Cxcr4 12g5

Manufactured by Thermo Fisher Scientific

Sourced in United States

CXCR4; 12G5; is a lab equipment product. It functions as a receptor.

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CXCR4 (36 citations)

2 protocols using cxcr4 12g5

Flow Cytometry Characterization of MSCs

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Fluorescence-activated cell sorting flow cytometry analysis was performed using CD29 (TS2/16; eBioscience, San Diego, CA, United States), CD44 (IM7; eBioscience), CD105 (SN6; eBioscience), CD90 (5E10, eBioscience), CD73 (AD2, eBioscience), CD45 (2D1; eBioscience), CD31 (WM-59; eBioscience), CD34 (4H11; eBioscience), HLA-ABC (W6/32; eBioscience), HLA-DR (L243; eBioscience), C-C chemokine receptor type 1 (CCR1; 5F10B29; BioLegend, San Diego, CA, United States), CCR2 (K036C2; BioLegend), CCR7 (3D12; eBioscience), C-X-C chemokine receptor type 4 (CXCR4; 12G5; eBioscience) and CXCR5 (MU5UBEE; eBioscience) antibodies to confirm that the MSC phenotype was maintained after expansion in the culture. The samples were incubated with antibodies against each surface marker for 30 min, and this treatment was followed by fluorescence-activated cell sorting. Flow cytometry analysis was performed on a LSRFortessa (BD Pharmingen, San Diego, CA, United States).

Song Y., Lim J.Y., Lim T., Im K.I., Kim N., Nam Y.S., Jeon Y.W., Shin J.C., Ko H.S., Park I.Y, & Cho S.G. (2020). Human mesenchymal stem cells derived from umbilical cord and bone marrow exert immunomodulatory effects in different mechanisms. World Journal of Stem Cells, 12(9), 1032-1049.

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Detailed Flow Cytometry Antibody Panel

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The following antibodies were used for flow cytometry. For mouse cells, CD4 (RM4-5; eBioscience), CD8a (53-6.7; BD Biosciences), CD90.2 (53-2.1, eBioscience), PD-1 (29F.1A12; BioLegend), CTLA-4 (UC10-4B9, eBioscience), CD25 (PC61; BD Biosciences), CD69 (H1.2F3; BD Biosciences), Foxp3 (FJK-16s; eBioscience), and c-Maf (sym0F1, eBioscience) were used. For human cells, CD4 (OKT4; BioLegend), CXCR4 (12G5; eBioscience), CCR7 (G043H7; BioLegend), CD127 (A019D5; BioLegend), IFN-γ (4S.B3; eBioscience), Foxp3 (236A/E7; eBioscience) CD8 (PRA-T8, BD Biosciences), CTLA4 (BNI3; BioLegend), TIGIT (MBSA43; eBioscience), CD45 (HI30, BD Biosciences), and PD-1 (MIH4; eBioscience) were used.

Seki A, & Rutz S. (2018). Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. The Journal of Experimental Medicine, 215(3), 985-997.

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