The method of sampling is the same as Safranin O-Fast green staining. Articular cartilage tissues were fixed in 4% paraformaldehyde and embedded in paraffin. The block was cut into 6 μm sections, and slides were prepared. Slides were incubated in primary antibodies for MMP8 (BA2201, dilution 1:100, Boster), MMP9 (ab38898, dilution 1:100, Abcam), COL1A1 (ab23446, dilution 1:100, Abcam), COL2A1 (ab34712, dilution 1:100, Abcam), and BCL2 (bs-0032R, dilution 1:200, Bioss) at 4 °C overnight. The next day, slides were washed three times and incubated with polyclonal antibodies against rabbit IgG-HRP (#SV0002, Boster) and mouse IgG-HRP (#SV0001, Boster) at room temperature for 1 h. Slides were then stained with 1 μM DAPI (Thermo Fisher Scientific, Waltham, MA, USA) to visualize cell nuclei.
Sv0001
Manufactured by Boster Bio
Sourced in United States
SV0001 is a laboratory centrifuge designed for general-purpose applications. It provides reliable performance and versatility to meet the needs of various research and testing tasks.
Automatically generated - may contain errors
Lab products found in correlation
Sv0002, by Boster Bio (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Ab23446, by Abcam (1 mentions)
Ab34712, by Abcam (1 mentions)
Bs 0032r, by Bioss Antibodies (1 mentions)
Ab38898, by Abcam (1 mentions)
Ab28364, by Abcam (1 mentions)
Ba0407, by Boster Bio (1 mentions)
3 3 diaminobenzidine (dab), by ZSGB-BIO (1 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
2 protocols using sv0001
1
Immunohistochemical Profiling of Articular Cartilage
2
Immunohistochemical Analysis of Angiogenesis
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Tissue sections (from 5 mice in each group) were incubated with 3% H2O2 for 5 min, boiled in 0.01 M citrate buffer solution (pH 6.0) for 10 min, blocked with 5% bovine serum albumin for 1 h at room temperature (RT), and incubated with primary antibodies (anti‐CD31, ab28364, 1:50; Abcam; anti‐VEGFA, BA0407, 1:200; Boster, CDK6, MA5‐13333, 1:100; Invitrogen) at 4°C overnight. The sections were then incubated with the corresponding horseradish peroxidase (HRP)‐conjugated secondary antibodies (anti‐mouse, SV0001; Boster; anti‐rabbit, SV0002; 1:10,000; Boster) at RT for 1 h, followed by DAB (ZSGB‐BIO) and hematoxylin (Guangzhou Xiuwei Company) staining. Images (3 images /section) were captured under a microscope (NIKON ECLIPSE CI). The area of positive staining was measured using Image‐Pro Plus 6.0 software (Media Cybernetics Corporation).
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