Log In Sign up
The largest database of trusted experimental protocols

Ccr7 percp cy5.5 clone g043h7

Manufactured by BioLegend
Visit Supplier

CCR7-PerCP-Cy5.5 (clone G043H7) is a fluorescently-labeled antibody that binds to the CCR7 cell surface receptor. CCR7 is a chemokine receptor that plays a role in lymphocyte migration and homing. The PerCP-Cy5.5 fluorophore is used for detection by flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

7 aad, by Thermo Fisher Scientific (1 mentions) Aqua live dead stain, by Thermo Fisher Scientific (1 mentions) Cd8 pb clone rpa t8, by BD (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Cd45ra bv605 clone hi100, by BioLegend (1 mentions) Granzyme b fitc clone gb11, by BioLegend (1 mentions) Intracellular fixation permeabilization buffer set, by Thermo Fisher Scientific (1 mentions) Cd3 bv785 clone okt3, by BioLegend (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

Close spelling variants of this product

CCR7 (clone G043H7, CCR7-PerCP Cy5.5 CCR7 (G043H7, Clone G043H7 PerCP-Cy5.5

4 protocols using ccr7 percp cy5.5 clone g043h7

1

Phenotypic Characterization of Plasmid-induced Activation in pDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enriched human pDCs were transferred in U-bottom 96-well plates (5.0 x 104 cells/well) and incubated at 37°C in 5% CO2 with ICs generated by mixing 0.5 μM of CG50 plasmid DNA with IgGD, IgED or a combination of both. Surface marker expression was assessed by flow cytometry using CD83-PE-CF594 (clone HB15e), CD86-AF700 (clone FUN-1) from BD Biosciences and CCR7-PerCP-Cy5.5 (clone G043H7), from Biolegend.
Henault J., Riggs J.M., Karnell J.L., Liarski V.M., Li J., Shirinian L., Xu L., Casey K.A., Smith M.A., Khatry D.B., Izhak L., Clarke L., Herbst R., Ettinger R., Petri M., Clark M.R., Mustelin T., Kolbeck R, & Sanjuan M.A. (2015). Self-reactive IgE exacerbates interferon responses associated with autoimmunity. Nature immunology, 17(2), 196-203.
+ Open protocol
+ Expand
2

Phenotypic Characterization of Plasmid-induced Activation in pDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enriched human pDCs were transferred in U-bottom 96-well plates (5.0 x 104 cells/well) and incubated at 37°C in 5% CO2 with ICs generated by mixing 0.5 μM of CG50 plasmid DNA with IgGD, IgED or a combination of both. Surface marker expression was assessed by flow cytometry using CD83-PE-CF594 (clone HB15e), CD86-AF700 (clone FUN-1) from BD Biosciences and CCR7-PerCP-Cy5.5 (clone G043H7), from Biolegend.
Henault J., Riggs J.M., Karnell J.L., Liarski V.M., Li J., Shirinian L., Xu L., Casey K.A., Smith M.A., Khatry D.B., Izhak L., Clarke L., Herbst R., Ettinger R., Petri M., Clark M.R., Mustelin T., Kolbeck R, & Sanjuan M.A. (2015). Self-reactive IgE exacerbates interferon responses associated with autoimmunity. Nature immunology, 17(2), 196-203.
+ Open protocol
+ Expand
3

Multiparametric Profiling of Cryopreserved Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved TIL or PBMCs were thawed, washed in FACS (Fluorescence Activated Cell Sorting) Wash Buffer [1X Dulbecco’s phosphate buffered saline (PBS) with 1% Bovine Serum Albumin]and stained for cell surface markers; CD8 PB (clone RPA-T8, BD (BD Biosciences)), MART-1 dextramer APC (Immudex), CCR7 PerCP-Cy5.5 (clone G043H7 BioLegend), CD45RA FITC (clone HI100, BD), CD27 APC-H7 (clone M-T271, BD), CD28 PE-Cy7 (clone CD28.2, BD), CD3 PE (clone SK7, BD). A viability stain was included for dead cell exclusion (the fixable dye Live/dead stain-Aqua, Invitrogen, or 7-AAD (7-Aminoactinomycin D) for fresh stains). The cells were fixed and acquired using a BD FACS Canto II flow cytometer. Live cells were gated based on fluorescence minus one controls. The data were analyzed using Flow Jo software (BD).
Saberian C., Amaria R.N., Najjar A.M., Radvanyi L.G., Haymaker C.L., Forget M.A., Bassett R.L., Faria S.C., Glitza I.C., Alvarez E., Parshottam S., Prieto V., Lizée G., Wong M.K., McQuade J.L., Diab A., Yee C., Tawbi H.A., Patel S., Shpall E.J., Davies M.A., Hwu P, & Bernatchez C. (2021). Randomized phase II trial of lymphodepletion plus adoptive cell transfer of tumor-infiltrating lymphocytes, with or without dendritic cell vaccination, in patients with metastatic melanoma. Journal for Immunotherapy of Cancer, 9(5), e002449.
+ Open protocol
+ Expand
4

Comprehensive SARS-CoV-2 PBMC Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
After stimulation with SARS-CoV-2 OPP, PBMCs were stained with fluorophore conjugated antibodies for flow cytometry analysis. Mastermixes, containing all antibodies for intra or extracellular staining in the optimal concentrations were prepared directly before staining. At first, cells were surface stained with CCR7-PerCP-Cy5.5 (clone G043H7) (BioLegend), CD4-A700 (clone OKT4) (BioLegend), Fixable Viability Dye eFluor780 (eBioscience), CD8-V500 (clone RPA-T8) (BD Biosciences) and CD45RA-BV605 (clone HI100) (BioLegend) for 10 minutes at room temperature in the dark. After thoroughly washing with PBS/BSA, cells were fixed and permeabilized using Intracellular Fixation & Permeabilization Buffer Set according to the manufacturer’s instructions (Thermo Fisher Scientific). Then, the cells were stained with Granzyme B-FITC (clone GB11) (BioLegend), IL2-PE (clone MQ1-17H12) (BioLegend), IL4-PE-Dazzle594 (clone MP4-25D2) (BioLegend), CD137 (4-1BB)-PE-Cy7 (clone 4B4-1) (BioLegend), CD154 (CD40L)-A647; (clone 24-31) (BioLegend), TNFa-eFluor450 (clone MAb11) (eBioscience), IFNg-BV650 (clone 4S.B3) (BioLegend), CD3-BV785 (clone OKT3) (BioLegend) for 30 minutes at room temperature in the dark. All samples were washed thoroughly with PBS and acquired on a CytoFlex flow cytometer (Beckman Coulter).
Thieme C.J., Anft M., Paniskaki K., Blazquez-Navarro A., Doevelaar A., Seibert F.S., Hoelzer B., Konik M.J., Berger M.M., Brenner T., Tempfer C., Watzl C., Meister T.L., Pfaender S., Steinmann E., Dolff S., Dittmer U., Westhoff T.H., Witzke O., Stervbo U., Roch T, & Babel N. (2020). Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients. Cell Reports Medicine, 1(6), 100092.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!