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Z ll 2 ketone

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(Z-LL)2 ketone is a laboratory compound that functions as a proteasome inhibitor. It is used in research applications to study cellular processes and protein degradation pathways.

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Lab products found in correlation

Calpain activation inhibitor e64d, by Santa Cruz Biotechnology (2 mentions) L 685 458, by Merck Group (2 mentions) Recombinant ifn γ, by Thermo Fisher Scientific (1 mentions) Mouse anti human cd14 pacific blue, by BioLegend (1 mentions) Goat anti human β actin, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse af647, by Thermo Fisher Scientific (1 mentions) Mouse anti human lamp1, by BD (1 mentions)

3 protocols using z ll 2 ketone

1

Modulation of SPPL2a Deficiency

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Freshly isolated PBMCs or EBV-B cells (106 cells/ml) in RPMI supplemented with 10% FCS were incubated with or without 1 μM DMSO, 1 μM gamma secretase/SPPL inhibitor L-685,458 (EMD Millipore), 10 μM (Z-LL)2 ketone (Santa Cruz), 10 μM calpain activation inhibitor E64d (Santa Cruz) or 10 μM DAPT (another gamma secretase inhibitor, from R&D Systems) for 5 h for cytokine production and for 24 hours to study CD74 accumulation at 37°C, to mimic the conditions of SPPL2a deficiency.
Kong X.F., Martinez-Barricarte R., Kennedy J., Mele F., Lazarov T., Deenick E.K., Ma C.S., Breton G., Lucero K.B., Langlais D., Bousfiha A., Aytekin C., Markle J., Trouillet C., Jabot-Hanin F., Lindestam Arlehamn C.S., Rao G., Picard C., Lasseau T., Latorre D., Hambleton S., Deswarte C., Itan Y., Abarca K., Moraes-Vasconcelos D., Ailal F., Ikinciogullari A., Dogu F., Benhsaien I., Sette A., Abel L., Boisson-Dupuis S., Schröder B., Nussenzweig M.C., Liu K., Geissmann F., Tangye S.G., Gros P., Sallusto F., Bustamante J, & Casanova J.L. (2018). Disruption of an anti-mycobacterial circuit between dendritic and Th cells in human SPPL2a deficiency. Nature immunology, 19(9), 973-985.
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2

Modulation of SPPL2a Deficiency

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Freshly isolated PBMCs or EBV-B cells (106 cells/ml) in RPMI supplemented with 10% FCS were incubated with or without 1 μM DMSO, 1 μM gamma secretase/SPPL inhibitor L-685,458 (EMD Millipore), 10 μM (Z-LL)2 ketone (Santa Cruz), 10 μM calpain activation inhibitor E64d (Santa Cruz) or 10 μM DAPT (another gamma secretase inhibitor, from R&D Systems) for 5 h for cytokine production and for 24 hours to study CD74 accumulation at 37°C, to mimic the conditions of SPPL2a deficiency.
Kong X.F., Martinez-Barricarte R., Kennedy J., Mele F., Lazarov T., Deenick E.K., Ma C.S., Breton G., Lucero K.B., Langlais D., Bousfiha A., Aytekin C., Markle J., Trouillet C., Jabot-Hanin F., Lindestam Arlehamn C.S., Rao G., Picard C., Lasseau T., Latorre D., Hambleton S., Deswarte C., Itan Y., Abarca K., Moraes-Vasconcelos D., Ailal F., Ikinciogullari A., Dogu F., Benhsaien I., Sette A., Abel L., Boisson-Dupuis S., Schröder B., Nussenzweig M.C., Liu K., Geissmann F., Tangye S.G., Gros P., Sallusto F., Bustamante J, & Casanova J.L. (2018). Disruption of an anti-mycobacterial circuit between dendritic and Th cells in human SPPL2a deficiency. Nature immunology, 19(9), 973-985.
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3

Inhibition of Lysosomal Protease Activity

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(Z‐Leu‐Leu‐NHCH2)2CO, 1,3‐di‐(N‐carboxybenzoyl‐ l‐leucyl‐ l‐leucyl)amino acetone, and 2,2′‐(2‐oxo‐1,3‐propanediyl)bis[N‐[(phenylmethoxy)carbonyl]‐ l‐leucyl‐ l‐leucinamide) ((Z‐LL2)‐ketone) were purchased from Santa Cruz (sc‐311559); N‐morpholinurea–leucine–homophenylalanine–vinylsulfone‐phenol (LHVS) was synthesized in the laboratory of Dr. C Driessen, Department of Oncology and Hematology, Cantonal Hospital St Gallen, St Gallen, Switzerland, following protocols as published in [27]. Recombinant IFN‐γ was purchased from eBioscience (34‐8319‐82). The following antibodies were used: mouse anti‐human LAMP‐1 PE (BD; clone H4A3; 555801), mouse anti‐human CLIP FITC (BD; clone CerCLIP; 555981) mouse anti‐human CD74 PE (Biolegend; clone LN2; 326807), mouse anti‐human CD14 Pacific blue (Biolegend; clone M5E2; 301815), mouse anti‐human HLA ABC PE‐Cy7 (BD; clone G46‐2.6; 561349), mouse anti‐human HLA DR PerCP (Biolegend; clone L243; 560896), mouse anti‐human CD74 N‐terminal (Abcam; clone 2D1B3; ab181465), goat anti‐human β actin (Santa Cruz; clone I‐19; SC1616), goat anti‐mouse AF647 (Thermofisher Scientific; A28181), rabbit anti‐human Na‐K ATPase (Cell Signalling; 3010s), mouse anti‐human LAMP1 (BD, clone H4A3; 555798), Rabbit Anti‐mouse‐HRP antibody, (Dako; P0161), and Rabbit anti‐goat HRP antibody (Dako; P0160).
van Kempen T.S., Leijten E.F., Lindenbergh M.F., Nordkamp M.O., Driessen C., Lebbink R., Baerlecken N., Witte T., Radstake T.R, & Boes M. (2020). Impaired proteolysis by SPPL2a causes CD74 fragment accumulation that can be recognized by anti‐CD74 autoantibodies in human ankylosing spondylitis. European Journal of Immunology, 50(8), 1209-1219.
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