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Tramp c2

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TRAMP-C2 is a cell line derived from a human prostate tumor. It is used in research related to prostate cancer.

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Lab products found in correlation

Du145, by American Type Culture Collection (12 mentions) Lncap, by American Type Culture Collection (6 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Myc cap, by American Type Culture Collection (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Renca, by American Type Culture Collection (3 mentions) Tramp c1, by American Type Culture Collection (3 mentions) Hek293, by American Type Culture Collection (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) Murine b16 melanoma cell line, by American Type Culture Collection (2 mentions) Tramp c3, by American Type Culture Collection (2 mentions) Hek293 cell, by American Type Culture Collection (2 mentions) Pan02, by Merck Group (2 mentions) Dihydrotestosterone, by Merck Group (2 mentions) Insulin, by Merck Group (2 mentions) Bovine insulin, by Merck Group (2 mentions) Nu serum 4, by Corning (2 mentions) Rwpe 1, by American Type Culture Collection (2 mentions) Bph 1, by American Type Culture Collection (2 mentions) Primary human prostate epithelial cells pcs 440 010, by American Type Culture Collection (2 mentions)

28 protocols using tramp c2

1

AAV-Mediated Cell Transduction Assay

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For AAV particle transduction experiments, cells were plated in ViewPlate-96 Black (PerkinElmer®, Waltham, MA, USA) at cell type specific densities to ensure no overgrowth. The cells were infected in 1/3 dilutions of 150,000 VG/cell to 5000 VG/cell. Successful transduction was analyzed via IncuCyte® S3 Live-Cell Analysis System (Sartorius, Göttingen, Germany) and flow cytometry after three days. Cell lines 4T1, B16-F10, bEND.3, CT26-CL25, NIH3T3, Renca, Tramp C2, and FL8-3B were purchased from ATCC (Manassas, VA, USA). MC-38 cells were purchased from NIH/NCI (Bethesda, MD, USA).
Kuklik J., Michelfelder S., Schiele F., Kreuz S., Lamla T., Müller P, & Park J.E. (2021). Development of a Bispecific Antibody-Based Platform for Retargeting of Capsid Modified AAV Vectors. International Journal of Molecular Sciences, 22(15), 8355.
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2

Characterization of Prostate Cancer Cell Lines

Cited 2 times
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PC3 cells were a generous gift from Dr. Carrie Rinker-Schaeffer. TRAMPC2, DU145, and LNCaP cells were purchased from ATCC, and ARCaPM obtained from Novicure Biotechnology. PC3 and ARCaPM cell line variants were generated as previously described [9 (link)]. Antibodies used for Western blot analysis or multiplexed quantum dot labeling (mQDL) were: anti-FYN antibody (Cell Signaling #4023 or Santa Cruz #sc16), phospho-MET (#3126) and total MET (#4560) obtained from Cell Signaling. Anti-CD44 (sc-7297); anti-CD56 (sc-7326); anti-CHGA (sc-13090), anti-SYP (sc-17750), all obtained from Santa Cruz. Other common reagents for mQDL were used as described previously [39 , 40 (link)]. Recombinant human HGF was purchased from Calbiochem (Millipore).
Gururajan M., Cavassani K.A., Sievert M., Duan P., Lichterman J., Huang J.M., Smith B., You S., Nandana S., Chu G.C., Mink S., Josson S., Liu C., Morello M., Jones L.W., Kim J., Freeman M.R., Bhowmick N., Zhau H.E., Chung L.W, & Posadas E.M. (2015). SRC family kinase FYN promotes the neuroendocrine phenotype and visceral metastasis in advanced prostate cancer. Oncotarget, 6(42), 44072-44083.
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3

Murine Models for Lysosomal Acid Lipase

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lal+/+ and lal−/− mice of the FVBN background were bred in house. c-fms-rtTA/(TetO)7-CMV-hLAL; lal−/−(Tg/KO) triple mice of the FVBN background is a previously generated triple transgenic mouse model with myeloid-specific doxycycline-inducible expression of wild-type human LAL (hLAL) in lal−/− mice under the control of the c-fms promoter 3 (link), 7 (link). All scientific protocols involving the use of animals have been approved by the Institutional Animal Care and Use Committee of Indiana University School of Medicine and followed guidelines established by the Panel on Euthanasia of the American Veterinary Medical Association. Animals were housed under Institutional Animal Care and Use Committee-approved conditions in a secured animal facility at Indiana University School of Medicine.
The murine B16 melanoma cell line, Lewis lung carcinoma (LLC) cell line, and transgenic mouse prostate cancer (TRAMP-C2) cell line (purchased from ATCC) were cultured in DMEM supplemented with 10% FBS (Gibco).
Zhao T., Du H., Ding X., Walls K, & Yan C. (2014). Activation of mTOR Pathway in Myeloid-derived Suppressor Cells Stimulates Cancer Cell Proliferation and Metastasis in lal−/− Mice. Oncogene, 34(15), 1938-1948.
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4

Murine Models for Lysosomal Acid Lipase

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lal+/+ and lal−/− mice of the FVBN background were bred in house. c-fms-rtTA/(TetO)7-CMV-hLAL; lal−/−(Tg/KO) triple mice of the FVBN background is a previously generated triple transgenic mouse model with myeloid-specific doxycycline-inducible expression of wild-type human LAL (hLAL) in lal−/− mice under the control of the c-fms promoter 3 (link), 7 (link). All scientific protocols involving the use of animals have been approved by the Institutional Animal Care and Use Committee of Indiana University School of Medicine and followed guidelines established by the Panel on Euthanasia of the American Veterinary Medical Association. Animals were housed under Institutional Animal Care and Use Committee-approved conditions in a secured animal facility at Indiana University School of Medicine.
The murine B16 melanoma cell line, Lewis lung carcinoma (LLC) cell line, and transgenic mouse prostate cancer (TRAMP-C2) cell line (purchased from ATCC) were cultured in DMEM supplemented with 10% FBS (Gibco).
Zhao T., Du H., Ding X., Walls K, & Yan C. (2014). Activation of mTOR Pathway in Myeloid-derived Suppressor Cells Stimulates Cancer Cell Proliferation and Metastasis in lal−/− Mice. Oncogene, 34(15), 1938-1948.
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5

Prostate Cancer Cell Lines Study

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The following cell lines were used in these studies: CV1 Monkey kidney fibroblasts (ATCC, Manassas, VA, USA); Human prostate cancer cell lines PC3, DU145, LNCaP and WPMY-1 (Obtained from the laboratory of Professor Pandha, University of Surrey, UK). Murine prostate cancer cell lines TRAMP-C1 (ATCC), TRAMP-C2 (Obtained from the laboratory of Professor Vile, Mayo Clinic) and TRAMP-C3 (ATCC).
Mansfield D.C., Kyula J.N., Rosenfelder N., Chao-Chu J., Kramer-Marek G., Khan A.A., Roulstone V., McLaughlin M., Melcher A.A., Vile R.G., Pandha H.S., Khoo V, & Harrington K.J. (2016). Oncolytic vaccinia virus as a vector for therapeutic sodium iodide symporter gene therapy in prostate cancer. Gene Therapy, 23(4), 357-368.
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6

TRAMP-C2 Prostate Tumor Implantation

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Specific pathogen free C57BL/6 mice and C3H mice, 6 to 8 weeks of age, were purchased from Taconic Farms (Germantown, NY). TRAMP-C2 (ATCC CRL-2731; originally derived from the prostate tumor of a TRAMP mouse on the C57BL/6 background) cells were used for tumor challenge studies. TRAMP-C2 cells were grown and expanded in vitro with IMDM medium supplemented with 5% Fetal bovine serum (FBS; Gemini, Sacramento, CA), 5% Nu Serum IV (BD Biosciences, San Jose, CA), 0.01 nM dihydrotestosterone (Sigma Chemical Co.), and 5 μg/ml insulin (Sigma Chemical Co.). All in vivo studies were in compliance and approved by University of Southern California Institutional Animal Care and Use Committee (USC IACUC).
Yan L., Da Silva D.M., Verma B., Gray A., Brand H.E., Skeate J.G., Porras T.B., Kanodia S, & Kast W.M. (2014). Forced LIGHT expression in prostate tumors overcomes Treg mediated immunosuppression and synergizes with a prostate tumor therapeutic vaccine by recruiting effector T lymphocytes. The Prostate, 75(3), 280-291.
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7

Prostate Cancer Cell Lines and Adenoviral Vectors

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Human prostate tumor cell lines PC-3 and DU-145, and a mouse prostate tumor cell line TRAMP-C2 were obtained from ATCC (Manassas, VA). PC-3-luc cell line was kindly provided by Kenneth Pienta (University of Michigan, Ann Arbor, MI). All prostate tumor cell lines were maintained in RPMI-1640 media containing 10% fetal calf serum. To create Ad.dcn, the decorin gene was cloned in a shuttle vector and subjected to homologous recombination with adenoviral genomic DNA derived from adenoviral mutant dl01/07, using published methods.41 (link) To create Ad(E1-).dcn, an AdEasy system was used for homologous recombination.42 (link) Ad(E1-).null (the non-replicating adenovirus without any foreign trangene), Ad(E1-).luc (the non-replicating adenovirus containing firefly luciferase2 gene), and Ad.luc (the conditionally replicating adenovirus containing firefly luciferase2 gene) have been previously described.33 (link), 43 (link), 44 (link) All adenoviral vectors were amplified in HEK293 cells (ATCC), and purified as described earlier.43 (link)
Xu W., Neill T., Yang Y., Hu Z., Cleveland E., Wu Y., Hutten R., Xiao X., Stock S.R., Shevrin D., Kaul K., Brendler C., Iozzo R.V, & Seth P. (2014). The systemic delivery of an oncolytic adenovirus expressing decorin inhibits bone metastasis in a mouse model of human prostate cancer. Gene therapy, 22(3), 247-256.
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8

Prostate Cancer Cell Culture Protocol

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Male-derived murine TRAMP-C2 and TRAMP-C3 prostate cancer cell lines were purchased from ATCC, maintained and cultured according to their suggested protocols.
El-Kenawi A., Gatenbee C., Robertson-Tessi M., Bravo R., Dhillon J., Balagurunathan Y., Berglund A., Vishvakarma N., Ibrahim-Hashim A., Choi J., Luddy K., Gatenby R., Pilon-Thomas S., Anderson A., Ruffell B, & Gillies R. (2019). Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer. British Journal of Cancer, 121(7), 556-566.
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9

Comparative CaP Cell Line Characterization

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Human and murine cell models representing either primary or metastatic CaP disease were selected for the study. TRAMP-C2 (mouse primary CaP; RRID:CVCL_3615), Myc-CaP (mouse primary CaP; RRID:CVCL_J703), PC-3 (human bone-derived metastatic CaP; ATCC#CRL-7934, RRID:CVCL_0035), DU145 (human brain-derived metastatic CaP; (ATCC#HTB-81, RRID:CVCL_0105),), 22Rν1 (CRPC cell model) and mesenchymal stem cells (MSCs) were used in this study. The PC-3, DU145 TRAMP-C2 and mouse MSC cell models were procured from ATCC (Manassas, VA) whereas hMSC was obtained from PromoCell (USA). Myc-CaP was procured from Dr. Charles Sawyer`s laboratory (Memorial Sloan Kettering Cancer center, New York, NY). DU145, TRAMP-C2 and Myc-CaP were grown in DMEM whereas PC-3 cells were grown in RPMI +10% FBS. MSCs were grown in MesenCulT™ MSC Basal Media and Mesencult™ Osteogenic Stimulatory Supplement. All the human cells were tested for contamination and genetic accuracy using STR analysis (Genetica Cell Line Testing, Burlington, USA).
Ganaie A.A., Mansini A.P., Hussain T., Rao A., Siddique H.R., Shabaneh A., Ferrari M.G., Murugan P., Klingelhöfer J., Wang J., Ambartsumian N., Warlick C.A., Konety B.R, & Saleem M. (2020). Anti-S100A4 antibody therapy is efficient in treating aggressive prostate cancer and reversing immunosuppression: Serum and biopsy S100A4 as clinical predictor. Molecular cancer therapeutics, 19(12), 2598-2611.
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10

Prostate Cancer Cell Lines and Adenoviral Vectors

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Human prostate tumor cell lines PC-3 and DU-145, and a mouse prostate tumor cell line TRAMP-C2 were obtained from ATCC (Manassas, VA). PC-3-luc cell line was kindly provided by Kenneth Pienta (University of Michigan, Ann Arbor, MI). All prostate tumor cell lines were maintained in RPMI-1640 media containing 10% fetal calf serum. To create Ad.dcn, the decorin gene was cloned in a shuttle vector and subjected to homologous recombination with adenoviral genomic DNA derived from adenoviral mutant dl01/07, using published methods.41 (link) To create Ad(E1-).dcn, an AdEasy system was used for homologous recombination.42 (link) Ad(E1-).null (the non-replicating adenovirus without any foreign trangene), Ad(E1-).luc (the non-replicating adenovirus containing firefly luciferase2 gene), and Ad.luc (the conditionally replicating adenovirus containing firefly luciferase2 gene) have been previously described.33 (link), 43 (link), 44 (link) All adenoviral vectors were amplified in HEK293 cells (ATCC), and purified as described earlier.43 (link)
Xu W., Neill T., Yang Y., Hu Z., Cleveland E., Wu Y., Hutten R., Xiao X., Stock S.R., Shevrin D., Kaul K., Brendler C., Iozzo R.V, & Seth P. (2014). The systemic delivery of an oncolytic adenovirus expressing decorin inhibits bone metastasis in a mouse model of human prostate cancer. Gene therapy, 22(3), 247-256.
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