Cool snap hq2 camera

The acoustic cell setup consisted of a piezoelectric transducer
(Ferroperm PZ26 disk 10 mm diameter, 0.5 mm thickness) responsible for the
generation of ultrasound waves, attached by conductive epoxy glue to the bottom
center of a steel plate (50 mm × 50 mm × 0.94 mm³);
then the steel plate was covered with a 240 µm kapton tape protective
layer and a sample reservoir at the center (5 mm). A glass slide was used to
cover the reservoir for ultrasound reflection and to protect the sample. The
continuous ultrasound sine wave was applied via a piezoelectric
transducer, through an Agilent 15 MHz arbitrary waveform generator, in
connection to a home-made power amplifier. The applied continuous sine wave form
had a frequency of 2.56 MHz and 6 V voltage amplitude.
Videos were captured using Cool SNAP HQ² camera, 20× and 40× objectives
(unless mentioned otherwise) and acquired at the frame rate of 10 using the
Metamorph 7.1 software (Molecular Devices, Sunnyvale, CA). A Nikon Eclipse 80i
upright microscope was also used to capture time course images of the
morphological changes of AGS cells treated with US-propelled pH-responsive
polymer-CASP-3@nanomotors and other conditions.

The acoustic cell setup consisted of a piezoelectric transducer (Ferroperm PZ26 disk 10 mm diameter, 0.5 mm thickness) responsible for the generation of ultrasound waves, attached by conductive epoxy glue to the bottom center of a steel plate (50 × 50 × 0.94 mm³); then the steel plate was covered with a 240 μm Kapton tape protective layer and a sample reservoir at the center (5 mm). A glass slide was used to cover the reservoir for ultrasound reflection and to protect the sample. The continuous ultrasound sine wave was applied via a piezoelectric transducer, through an Agilent 15 MHz arbitrary waveform generator, in connection to a homemade power amplifier. All propulsion experiments were conducted by applying a continuous sine wave form that had a frequency of 2.66 MHz and an amplitude of 2.0 V, 4.0 V or 6.0 V (voltage at the output of the function generator). Videos were captured using a Cool SNAP HQ² camera, with 20× and 40× objectives and acquired at 10 frames per second using the Metamorph 7.1 software (Molecular Devices, Sunnyvale, CA). The particle displacement image stacking was performed using ImageJ software and Flow Trace Plugin.