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Ab100663

Manufactured by Abcam
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Ab100663 is a laboratory equipment product. It serves as a core tool for scientific research and experimentation. The detailed technical specifications and intended use of this product are not available in this response, as per the instructions to maintain an unbiased and factual approach without extrapolation.

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Lab products found in correlation

Ab100647, by Abcam (1 mentions) Ab100637, by Abcam (1 mentions) Ab133021, by Abcam (1 mentions) Ab100549, by Abcam (1 mentions) Ab100624, by Abcam (1 mentions) Ab100540, by Abcam (1 mentions) Exl800, by Agilent Technologies (1 mentions)

4 protocols using ab100663

1

VEGF Expression in HRCEC Supernatant

Cited 1 time
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According to manufacturers’ instructions, the expression of VEGF in the supernatant of HRCECs was determined using commercial ELISA kits (Abcam; cat.: ab100663) [23 (link)].
Kong L., Li J., Yang Y., Tang H, & Zou H. (2022). Paeoniflorin alleviates the progression of retinal vein occlusion via inhibiting hypoxia inducible factor-1α/vascular endothelial growth factor/STAT3 pathway. Bioengineered, 13(5), 13622-13631.
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2

VEGF Quantification in Xenograft Phenotypes

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ELISA was performed following the manufacturer's instructions (Ab100663, Abcam). VEGF concentration (pg/μg of protein) was assessed for the three phenotypes using representative xenografts: (P8, T101 invasive; T16, P3 and NCH421k intermediate; P13, NCH644 angiogenic. Experiments were performed in triplicates with protein extract from 2 animals each. The results are presented per xenograft phenotype.
Bougnaud S., Golebiewska A., Oudin A., Keunen O., Harter P.N., Mäder L., Azuaje F., Fritah S., Stieber D., Kaoma T., Vallar L., Brons N.H., Daubon T., Miletic H., Sundstrøm T., Herold-Mende C., Mittelbronn M., Bjerkvig R, & Niclou S.P. (2016). Molecular crosstalk between tumour and brain parenchyma instructs histopathological features in glioblastoma. Oncotarget, 7(22), 31955-31971.
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3

Quantification of Inflammatory Factors

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The concentrations of PGE2 (ab133021, Abcam), SDF-1 (ab100637, Abcam), VEGF (ab100663, Abcam), IL-10 (ab100549, Abcam), and TGF-β1 (ab100647, Abcam) in culture media were measured using commercial ELISA kits according to the manufacturer's protocols.
Liu L., He Y.R., Liu S.J., Hu L., Liang L.C., Liu D.L., Liu L, & Zhu Z.Q. (2020). Enhanced Effect of IL-1β-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling. Stem Cells International, 2020, 2803747.
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4

Quantifying RPE Secreted Factors

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After 3 days of RPE cells incubation, culture media was collected and measured for PDGF-BB (Abcam; ab100624), VEFG (Abcam; ab100663) and IGFBP-2 (Abcam; ab100540) levels using human ELISA kit)according to the manufacturer's protocol. The optical densities were determined within 30 min and recorded with a microplate reader (BioTek, EXL800, Winooski, VT) at 450 nm.
Chang Y.C., Chang W.C., Hung K.H., Yang D.M., Cheng Y.H., Liao Y.W., Woung L.C., Tsai C.Y., Hsu C.C., Lin T.C., Liu J.H., Chiou S.H., Peng C.H, & Chen S.J. (2014). The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Frontiers in Aging Neuroscience, 6, 191.
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