Tetrahydrofuran

Dichloromethane (CH₂Cl₂), chloroform (CHCl₃, 99.94%), tetrahydrofuran (THF) (C₄H₈O, 99.94%) and ammonium hydroxide (NH₄OH, 24.06%) were received from Lach-Ner s.r.o., Neratovice, Czech Republic. α-bromoisobutyryl bromide (C₄H₆Br₂O, >98.0%), 3-thiopheneethanol (C₆H₈OS, 98.0%) and N,N,N′,N″,N″-pentamethyldiethylenetriamine (PMDETA) (C₉H₂₃N₃, >99.0%) were recieved from Tokyo Chemical Industry, Tokyo, Japan. Nitromethane (CH₃NO₂, 98+%), copper(I) bromide (CuBr, 98%), dibutyltin dilaurate (DBTDL, [CH₃(CH₂)₃]₂Sn[O₂C(CH₂)₁₀CH₃], iron(III) chloride, anhydrous (FeCl₃, 98%) and ethyl isocyanate (C₃H₅NO, 98%) were received from Alfa Aesar, Haverhill, MA, USA. Triethylamine (C₆H₁₅N, 99%), 3,4-ethylenedioxythiophene (EDOT, 99.0%) silica gel (SiO₂) and 2-hydroxyethyl acrylate (C₅H₈O₃, 97%) were received from Acros Organics, Geel, Belgium. Magnesium sulfate (MgSO₄, 99.4%) was received from VWR Chemicals, Leuven, Belgium. Acetone (C₃H₆O, 98.0%) and ethanol (C₂H₅OH, 96.0%) were received from Gram-mol, Zagreb, Croatia. Methanol (CH₃OH, 99.9%) was received from Carlo Erba Reagents, Val-de-Reuil, France and diethyl eter (C₄H₁₀O) was received from Macron Fine Chemicals, Radnor, PA, USA. All the solvents were used as received.

CuBr₂ (99.999% trace metal basis), CuBr (99.99%), 2,2’-bipyridyl (99%), oligo(ethylene glycol) methyl ether methacrylate (M_n = 300 g/mol, MeOEGMA), glycidyl methacrylate (97%, GMA), ethylenediamine (99.5%, EtDA), α-bromoisobutyryl bromide (98%, BiBB), copper sulphate (CuSO₄), sodium L-ascorbate (98%), NaN₃ (99.5%) were purchased from Sigma-Aldrich (Czech Republic). Deionized (DI) water was obtained from a Milli-Q purification system (Milli-Q gradient A10, Merck-Millipore). Tetrahydrofuran (THF, Lach-Ner, Czech Republic) was distilled under argon over sodium immediately prior use. All other organic solvents (Lach-Ner, Czech Republic) of analytical grade were used as received. ATRP initiators 11-(2-bromo-2-methyl)propionyloxy)undecyltrichlorosilane and ω-mercaptoundecyl bromoisobutyrate were synthesized according to procedures reported earlier [49 (link),50 (link)]. Human blood plasma, Endothelial Cell Growth Medium 2 Kit, and HUVEC cells were obtained from Promocell (Germany). Penicillin-streptomycin (10 U/mL, 10 μg/mL), trypsin-EDTA (0.05%–0.02%) and Phalloidin–Atto 594 were from Sigma-Aldrich (Czech Republic). LIVE/DEAD™ Viability/Cytotoxicity Kit for mammalian cells and Hoechst 33342 were purchased from Thermo Fisher Scientific (USA).