Mice were maintained on a 12-/12-h light/dark cycle with access to standard mouse chow (Sniff) and water ad libitum. Animals were aged between 6-8 weeks old at the time of the experiment, unless otherwise stated. C57BL/6JRj female mice were purchased from Janvier Labs. Nox2 knockout mice (B6.129S-Cybbtm1Din/J; Pollock et al., 1995 (link)) were initially purchased from The Jackson Laboratory and maintained as a heterozygous breeding colonies, Nestin-GFP mice (Yamaguchi et al., 2000 (link)), were obtained from Yamaguchi and colleagues and maintained as a homozygous colony, Dcx-GFP mice (Stock Tg(Dcx-EGFP)BJ224Gsat/Mmmh) were purchased from Mutant Mouse Resource and Research center and maintained as a homozygous colony (Gong et al., 2003 (link)). Gfap-GFP mice were acquired from the lab of Frank Kirchhoff (University of Saarland) and maintained as a homozygous colony (Nolte et al., 2001 (link)). Mice were housed in groups of at least two mice in standard polycarbonate cages (Type III, Techniplast, Germany), except for the animals with running wheels, which were singly-housed. All experiments were conducted in accordance with the applicable European regulations and approved by the responsible authority (Landesdirektion Sachsen). Detailed information on the genotype of the animals used for each experiment; the number of animals per sample and sample size is given in Table S5 .
Female c57bl 6jrj mice
Manufactured by Janvier Labs
Sourced in France
Female C57BL/6JRj mice are a commonly used inbred strain of laboratory mice. These mice are a well-established model for biomedical research.
Automatically generated - may contain errors
Lab products found in correlation
B6.129s cybbtm1din j, by Jackson ImmunoResearch (1 mentions)
Balb cannrj foxn1nu foxn1nu mice, by Janvier Labs (1 mentions)
Balb cann cg foxn1nu crl mice, by Charles River Laboratories (1 mentions)
Dsred, by Takara Bio (1 mentions)
Male wistar rats, by Janvier Labs (1 mentions)
Rompun, by Bayer (1 mentions)
Type 2, by Tecniplast (1 mentions)
14 protocols using female c57bl 6jrj mice
1
Mouse Models for Neuroscience Research
2
Murine Oral Cancer Cell Inoculation
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Two Mouse Oral Cancer (MOC) cell lines were obtained (Kerafast, USA). MOC1 is a cell line with indolent growth in vivo and it is very immunogenic, while MOC2 is a less immunogenic, aggressive cancer with rapid growth in vivo and propensity to metastases28 (link),29 (link). C57BL/6Jrj female mice were used throughout (Janvier, France) after a minimum of 2 weeks of acclimatization in the animal facility at Danish Centre for Particle therapy, Aarhus, Denmark, where they were stalled in cages of 4 mice. The mice were around 9–18 weeks at inoculation (Supplementary Tables S1 and S2 ). Tumor tissue was first implanted into the mouse flank. When the tumor reached a size of approximately 1000 mm3 it was harvested and processed into a cell suspension in PBS, and 5–8 µl was injected subcutaneously into the right hind leg of each mouse. All animal experiments were performed in accordance with the animal welfare policy of Aarhus University and approved by the Danish Animal Experiments Inspectorate.
3
Animal Models for Imaging and Tracking
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Experiments
were performed
in accordance with the guidelines set for animal care of the Nijmegen
and European Animal Experiments Committee, and the animals were maintained
in individually ventilated cages blueline. Nine C57BL/6JRj female
mice, age 6–8 weeks and an average weight of 20 ± 0.9
g, were obtained from Janvier Laboratories for biodistribution and
blood clearance of [111In]In-PLGA-NH2 NPs (CCD
application 2015-0071). BALB/cAnNRj-Foxn1nu/Foxn1nu mice (12 mice,
female, Janvier Laboratories) at age 6–8 weeks and average
weight of 23.24 ± 1.36 g were used for the in vivo SPECT/CT imaging
of [111In]In-THP-1 cells in Matrigel (CCD application 2018-0011).
BALB/CAnN.Cg-Foxn1nu/Crl mice (6 mice, female, Charles River) at age
6–8 weeks and average weight of 19.1 ± 1.01 g were used
for the Staphylococcus aureus (S. aureus) model for in vivo tracking of [111In]In-THP-1 cells
experiments (CCD application 2020-0007).
were performed
in accordance with the guidelines set for animal care of the Nijmegen
and European Animal Experiments Committee, and the animals were maintained
in individually ventilated cages blueline. Nine C57BL/6JRj female
mice, age 6–8 weeks and an average weight of 20 ± 0.9
g, were obtained from Janvier Laboratories for biodistribution and
blood clearance of [111In]In-PLGA-NH2 NPs (CCD
application 2015-0071). BALB/cAnNRj-Foxn1nu/Foxn1nu mice (12 mice,
female, Janvier Laboratories) at age 6–8 weeks and average
weight of 23.24 ± 1.36 g were used for the in vivo SPECT/CT imaging
of [111In]In-THP-1 cells in Matrigel (CCD application 2018-0011).
BALB/CAnN.Cg-Foxn1nu/Crl mice (6 mice, female, Charles River) at age
6–8 weeks and average weight of 19.1 ± 1.01 g were used
for the Staphylococcus aureus (S. aureus) model for in vivo tracking of [111In]In-THP-1 cells
experiments (CCD application 2020-0007).
4
Standardized Murine Experimentation Protocol
5
In vivo Trypanosoma brucei brucei Protocol
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Female C57BL/6JRj mice (Mus musculus, 6–8 weeks) were purchased from Janvier (Le Genest-Saint-Isle, France) and were used for all in vivo experiments. Food for laboratory rodents (Carfil, Arendonk, Belgium) and drinking water were available ad libitum. Animals were housed in an individually ventilated caging system controlled at 22–26 °C, 40–60% relative humidity and a 12 h alternating dark/light cycle. All housing standards specified in Annex 4 of the Belgian Royal Decree of 29 May 2013 were respected. The animals were kept in quarantine for at least 5 days before infection and were randomly allocated to the experimental units.
Trypanosoma brucei brucei AnTAR1 and two transgenic strains of the pleomorphic T. b. brucei AnTat1.1E expressing the red fluorescent protein of a Discosoma coral (DsRED, Clontech)11 (link) or a red-shifted firefly luciferase (PpyRE9)23 (link) were used for flow cytometry and bioluminescent imaging, respectively [kind gifts of Nick Van Reet and Philippe Büscher (Institute of Tropical Medicine, Antwerp, Belgium)].
Trypanosoma brucei brucei AnTAR1 and two transgenic strains of the pleomorphic T. b. brucei AnTat1.1E expressing the red fluorescent protein of a Discosoma coral (DsRED, Clontech)11 (link) or a red-shifted firefly luciferase (PpyRE9)23 (link) were used for flow cytometry and bioluminescent imaging, respectively [kind gifts of Nick Van Reet and Philippe Büscher (Institute of Tropical Medicine, Antwerp, Belgium)].
6
Female C57Bl/6J Mice Acclimatization
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Female C57Bl/6Jrj mice (8 weeks old) were obtained from Janvier Labs (Saint Berthevin Cedex, France) and allowed to acclimatize prior to procedures in groups of 4 for 1 week in individually ventilated cages with a 12‐h light cycle with ad libitum access to standard chow and water. Animals were randomly assigned to the different equally sized treatment groups and the investigators were blinded regarding treatment. Animal studies are reported in compliance with the ARRIVE guidelines (Percie du Sert et al., 2020 (link)) and with the recommendations made by the British Journal of Pharmacology (Lilley et al., 2020 (link)). Animal experiments were performed with permission from the Danish Animal Experiments Inspectorate (licence 2013‐15‐2934‐00833) and the local ethical committee following the guidelines of Danish legislation governing animal experimentation (1987) and the National Institutes of Health (publication number 85‐23). All efforts were made to diminish animal sufferings and animal numbers used.
7
Transgenic Mouse Models for Metabolic Research
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All animal experiments were performed in accordance with the Danish Animal Experiments Inspectorate, Ministry of Environment and Food of Denmark, permits 2013-15-2934-00833 and 2018-15-0201-01397, the EU directive 2010/63/EU, and the National Institutes of Health (publication No. 85-23). All studies were approved by the local ethical committee.
Female C57BL/6JRj mice (10–12 weeks of age), male ob/ob (10 weeks of age), and male Wistar rats (250 g) were obtained from Janvier Labs (Saint-Berthevin, France). Transgenic mice (TgN(GCG.DTR)) were previously described [22 (link)]. Male Zucker lean and fatty rats were obtained from Charles River (Italy). Female TgN(GCG.DTR), male Gcgr−/−, and wild-type littermates of both mouse strains were obtained from the local breeding facility (7–12 weeks of age). Mice were housed in groups of maximum eight and rats in groups of maximum four in individually ventilated cages. All animals followed a 12-h light cycle (lights on 6 AM to 6 PM) with ad libitum access to chow (catalog no. 1319, Altromin Spezialfutter, Lage, Germany) and water and were allowed to acclimatize for at least one week after arrival before use. During all studies with transgenic animals, the investigator was blinded to the genotype to avoid bias. On the experimental day, the mice were fasted from 8 AM to 12 PM with free access to water.
Female C57BL/6JRj mice (10–12 weeks of age), male ob/ob (10 weeks of age), and male Wistar rats (250 g) were obtained from Janvier Labs (Saint-Berthevin, France). Transgenic mice (TgN(GCG.DTR)) were previously described [22 (link)]. Male Zucker lean and fatty rats were obtained from Charles River (Italy). Female TgN(GCG.DTR), male Gcgr−/−, and wild-type littermates of both mouse strains were obtained from the local breeding facility (7–12 weeks of age). Mice were housed in groups of maximum eight and rats in groups of maximum four in individually ventilated cages. All animals followed a 12-h light cycle (lights on 6 AM to 6 PM) with ad libitum access to chow (catalog no. 1319, Altromin Spezialfutter, Lage, Germany) and water and were allowed to acclimatize for at least one week after arrival before use. During all studies with transgenic animals, the investigator was blinded to the genotype to avoid bias. On the experimental day, the mice were fasted from 8 AM to 12 PM with free access to water.
8
Mouse Model of Ovalbumin-Induced Asthma
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Female C57BL/6JRj mice (8 weeks old, Janvier, France) were treated with particle suspensions or control solutions via pharyngeal aspiration with a volume of 50 μL, under inhalation anaesthesia (isoflurane, 5% in synthetic air, 1-2 min). Animals were sensitized by repetitive intraperitoneal injection of 1 μg OVA/alum. At the indicated time point, mice were challenged by aerosol inhalation (1% OVA in PBS) for 30 min using a Pari-Boy Nebuliser (Pari, Starnberg, Germany). Animals were sacrificed by exsanguination under anaesthesia after the indicated exposure times. Serum was prepared from blood samples taken prior to exsanguination. Bronchoalveolar lavage was taken using 4 × 1 mL PBS. All animal experiments were performed after relevant permission according to German animal protection laws.
9
Particle Administration in Mice
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Female C57BL/6JRj mice were purchased from Janvier Labs (St Bertevin, France). Eight-week-old animals were kept with sterile rodent feed and acidified water, and housed in positive-pressure air-conditioned units (25 °C, 50% relative humidity) on a 12 h light/dark cycle. For aspiration, mice were anaesthesized with a mix of Nimatek, 1 mg/mouse (Eurovet, Bladel, Netherlands) and Rompun, 0.2 mg/mouse (Bayer, Kiel, Germany) given intraperitoneally, and administered a 50 μl suspension of particles or suspension medium at pH 7.5 (controls) by oro-pharyngeal aspiration. For gavage, mice were administered 200 μL suspension or dispersion medium. Mice were sacrificed 3 d after particle administration with an intraperitoneal injection of 12 mg sodium pentobarbital (Certa, Braine-l’Alleud, Belgium).
10
Topical Imiquimod and Sanguinarine Protocol
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Female C57BL6/JRj mice (aged 8‒10 weeks) were purchased from Janvier Labs (Saint Berthevin, France). All experiments were carried out in strict accordance with the rules of the French Ministry of Agriculture and the European Communities Council Directive (86/609/EEC). Experimental procedures were approved by a local ethical committee (Comité d'Ethique pour l'Expérimentation Animale, Campus d'Orléans [French Registration CECCO 03]). Mice were shaved on the back skin, randomly divided into three groups of seven mice each, and then topically treated with either 200 μl acetone alone (control group) or 62.5 mg IMQ from a commercially available cream (Aldara 5%, 3M Pharmaceuticals, Saint Paul, MN) (IMQ group) or 9 mg SM in 200 μl acetone for 45 minutes before the IMQ treatment (IMQ + SM) once daily for 6 consecutive days. On the seventh day, all the mice were killed, and their skin was collected for further analysis.
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