Wild type C57BL/6 (Jackson # 000664), Casp1−/− (Jackson # 032662), Casp1−/− (Kayagaki et al., 2011 (link)), Casp1-Casp11DKO (Kuida et al., 1995 (link)), Nlrc4-AscDKO (Aachoui et al., 2015 (link)), Gsdmd−/− (Jackson # 032663), Gsdme−/− (Jackson # 032411), Mefv−/− (Chae et al., 2011 (link)), and Padi4−/− (Jackson # 030315) mice were used in this study. We crossed Gsdmd−/− with Gsdme−/− to generate Gsdmd-GsdmeDKO. All mice used in this study were in C57BL/6 genetic background and were bred and housed up to five mice per ventilated cage in a specific pathogen-free facility at the University of Arkansas for Medical Sciences. Both female and male mice aged between 6 and 10 weeks were used for all animal experiments. All protocols met the guidelines of the US National Institutes of Health for the humane care of animals and were approved by the Institutional Animal Care and Use Committee at the University of Arkansas for Medical Sciences.
Gsdme
Manufactured by Jackson ImmunoResearch
Gsdme−/− is a laboratory mouse strain with a targeted deletion of the Gsdme gene. The Gsdme gene encodes for the Gasdermin E protein, which is involved in cell death pathways. This mouse strain can be used by researchers to study the role of Gasdermin E in various biological processes.
Automatically generated - may contain errors
3 protocols using gsdme
1
Genetic Mouse Models for Inflammasome Research
2
Genetic Mouse Models for Inflammasome Research
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Wild type C57BL/6 (Jackson # 000664), Casp1−/− (Jackson # 032662), Casp1−/− (Kayagaki et al., 2011 (link)), Casp1-Casp11DKO (Kuida et al., 1995 (link)), Nlrc4-AscDKO (Aachoui et al., 2015 (link)), Gsdmd−/− (Jackson # 032663), Gsdme−/− (Jackson # 032411), Mefv−/− (Chae et al., 2011 (link)), and Padi4−/− (Jackson # 030315) mice were used in this study. We crossed Gsdmd−/− with Gsdme−/− to generate Gsdmd-GsdmeDKO. All mice used in this study were in C57BL/6 genetic background and were bred and housed up to five mice per ventilated cage in a specific pathogen-free facility at the University of Arkansas for Medical Sciences. Both female and male mice aged between 6 and 10 weeks were used for all animal experiments. All protocols met the guidelines of the US National Institutes of Health for the humane care of animals and were approved by the Institutional Animal Care and Use Committee at the University of Arkansas for Medical Sciences.
3
Genetically Engineered Mice for Inflammasome Studies
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C57BL/6, Gsdme-/- and Nlrp3-/- mice were purchased from the Jackson Laboratories. Gsdmd-/- and Nlrc4-/- mice were a kind gift from Dr. Russell Vance (University of California, Berkeley). Gsdmd-/-/Gsdme-/- mice were generated in-house. All transgenic mice were on a C57BL/6 background. As mice were bred in-house, we used approximately equal numbers of males and females, and ordered male and female mice from Jackson Laboratories. Animals were housed in pathogen free conditions in microisolator cages and were treated according to institutional guidelines following approval by the University of California, Irvine IACUC. We routinely use male and female mice aged 6-10 weeks. Although we noted the sex of each mice, we did not note any gender differences using mice as a source of cells, or in infection studies.
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