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233 fb 001mg cf

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233-FB-001MG/CF is a laboratory product manufactured by R&D Systems. It is a recombinant human Fibroblast Growth Factor Basic Protein. The product is provided as a lyophilized powder.

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Lab products found in correlation

Cls3471, by Corning (1 mentions) Matrigel, by BD (1 mentions) 236 eg 01m, by R&D Systems (1 mentions) Neurobasal a media, by Thermo Fisher Scientific (1 mentions) Schwann cell medium, by ScienCell (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Ultra low attachment 6 well culture plates, by Thermo Fisher Scientific (1 mentions)

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233-FB/CF

3 protocols using 233 fb 001mg cf

1

Stem Cell-Derived Intestinal Organoids and Enteric Neural Crest Cells

Cited 2 times
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The University of Southern California and Children's Hospital Los Angeles Stem Cell Research Oversight committee approved all work using hPSCs. Human ES cell line H9 (WA-09), H9::SOX10::GFP, and human iPSC cell line WTC (Bruce Conklin) were maintained on Matrigel (BD Biosciences, 354234) in mTeSR (STEMCELL Technologies, 05850). HIOs were generated as described previously to day 28 to 35 of age (McCracken et al., 2011 (link), Spence et al., 2011 (link)). ENCCs were generated up to day 11 as described previously (Fattahi et al., 2016 (link)). On day 11, unsorted ENCCs were aggregated into 3D spheroids in ultra-low attachment multiwell plates (Corning Life Sciences, CLS3471) and cultured in neurobasal medium supplemented with N2/B27 containing 3 μM CHIR99021 and 10 nM FGF2 (R&D Systems, 233-FB-001MG/CF) for an additional 4 days. For in vitro long-term culture, plated ENCCs were cultured as described previously (Fattahi et al., 2016 (link)).
Schlieve C.R., Fowler K.L., Thornton M., Huang S., Hajjali I., Hou X., Grubbs B., Spence J.R, & Grikscheit T.C. (2017). Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine. Stem Cell Reports, 9(3), 883-896.
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2

In Vitro Expansion of Glioma Stem Cells

Cited 1 time
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For in vitro expansion, GSCs were cultured in Neurobasal A media (NBA; 10888-022, Gibco, Waltham, MA, USA) supplemented with N2 and B27 (0.5× each; 17502-048 and 12587-010, Gibco), human recombinant basic fibroblast growth factor, and epidermal growth factor (20 ng/mL each; 233-FB-001MG/CF and 236-EG-01M, R&D Systems, Minneapolis, MN, USA), as well as 100× penicillin streptomycin-glutamine (10378-016, Gibco) [7 (link)]. Next, GSCs were cultured at 37 °C in a humidified incubator containing 5% CO2.
Lee M., Kim Y.S., Lee K., Kang M., Shin H., Oh J.W., Koo H., Kim D., Kim Y., Kong D.S., Nam D.H, & Lee H.W. (2019). Novel Semi-Replicative Retroviral Vector Mediated Double Suicide Gene Transfer Enhances Antitumor Effects in Patient-Derived Glioblastoma Models. Cancers, 11(8), 1090.
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3

Differentiation of Neural Crest Cells into Schwann Cells

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At day 11, NC cells were aggregated into 3D spheroids (5 million cells/well) in Ultra Low Attachment 6-well culture plates (Fisher Scientific, 3471) and cultured in Neurobasal (NB) medium supplemented with L-Glutamine (Gibco, 25030–164), N2 (Stem Cell Technologies, 07156) and B27 (Life Technologies, 17504044) containing CHIR (3 μM, Tocris Bioscience, 4423) and FGF2 (10 ng/ml, R&D Systems, 233-FB-001MG/CF) and NRG1 (10 ng/ml, R&D 378-SM-025). After 14 days of suspension culture, the spheroids were plated on Poly Ornithine/Laminin/ Fibronectin (PO/LM/FN) coated dishes (prepared as described previously (17)) in Neurobasal (NB) medium supplemented with L-Glutamine (Gibco, 25030–164), N2 (Stem Cell Technologies, 07156) and B27 (Life Technologies, 17504044) containing NRG1 (20 ng/ml, R&D 378-SM-025), FGF2 (10 ng/ml, R&D Systems, 233-FB-001MG/CF) and cAMP (100 mM, Sigma, D0260). The SC precursors migrate out of the plated spheroids and differentiate into SCs within 10 days. For long-term expansion, cells were cultured in Schwann cell medium (Sciencell, 1701 without FBS) on PO/LM/FN coated dishes. Cells were fixed for immunostaining or harvested for gene expression analysis at Day 25, Day 35, Day 50 and Day 100 of differentiation.
Majd H., Amin S., Ghazizadeh Z., Cesiulis A., Arroyo E., Lankford K., Majd A., Farahvashi S., Chemel A.K., Okoye M., Scantlen M.D., Tchieu J., Calder E.L., Rouzic V.L., Shibata B., Arab A., Goodarzi H., Pasternak G., Kocsis J.D., Chen S., Studer L, & Fattahi F. (2023). Deriving Schwann Cells from hPSCs Enables Disease Modeling and Drug Discovery for Diabetic Peripheral Neuropathy. Cell stem cell, 30(5), 632-647.e10.
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