Ammonium chloride

Manufactured by Nacalai Tesque

Sourced in Japan

Ammonium chloride is a chemical compound with the formula NH4Cl. It is a white crystalline solid that is commonly used as a laboratory reagent. Ammonium chloride has the core function of serving as a source of ammonium ions (NH4+) and chloride ions (Cl-) in various chemical reactions and analytical procedures.

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Lab products found in correlation

Sodium iodate, by Nacalai Tesque (2 mentions) Sodium nitrite, by Nacalai Tesque (2 mentions) Sodium nitrate, by Nacalai Tesque (2 mentions) Anti β actin antibody a5441, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Sodium bromate, by Nacalai Tesque (1 mentions) Sodium iodide, by Nacalai Tesque (1 mentions) Sodium thiocyanate, by Nacalai Tesque (1 mentions) 3 trimethoxysilylpropyl methacrylate, by Tokyo Chemical Industry (1 mentions) Sodium bromide, by Nacalai Tesque (1 mentions) Fused silica capillary tube, by GL Sciences (1 mentions) Simplicity uv water purification system, by Merck Group (1 mentions) 2 methacryloyloxy ethyl dimethyl 3 sulfopropyl ammoniumhydroxide, by Merck Group (1 mentions)

5 protocols using ammonium chloride

Isolation of Murine Lymphoid Tissues

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MLN and spleen tissues were harvested from mice after euthanasia and homogenized manually in HBSS. The lysates were filtered through a cell strainer. MLN cells were centrifuged and collected. Splenic cells were hemolyzed with 0.84% (v/w) ammonium chloride (02424-55; Nacalai Tesque), washed with HBSS, and collected for analysis.

Ono K., Sujino T., Miyamoto K., Harada Y., Kojo S., Yoshimatsu Y., Tanemoto S., Koda Y., Zheng J., Sayama K., Koide T., Teratani T., Mikami Y., Takabayashi K., Nakamoto N., Hosoe N., London M., Ogata H., Mucida D., Taniuchi I, & Kanai T. (2023). Downregulation of chemokine receptor 9 facilitates CD4+CD8αα+ intraepithelial lymphocyte development. Nature Communications, 14, 5152.

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Mdm20 Protein Antibody Generation

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Rabbit polyclonal antibody of Mdm20 was generated against the C-terminal peptide of human Mdm20 protein, which is conserved between human and mouse, fused with GST protein. The antiserum was purified using GST and protein A sepharose, columns, as described previously [20 (link)]. Anti-Nat5 (human homologue of yeast Nat3) (65145) antibody was purchased from Santa Cruz. The other antibodies used in this study were purchased as follows: anti-β-actin (A5441) antibody was purchased from Sigma; anti-Trpm (74480) and anti-phospho-PKCα (12356) antibodies were purchased from Santa Cruz; anti-phospho-Akt (Ser473 (4060), and T308 (2965)), anti-Akt (4691), anti-PKCα (2056), anti-mTOR (2983), anti-Rictor (2114), anti-Raptor (2280), anti-LST8/GβL (3274), anti-FoxO1 (2880), anti-phospho-FoxO1 (2599), anti-Cofilin (5175), and anti-phospho-Cofilin (3313) antibodies were purchased from Cell Signaling Technology; anti-LC3 (L7543) antibody was purchased from Medical and Biological Laboratories (MBL); anti-ubiquitin (MAB1510) antibody was purchased from Millipore; and anti-paxillin (610052) antibody was purchased from BD Transduction Laboratory. MG132 was purchased from Sigma, and ammonium chloride was purchased from Nacalai Tesque.

Yasuda K., Takahashi M, & Mori N. (2015). Mdm20 Modulates Actin Remodeling through the mTORC2 Pathway via Its Effect on Rictor Expression. PLoS ONE, 10(11), e0142943.

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Murine Spleen Tissue Preparation

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Spleens were harvested from the mice after death and homogenized manually in HBSS. The lysates were filtered through a cell strainer. The passed cells were hemolyzed with 0.84% (vol/wt) ammonium chloride (Nacalai Tesque), washed with HBSS, and collected for analysis.

Harada Y., Sujino T., Miyamoto K., Nomura E., Yoshimatsu Y., Tanemoto S., Umeda S., Ono K., Mikami Y., Nakamoto N., Takabayashi K., Hosoe N., Ogata H., Ikenoue T., Hirao A., Kubota Y, & Kanai T. (2022). Intracellular metabolic adaptation of intraepithelial CD4+CD8αα+ T lymphocytes. iScience, 25(4), 104021.

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Reagent Preparation for Analytical Research

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All reagents were guaranteed-grade products and obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), unless noted otherwise. 3-(Trimethoxysilyl) propyl methacryrate was obtained from Tokyo Chemical Industry Co., Ltd., (Tokyo, Japan), and 2AM18C6E was obtained from Sigma-Aldrich Co. LLC (St. Louis, USA). Sodium iodate, sodium bromate, sodium nitrate, sodium nitrite, ammonium chloride, and cesium chloride were obtained from Nacalai Tesque, Inc., (Kyoto, Japan). Calcium chloride, rubidium chloride, and lithium chloride were obtained from Yoneyama Yakuhin Kogyo Co., Ltd., (Osaka, Japan).

Tsuge K., Lim L.W, & Takeuchi T. (2021). Separation of Inorganic Anions Using an 18-Crown-6-ether-modified Organic Polymer Monolithic Stationary Phase in Capillary Ion Chromatography. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 37(6).

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Synthesis and Characterization of Zwitterionic Polymer Packing

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The reagents employed were of guaranteed grade reagents and were obtained from Wako Pure Chemical Industries (Osaka, Japan), unless otherwise noted. Sodium bromide, sodium iodate, sodium iodide, sodium nitrate, sodium nitrite, sodium thiocyanate, ammonium chloride and benzyltrimethylammonium chloride were obtained from Nacalai Tesque (Kyoto, Japan). 3-(Trimethoxysilyl)propyl methacrylate was obtained from Tokyo Chemical Industry (Tokyo, Japan). Lithium chloride, magnesium chloride hexahydrate and potassium chloride were obtained from Yoneyama Yakuhin Kogyo (Osaka, Japan).
[2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (zwitterionic monomer) was obtained from Sigma-Aldrich (St. Louis, USA). Ultrapure water was prepared in the laboratory by using a Simplicity UV water purification system (Millipore, MA, USA), and all solutions used in this study were prepared using this ultrapure water. All packing materials were packed in fused-silica capillary tube (100 × 0.32 mm i.d.; GL Sciences, Tokyo, Japan).

Takayama N., Lim L.W, & Takeuchi T. (2017). Optimization and Investigation of Zwitterionic Monolithic Stationary Phases for Capillary Ion Chromatography. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(5).

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