Phenyl hexyl column

Manufactured by Phenomenex

Sourced in United States

The Phenyl-Hexyl column is a reversed-phase liquid chromatography column designed for the separation and analysis of a wide range of organic compounds. The stationary phase consists of silica particles modified with phenyl-hexyl functional groups, providing a unique selectivity for the separation of analytes. This column is suitable for a variety of applications, including the analysis of pharmaceuticals, environmental samples, and other complex mixtures.

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Lab products found in correlation

Api 5500, by AB Sciex (1 mentions) Api 5000, by AB Sciex (1 mentions) Luna c18 column, by Phenomenex (1 mentions) Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions) Unity inova spectrometer, by Agilent Technologies (1 mentions) Xevo g2 qtof ms spectrometer, by Waters Corporation (1 mentions) Chirascan plus, by Applied Photophysics (1 mentions) Avance spectrometer, by Bruker (1 mentions) P 2000 polarimeter, by Jasco (1 mentions) Acquity uplc h class, by Waters Corporation (1 mentions) Empower 2, by Waters Corporation (1 mentions) Agilent 1260, by Agilent Technologies (1 mentions) 1260 infinity, by Agilent Technologies (1 mentions) Agilent 1200 series hplc, by Agilent Technologies (1 mentions) Agilent 1290 lc, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Luna Phenyl-Hexyl column (24 citations) Kinetex Phenyl-Hexyl column (11 citations) Phenyl-Hexyl (10 citations) Luna Phenyl-Hexyl analytical column (3 citations) Luna 5 μm Phenyl Hexyl column (3 citations) Luna-5μ-Phenyl-Hexyl column (3 citations) Luna Phenyl-Hexyl 100 Å column (2 citations) PhenomenexLuna phenyl-hexyl column (2 citations) Kinetex Phenyl-Hexyl C18 column (2 citations) Kinetex phenyl-hexyl 100 A column (2 citations)

7 protocols using phenyl hexyl column

Phenolic Benzotriazoles Quantification in Plasma

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Chromatographic parameters were developed and optimized for the analysis of the nine phenolic benzotriazoles and tBuPrOH-BZT. Samples were analyzed using a Shimadzu Prominence (Kyoto, Japan) LC coupled to AB Sciex (Framingham, MA) API 5000 or API 5500 MS in a positive chemical ionization mode. Phenomenex Kinetex, Phenyl-Hexyl column (50 m × 2.1 mm (ID) × 2.6 μm film thickness) (Torrance, CA) and guard column Phenomenex Security Guard (C18, 4×2 mm) were used with a flow rate of 300 μL/min. The method details are provided in Table S2.
A linear regression with 1/x weighting was used for free P-BZT, free and total octrizole, free tBuPrOcEst-BZT, total tBuPrOH-BZT, free and total bumetrizole, free DiMeEtPh-BZT, free and total ditBuCl-BZT, free and total DitPe-BZT, and total tBu-BZT to relate analyte to IS peak area response ratio to analyte concentration in plasma. A linear regression with 1/x² was used for total P-BZT, free and total drometrizole, total DiMeEtPh-BZT, and free tBu-BZT to relate peak area response ratio to analyte concentration in plasma. Plasma concentration of each free and total phenolic benzotriazoles were reported as ng analyte/mL plasma.

Mutlu E., South N., Pierfelice J., Djonabaye A., Pauff M., Burback B, & Waidyanatha S. (2022). Quantitation of Phenolic Benzotriazole Class Compounds in Plasma by Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS). Analytical letters, 55(13), 2074-2088.

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Purification of Compound 7 from Nonomuraea

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A total of 4 L of Nonomuraea sp. ATCC 55076 were grown in GFM medium at 250 rpm and 30 °C for 9 days, as described above. Cells were removed by centrifugation and the supernatant extracted twice with acidified ethyl acetate (supplemented with 0.1% formic acid). The extract was dried over Na₂SO₄, then dried completely in vacuo. The residue was resuspended in MeOH and resolved on a preparative Agilent HPLC on a Phenomenex Luna C18 column (5 μm, 21.2 × 250 mm) at a flow rate of 12 mL min^–1. The solvent system consisted of water and MeCN, each containing 0.1% formic acid. A gradient from 20% to 100% MeCN over 40 min was used to purify compound 7, which eluted at 65% MeCN. Compound 7 was purified further on an Agilent Eclipse C8 column (5 μm, 4.6 × 150 mm), a Supelco C18-PEG column (4 μm, 4.6 × 250 mm), and finally a Phenomenex Kinetex Phenyl-hexyl column (5 μm, 4.6 × 100 mm). Elution on each of these columns was performed isocratically at 40% MeCN at 1 mL min^–1 by monitoring the signal at 280 nm. Pure 7 was characterized by NMR (Fig. S3 and Table S2‡) and HR-MS, which gave [M + H]+obs 213.0654 ([M + H]+calc 213.0664).

Nazari B., Forneris C.C., Gibson M.I., Moon K., Schramma K.R, & Seyedsayamdost M.R. (2017). Nonomuraea sp. ATCC 55076 harbours the largest actinomycete chromosome to date and the kistamicin biosynthetic gene cluster †The authors declare no competing interests. ‡Electronic supplementary information (ESI) available. See DOI: 10.1039/c6md00637j. MedChemComm, 8(4), 780-788.

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Analytical Methods for Natural Product Isolation

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Optical rotations were recorded on a JASCO P-2000 polarimeter (JASCO International Co., Ltd., Tokyo, Japan). IR data were collected using a Nicolet 6700 FT-IR spectrometer (Thermo Electron Corp., Waltham, MA, USA). ECD spectra were obtained using Chirascan Plus (Applied Photophysics Ltd., Surrey, United Kingdom). A Waters Xevo G2 QTOF MS spectrometer (Waters Co., Milford, MA, USA) was used for high-resolution electrospray ionization mass spectrometry (HRESIMS) values. Semipreparative HPLC experiments were performed using a Gilson HPLC system with a 321 pump and a UV/VIS-155 detector. A Phenomenex Phenyl-Hexyl column (10 × 250 mm, 5 μm particle size, USA) was used as the HPLC column. The NMR spectra for 1D (¹H and ¹³C) and 2D (HSQC, HMBC, NOESY, and ROESY) were measured on a Varian Unity Inova spectrometer at 500 MHz (Agilent Technologies, Santa Clara, CA) and an AVANCE spectrometer at 800 MHz (Bruker, Germany). YMC*Gel ODS-A and Sephadex LH-20 were used for column chromatography. Thin-layer chromatography experiments were performed with silica gel 60 F₂₅₄ and RP-18 F₂₅₄ plates. A Gilson semi-Prep HPLC system was composed of a flow rate of 3 mL/min and UV detection at 201 and 254 nm. All solvents (Dae Jung Pure Chemical, Siheung, Korea) for extraction and isolation were of analytical grade.

Lee H.J., Park E.J., Ryu B., Cho H.M., Nghiem D.T., Pham H.T., Pan C.H, & Oh W.K. (2022). Benzylisoquinoline Alkaloids from the Stems of Limacia scandens and Their Potential as Autophagy Inhibitors. Pharmaceuticals, 15(11), 1332.

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Quantification of Glutathione and Cysteine

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Glutathione and cysteine (Cys) were determined by derivatization with p-benzoquinone (pBQ) [29 (link)]. Briefly, MW and WW samples (2 mL) were derivatized with pBQ (100 μL, 8 mM) followed by the addition of 3MPA (1 mL, 1.5 M). The reaction mix was filtered through a 0.22 μm pore-size PVDF membrane (Millipore, Billerica, MA, USA) and analyzed by an Acquity HClass UPLC (Waters) system equipped with a photo diode array detector 2996 (Waters) using a phenyl-hexyl column (250 × 4.6 mm, 5 μm, 110 Å, Phenomenex, Torrance, CA, USA). The separation was carried out with (solvent A) water/trifluoroacetic acid 0.05% (v/v) and (solvent B) methanol in gradient mode (from 10% B to 35% B in 18 min) at a flow rate of 1 mL/min [29 (link)]. The detection wavelength was 303 nm; data acquisition and processing were performed by Empower 2 software (Waters).

Fracassetti D., Limbo S., Messina N., Pellegrino L, & Tirelli A. (2021). Light-Struck Taste in White Wine: Protective Role of Glutathione, Sulfur Dioxide and Hydrolysable Tannins. Molecules, 26(17), 5297.

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HPLC Analysis of Furanocoumarins

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Analysis of EBGT markers was carried out according to a previously validated method [2 (link)]. Briefly, chromatographic analysis was performed using an HPLC Agilent 1260 system (Agilent Technologies, Santa Clara, CA, USA) equipped with a photodiode array (DAD) and a fluorescence detector (FLD). The mobile phase consisted of acetonitrile as solvent A and 0.04% trifluoroacetic acid as solvent B. PSO and 5-MOP were assayed by the HPLC-DAD (Infinity 1260, Agilent Technologies) on a Phenyl-hexyl column (150 × 4.6 mm, 5 µm, Phenomenex) using 40% A for 7.0 min at a flow rate of 1.0 mL min⁻¹ and detection at 245 nm. Sample injection volume was 25 µL.

Machado R.D., Silva J.C., Silva L.A., Oliveira G.D., Lião L.M., Lima E.M., de Morais M.C., da Conceição E.C, & Rezende K.R. (2022). Improvement in Solubility–Permeability Interplay of Psoralens from Brosimum gaudichaudii Plant Extract upon Complexation with Hydroxypropyl-β-cyclodextrin. Molecules, 27(14), 4580.

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Quantification of Fimasartan in Human Plasma by LC-MS/MS

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The quantification of fimasartan in human plasma was carried out with LC -MS/MS system using an Agilent 1200 series HPLC (Agilent Technologies, USA) coupled with an Applied Biosystems API3200 triple quadrupole mass spectrometer (Applied Biosystems, USA), equipped with an electrospray ionization source operated in negative ionization mode. The analytical column used was a Phenyl-Hexyl column (Luna w , 5 mm, 50 mmÂ2.0 mm; Phenomenex, USA) at a temperature of 258C. The samples were separated under the gradient condition using mobile phase A (double-distilled water with 0.1% formic acid) and mobile phase B (acetonitrile with 0.1% formic acid). The gradient condition used is described in Table I. The flow rate was maintained at 0.25 mL/min. The ion spray voltage was set at 4,000 V and at a temperature of 6008C. Voltages within the ion source were as follows: collision energy, entrance potential, collision cell exit potential and declustering potential of 246, 24, 24 and 250 V and 252, 24, 24 and 260 V for fimasartan and IS, respectively.

Yoon S.H., Oh S., Kim H.S., Yi S., Yu K.S., Jang I.J, & Cho J.Y. (2015). Validated LC-MS/MS Assay for the Quantitative Determination of Fimasartan in Human Plasma: Application to Pharmacokinetic Studies. Journal of chromatographic science, 53(8).

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TCIPP Kinetics Analysis in HLM and Serum

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For the TCIPP kinetics studies in HLM and serum, extracts were analyzed on an Agilent 1290 LC coupled to a 6460 triple quadrupole MS. Phenylhexyl column (100 x 2.1 x 2.6 µm, Phenomenex) was used for separation of the extracts. The mobile phase consisted of 5 mM ammonium acetate in water (A) and acetonitrile (B), with the following gradient conditions for HLM extracts: 10% B (0.5 min hold), increase to 30% B in 2.5 min (2 min hold), increase to 40% B in 5 min, followed by a sharp increase to 95% B (3.5 min hold), and equilibration at starting conditions for 3.5 min. A different gradient program was used for serum extracts: 5% B (0-2 min), 20% B (at 2.5 min), 30% B (4 min), 40% B (6 min), 95% B (11-11.5 min), and equilibration at starting conditions for 7 min.
Flow rate was 0.3 mL/min, temperature 40 °C, injection volume 1 µL. The following MS parameters were used: gas temperature 325 °C, sheath gas heater 250 °C, gas flow 10 L/min, sheath gas flow 11 L/min, nebulizer pressure 35 psi, capillary and nozzle voltage 3500 and 0 V, respectively. MRM transitions can be found in Table A1.

Van den Eede N., Tomy G., Tao F., Halldorson T., Harrad S., Neels H, & Covaci A. (2016). Kinetics of tris (1-chloro-2-propyl) phosphate (TCIPP) metabolism in human liver microsomes and serum. Chemosphere, 144.

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