Reduction in CFU was calculated as follows: First, for each experiment, the mean CFU/mL or biofilm of untreated control suspensions or biofilms was calculated (meancontrol). Then, CFU/mL or the biofilm of samples treated with disinfectant were subtracted from meancontrol to determine the CFU reduction of individual technical replicates (two or three depending on the biofilm model used). Finally, the mean of those individual technical replicates was calculated, resulting in one meanreduction value for each experimental condition (=GA or PAA concentration, respectively) per experiment. The final bar charts represent the mean reduction values of three or six experiments given in CFU in log10. Statistical analysis and data visualization were performed using Prism 9.0 GraphPad Software (Version 9.1.0, La Jolla, CA, USA). The Mann-Whitney-U test was performed to evaluate intra-laboratory reproducibility, assuming significant differences between the two technicians for a p value of <0.05.
Prism 9.0 software
Manufactured by GraphPad
Sourced in United States
Prism 9.0 is a GraphPad software for data analysis and scientific graphing. It is designed to help researchers, scientists, and professionals efficiently analyze, visualize, and present their data.
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Lab products found in correlation
4 protocols using prism 9.0 software
1
Disinfectant-Induced Bacterial CFU Reduction
2
Longitudinal Patient-Reported Outcomes in Hip Arthroplasty
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To ensure valid statistical analyses, data were subjected to a normality test using the Shapiro-Wilk test. Baseline characteristics were described using mean and standard deviation (SD) for continuous parameters and count (percent) for categorical parameters. A repeated measures analysis of variance (ANOVA) analysis was performed to identify significant differences in PROMs over time, meaning between baseline, and at one- and two-year follow-up. In case of non-normal distribution of data, non-parametric equivalents were used (i.e. median, interquartile range (IQR), and Friedman test). Previous published thresholds for minimal clinically important improvement (MCII) in mOHS (five points) and HOOS-PS (23 points) were used to identify the number (percent) of patients who achieved clinically relevant change.16 (link),17 (link) It should, however, be noted that follow-up completeness is a prerequisite for reliable outcome assessment.18 (link) As such, a complete case analysis was performed and patients were only included in the present study if they had complete data points at baseline, and one- and two-year follow-up. All statistical tests were performed with PRISM 9.0 GraphPad software (GraphPad, USA). All p-values < 0.05 were considered statistically significant.
3
Kinetic Characterization of BcGalB
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The hydrolytic activity of BcGalB was tested on several substrates: PNP-β-glu, ONP-β-glu, PNP-α-glu, PNP-β-xyl, PNP-α-man, PNP-β-man, PNP-β-fuc, PNP-α-fuc, PNP-α-rha, ONP-β-gal, PNP-β-gal, PNP-α-gal, PNP-α-ara and D-lactose. The enzyme was incubated in presence of 10 mM of each substrate under standard assay conditions. When lactose was used, the amount of free-glucose released upon hydrolysis was determined using D-Glucose Assay Kit (GOPOD Format, Megazyme) according to the manufacturer’s protocol. One unit (U) is defined as the amount of enzyme required to release 1 μmol of glucose per min. In order to study the kinetic parameters of the enzyme, different concentration values of ONP-β-gal (0.1 to 20 mM) and lactose (0–500 mM) were tested. The Michaelis–Menten constant (KM) and Vmax were calculated by non-linear regression analysis using GraphPad 9.0 Prism software.
4
Enzymatic Characterization of BcGalB
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The hydrolytic activity of BcGalB was tested on several substrates: PNP-β-glu, ONP-β-glu, PNP-α-glu, PNP-βxyl, PNP-α-man, PNP-β-man, PNP-β-fuc, PNP-α-fuc, PNP-α-rha, ONP-β-gal, PNP-β-gal, PNP-α-gal, PNP-α-ara and D-lactose. The enzyme was incubated in presence of 10 mM of each substrate under standard assay conditions. When lactose was used, the amount of free-glucose released upon hydrolysis was determined using D-Glucose Assay Kit (GOPOD Format, Megazyme) according to the manufacturer's protocol. One unit (U) is defined as the amount of enzyme required to release 1 µmol of glucose per min. In order to study the kinetic parameters of the enzyme, different concentration values of ONP-β-gal (0.1 to 20 mM) and lactose (0-500 mM) were tested. The Michaelis-Menten constant (K M ) and V max were calculated by non-linear regression analysis using GraphPad 9.0 Prism software.
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