high‐density lipoprotein cholesterol (HDL‐C), and low‐density lipoprotein cholesterol (LDL‐C) using an automatic biochemistry analyzer (Roche, Rotkreuz, Switzerland). Serum samples were also tested for Immunoglobulin G (IgG) antibody responses to an H. pylori membrane protein (MP) antigen (Hel 305 MP) using an enzyme‐linked immunosorbent assay (ELISA). A commercially available ELISA kit was used following the manufacturer's (Human, Wiesbaden, Germany) instructions.

Rahman M.M., Kibria M.G., Sultana N., Akhter M., Begum H., Haque M.A., Haque R., Sarker S.A., Ahmed F, & Hasan M. (2020). Seroprevalence of Helicobacter pylori and its association with metabolic syndrome in a rural community of Bangladesh. JGH Open: An Open Access Journal of Gastroenterology and Hepatology, 5(1), 64-72.

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Comprehensive Metabolic Profiling of Fasted Participants

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Fasting venous blood samples were collected from each participant and transferred to center laboratory in 30 minutes. Triglyceride (TG), TC, LDL-C, HDL-C, creatine kinase (CK), high sensitivity C-reactive protein (hs-CRP) and fasting blood glucose were measured by Automatic Biochemistry Analyzer (Roche, model c501, Sweden) after samples were centrifuged at 1500 g for 10 minutes. Glycosylated hemoglobin A_1c(HbA_1c) was measured in red blood cells using ion-exchange high-performance liquid chromatography (Sysmex, G8, Japan).

Yang C., Sun Z., Li Y., Ai J., Sun Q, & Tian Y. (2014). The correlation between serum lipid profile with carotid intima-media thickness and plaque. BMC Cardiovascular Disorders, 14, 181.

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Urinary Microalbuminuria and Metabolic Markers

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At the end of the treatment, 24 h urine samples from all rats were obtained for the measurement of urinary microablumin (UMA). All overnight-fasted animals were weighed and then anesthetized with an intraperitoneal injection of chloral hydrate. Blood samples were collected from the retroorbital venous plexus for biochemical analyses. Blood glucose, UMA, total triglyceride (TG), total cholesterol (TC), alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN) and serum creatinine (Scr) were assessed by an automatic biochemistry analyzer (Roche, Germany). The kidneys were immediately collected, weighed, and fixed in 10% neutral formalin or frozen in liquid nitrogen and then stored at -80°C until further experiments.

Xue M., Cheng Y., Han F., Chang Y., Yang Y., Li X., Chen L., Lu Y., Sun B, & Chen L. (2018). Triptolide Attenuates Renal Tubular Epithelial-mesenchymal Transition Via the MiR-188-5p-mediated PI3K/AKT Pathway in Diabetic Kidney Disease. International Journal of Biological Sciences, 14(11), 1545-1557.

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Uric Acid Metabolism Evaluation in Mice

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At the end of treatment, 10 mice from each group were placed in individual metabolic cages for 24 hours to collect the urine. The samples of blood were obtained from the retroorbital venous plexus. The blood and the urine were then used for analysis of SUA, urine UA with an automatic biochemistry analyzer (Roche). The fractional excretion of uric acid (FEUA) was calculated according to the formula: FEUA= (urine UA× serum creatinine) / (serum UA × urine creatinine) × 100, expressed as percentage 45 (link). Mice were sacrificed by intraperitoneal injection of chloral hydrate.

Lu Y.H., Chang Y.P., Li T., Han F., Li C.J., Li X.Y., Xue M., Cheng Y., Meng Z.Y., Han Z., Sun B, & Chen L.M. (2020). Empagliflozin Attenuates Hyperuricemia by Upregulation of ABCG2 via AMPK/AKT/CREB Signaling Pathway in Type 2 Diabetic Mice. International Journal of Biological Sciences, 16(3), 529-542.

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Comprehensive Metabolic Profile Assessment

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Peripheral venous blood (5 mL) was collected following an overnight fasting to measure aspartate aminotransferase (AST), alanine aminotransferase (ALT), r-glutamyl transpeptidase (r-GT), alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDLc) and low-density lipoprotein cholesterol (LDLc), free fatty acids (FFA), glucose (GLU), and insulin (INS). These substances were examined using an automatic biochemistry analyzer (Roche Diagnostics GmbH, Germany), and homeostasis model assessment for insulin resistance (HOMA-IR) was calculated from fasting insulin and glucose levels [20 (link)].

Li T.T., Tan T.B., Hou H.Q, & Zhao X.Y. (2018). Changes in peroxisome proliferator-activated receptor alpha target gene expression in peripheral blood mononuclear cells associated with non-alcoholic fatty liver disease. Lipids in Health and Disease, 17, 256.

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