Goat anti brn2

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Goat anti-Brn2 is a primary antibody that recognizes the Brn2 (also known as POU3F2) transcription factor. Brn2 is a member of the POU domain family and plays a role in the regulation of gene expression during neural development. The Goat anti-Brn2 antibody can be used to detect and quantify Brn2 in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Rabbit anti tbr1, by Abcam (3 mentions) Rat anti ctip2, by Abcam (2 mentions) Triton x 100, by Merck Group (1 mentions) Mouse anti mbp, by Abcam (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Santa Cruz Biotechnology (1 mentions) Rabbit anti th, by Merck Group (1 mentions) Goat anti chat, by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Rabbit anti mbp, by Abcam (1 mentions) Rabbit anti gaba, by Merck Group (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions) Mouse anti tuj1, by Fortrea (1 mentions) Goat anti sox2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti msi2, by Abcam (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti tuj1, by Merck Group (1 mentions) Lsm710, by Olympus (1 mentions) Rabbit anti dsred, by Thermo Fisher Scientific (1 mentions) Goat anti unc5d, by R&D Systems (1 mentions)

Close spelling variants of this product

Anti-BRN2 (2 citations)

6 protocols using goat anti brn2

Immunofluorescence Staining of Neural Markers

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Cells were fixed with 4% paraformaldehyde (Chemcruz) for 15 min at room temperature, and then washed three times with PBS (Life Genomics). After washing, the fixed cells were permeabilized and blocked with PBS containing 0.03% Triton X-100 (Sigma) and 6% BSA (Sigma) for 1 hr at room temperature. The following primary antibodies were used: goat anti-SOX2 (Santa Cruz, 1：200), goat anti BRN2 (Santa Cruz, 1：200), rabbit anti-BLBP (Santa Cruz, 1：200), rat anti-MSI1 (MBL, 1：200), rabbit anti-MSI2 (Abcam, 1：200), mouse anti-TUJ1 (Covance, 1：500), rabbit anti-GFAP (Dako, 1：500), mouse anti-MBP (Abcam, 1：500), rabbit anti-GABA (Sigma, 1：200), rabbit anti-GLU (Sigma, 1：200), goat-anti ChAT (Merck, 1：200), and rabbit anti-TH (Merck, 1：200)，and rabbit anti-MBP (Abcam, 1：200). Permeabilized cells were incubated with primary antibodies for 16 hrs at 4℃, washed three times with PBS, and incubated with secondary antibodies for 2 hrs at room temperature. Counterstaining was performed with Hoechst 33342 (Sigma).

Kwak T.H., Hali S., Kim S., Kim J., La H., Kim K.P., Hong K.H., Shin C.Y., Kim N.H, & Han D.W. (2020). Robust and Reproducible Generation of Induced Neural Stem Cells from Human Somatic Cells by Defined Factors. International Journal of Stem Cells, 13(1), 80-92.

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Immunohistochemical Analysis of Embryonic and Postnatal Mouse Brain

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Embryos were dissected, and the brains were fixed in 4% paraformaldehyde (PFA) for 1–3.5 hr. For postnatal stage, brains were fixed with 4%PFA overnight. Following 30% sucrose replacement, fixed brains were embedded in optimum cutting temperature (OCT) compound, and 20 micrometer slices were cut on a cryostat. The antibodies used were, rat anti-GFP (1∶500; nakalai tesk), rabbit anti-GFP (1∶200; IBL), rabbit anti-DsRed (1∶500; Invitrogen), goat anti-Unc5D (1∶100; R&D), rabbit anti-Tbr2 (1∶300; abcam), goat anti-NeuroD1 (1∶100; Santa Cruz), mouse anti-PCNA (1∶100; Cell Signaling), mouse anti-Tuj1 (1∶500; SIGMA), rabbit anti-Tbr1 (1∶100; abcam), mouse anti-RORb (1∶100; PERSEUS PROTEOMICS), rat anti-Ctip2 (1∶300; abcam), goat anti-Brn2 (1∶100; Santa Cruz), and mouse anti-Prdm8 [24] (link). Alexa Fluor-conjugated secondary antibodies (Invitrogen) were also used. EdU labeling (intraperitoneal injection of 12.5 mg/kg EdU) and staining were performed according to manufacturer's instructions (Invitrogen). Stainings were examined with Zeiss LSM 710 or Olympus IX81, and the images were finally processed with Adobe Photoshop.

Inoue M., Kuroda T., Honda A., Komabayashi-Suzuki M., Komai T., Shinkai Y, & Mizutani K.I. (2014). Prdm8 Regulates the Morphological Transition at Multipolar Phase during Neocortical Development. PLoS ONE, 9(1), e86356.

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Serotonin Immunostaining in C. elegans and Mouse Embryos

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C. elegans serotonin antibody staining was performed using the tube fixation protocol (McIntire et al., 1992 (link)). Briefly, synchronized young adult hermaphrodites were fixed in 4% paraformaldehyde (PFA) for 18 hr, with β-mercapto-ethanol for another 18 hr, with 1 mg/ml collagenase (Sigma Aldrich, Merk, Darmstadt, Germany) for 90 min and incubated for 24 hr with rabbit anti-5HT antibody (1:5000; Sigma Aldrich). Alexa 555-conjugated donkey anti-rabbit (1:500; Molecular probes) was used as secondary antibody.
For mouse immunohistochemistry, freshly isolated E11.5 embryos from C57Bl/6JRccHsd were fixed by immersion in 4% PFA. Rabbit anti-Sall2 (1:100; Sigma Aldrich), goat anti-Brn2 (1:100; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-5HT (1:5000; Sigma Aldrich) and goat anti-5HT (1:200; Abcam, Cambridge, UK) antibodies were used. As secondary antibodies Alexa 555-conjugated donkey anti-rabbit and anti-goat, and Alexa 488-conjugated donkey anti-rabbit and anti-goat were used (1:600; Molecular probes, Invitrogen, Eugene, OR). Immunofluorescence samples were analyzed and photographed using a confocal TCS-SP8 Leica microscope.

Lloret-Fernández C., Maicas M., Mora-Martínez C., Artacho A., Jimeno-Martín Á., Chirivella L., Weinberg P, & Flames N. (2018). A transcription factor collective defines the HSN serotonergic neuron regulatory landscape. eLife, 7, e32785.

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Protein Expression Analysis in Forebrain Tissue

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Protein was extracted from forebrain tissue (P7 and P10) using RIPA buffer for 30 min on ice (for TBR1 and CTIP2), or using the NE-PER nuclear protein extraction kit (for BRN2) (Thermo scientific). 10–30 µg of protein was resolved on 8% SDS-PAGE and transferred onto nitrocellulose membranes (Bio-Rad Laboratories). Antibodies used were goat anti-BRN2 (1:1000, Santa Cruz Biotechnology, Inc.), rabbit anti-TBR1 (1:1000, Abcam), and rabbit anti-CTIP2 (1:1000, Abcam) followed by the appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:5,000, GE Healthcare). The membranes were incubated in enhanced chemiluminescence reagent (ECL) before exposure to x-ray film (Amersham). The membranes were reprobed with mouse anti-α-tubulin (1:4,000, Sigma–Aldrich) or anti-INCENP as a loading control.

Ritchie K., Watson L.A., Davidson B., Jiang Y, & Bérubé N.G. (2014). ATRX is required for maintenance of the neuroprogenitor cell pool in the embryonic mouse brain. Biology Open, 3(12), 1158-1163.

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Immunohistochemical Labeling of Cortical Neurons

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At postnatal day 1 (P1) or embryo day 18.5 (E18.5), the brains were fixed with 4% paraformaldehyde (PFA) for 48 h at 4℃. Brains were cut in 50 μm sections with a Leica VT 1000S vibratome (Leica Microsystems). The tissue sections were incubated with blocking solution (containing 0.1 M PBS, 5% serum, 1% BSA and 0.2% Triton X-100) at RT for 2 h. The sections were incubated with the rabbit-anti-GFP (1：1,000, Millipore) and goat-anti-Brn2 (1：1,000, Santa Cruz Biotechnology Inc.) overnight at 4℃. After rinsing in 0.1 M PBS, the sections were incubated with donkey-anti-rabbit 488 (1：300, Millipore) and donkey-anti-goat 555 (1：300, Millipore) for 3 h at RT. Then, the sections were counterstained with DAPI for 20 min and rinsed in 0.1 M PBS for 1h. The sections were mounted in Dako Fluorescent Mounting Medium on glass slides and placed overnight at 4℃.

Lu X., Hu X., Song L., An L., Duan M., Chen S, & Zhao S. (2015). The SH2 domain is crucial for function of Fyn in neuronal migration and cortical lamination. BMB Reports, 48(2), 97-102.

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Immunostaining of Neuronal Cell Cultures

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Immunostaining of cultures and brain sections were performed as previously described.^{102 (link)} In brief, cultures were fixed in 4% (w/v) paraformaldehyde for 10 min, washed in PBS and incubated overnight in PBS-Azide (0.02% w/v) solution containing 10% (w/v) normal donkey serum (NDS), 0.3% Triton-X and primary antibodies rabbit anti-TBR1 (1:1000; Abcam), rat anti-CTIP2 (1:500; Abcam), and goat anti-BRN2 (1:200, Santa Cruz). The following day, cells were washed, blocked with 10% NDS and incubated in secondary antibodies (AlexaFluor-488, −555 or −647; Jackson ImmunoResearch) for 1.5 hrs. Nuclei were counterstained with DAPI. Images were captured on a Zeiss Axio ObserverZ.1 inverted epifluorescence microscope at 20x and the total number of DAPI, TBR1, CTIP2, and BRN2 immunolabeled cells quantified (Figure 6F).

Pantazis C.B., Yang A., Lara E., McDonough J.A., Blauwendraat C., Peng L., Oguro H., Kanaujiya J., Zou J., Sebesta D., Pratt G., Cross E., Blockwick J., Buxton P., Kinner-Bibeau L., Medura C., Tompkins C., Hughes S., Santiana M., Faghri F., Nalls M.A., Vitale D., Ballard S., Qi Y.A., Ramos D.M., Anderson K.M., Stadler J., Narayan P., Papademetriou J., Reilly L., Nelson M.P., Aggarwal S., Rosen L.U., Kirwan P., Pisupati V., Coon S.L., Scholz S.W., Priebe T., Öttl M., Dong J., Meijer M., Janssen L.J., Lourenco V.S., van der Kant R., Crusius D., Paquet D., Raulin A.C., Bu G., Held A., Wainger B.J., Gabriele R.M., Casey J.M., Wray S., Abu-Bonsrah D., Parish C.L., Beccari M.S., Cleveland D.W., Li E., Rose I.V., Kampmann M., Aristoy C.C., Verstreken P., Heinrich L., Chen M.Y., Schüle B., Dou D., Holzbaur E.L., Zanellati M.C., Basundra R., Deshmukh M., Cohen S., Khanna R., Raman M., Nevin Z.S., Matia M., Van Lent J., Timmerman V., Conklin B.R., Chase K.J., Zhang K., Funes S., Bosco D.A., Erlebach L., Welzer M., Kronenberg-Versteeg D., Lyu G., Arenas E., Coccia E., Sarrafha L., Ahfeldt T., Marioni J.C., Skarnes W.C., Cookson M.R., Ward M.E, & Merkle F.T. (2022). A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell stem cell, 29(12), 1685-1702.e22.

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