Mcf10a cdh1
The MCF10A-CDH1−/− is a human breast epithelial cell line that has a homozygous knockout of the CDH1 gene, which encodes the E-cadherin protein. This cell line is commonly used in cell biology research to study the role of E-cadherin in cellular processes such as cell-cell adhesion and epithelial-mesenchymal transition.
Lab products found in correlation
4 protocols using mcf10a cdh1
Cell Line Maintenance and Validation Protocol
Isogenic MCF10A and NCI-N87 Cell Lines
Isogenic Cell Line Generation and Culture
Isogenic MCF10A Cell Line Comparison
Vorinostat was purchased from SelleckChem (Houston, TX), and paclitaxel (Taxol) was purchased from Sigma-Aldrich. Both drugs were reconstituted in 100% DMSO, stored at -80 °C, and individual aliquots were diluted to working stocks in complete growth medium prior to use in an experiment. Vorinostat, a histone deacetylase inhibitor, was selected because it has previously demonstrated selective lethality toward CDH1-deficient MCF10A cells. 9 Taxol was chosen as a chemotherapeutic agent that demonstrated nondiscriminate lethality in the MCF10A isogenic cell line pair.
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